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A metal-organic framework polycation derivative and its preparation method and application

A metal-organic framework and polycation technology, applied in the field of metal-organic framework polycationic derivatives and their preparation, can solve the problems of cationic polymer nucleic acid introduction efficiency, insufficient gene expression efficiency, etc., and achieve good application prospects and low cytotoxicity. , the effect of low cost

Active Publication Date: 2020-08-11
深圳近邻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nucleic acid introduction efficiency and gene expression efficiency of these cationic polymers are not sufficient.

Method used

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  • A metal-organic framework polycation derivative and its preparation method and application
  • A metal-organic framework polycation derivative and its preparation method and application
  • A metal-organic framework polycation derivative and its preparation method and application

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Experimental program
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Embodiment 1

[0037] by figure 1 As shown, this embodiment provides a preparation method of UiO-PGMA-EA, and the specific steps are as follows:

[0038] NH 2 -UiO-66→UiO-BiBB→UiO-PGMA→UiO-PGMA-EA

[0039] (1) Preparation of metal atom nanocrystal core NH 2 -UiO-66;

[0040] 2-Aminoterephthalic acid (0.248 g, 1.372 mmol) and zirconium tetrachloride (0.320 g, 1.372 mmol) were dissolved in 80 mL of dimethylformamide. Mix the above mixed solution with 1.236 mL of pure water and seal. The reaction was carried out under nitrogen as the protective gas at a constant temperature of 120°C for 24 hours. The reaction solution was cooled to 4°C, the precipitate was collected, and washed with low-temperature dimethylformamide and methanol solution to obtain the purified product NH 2 -UiO-66. The precipitated product is finally vacuum dried to remove solvent molecules.

[0041] (2) UiO-BiBB, the initiator of chemical bond introduction into polymer reaction:

[0042] Will get the NH 2 -UiO-66 (0.30 g) was disso...

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Abstract

The invention relates to a metallic organic framework polycation derivative, a preparation method and application of the derivative. The derivative takes a metallic atom as a nanometer crystal core and a cationic polymer chain segment as a nanometer crystal shell. The derivative is used for delivering nucleic acids to target cells or tissues. As a nucleic acid delivery material, the metallic organic framework polycation derivative is capable of supplying high-strength nucleic acid binding force, forming a nucleic acid compound with high colloidal stability, supplying efficient nucleic acid guiding efficiency and efficient gene expression efficiency and guaranteeing lower cytotoxicity. The preparation method is simple, the cost is low and the application prospect is good.

Description

Technical field [0001] The invention belongs to the field of nucleic acid delivery materials, and particularly relates to a metal organic framework polycation derivative, and a preparation method and application thereof. Background technique [0002] Currently, in nucleic acid therapy, viral vectors and synthetic vectors (non-viral vectors) have been studied as vectors for delivering nucleic acids to target cells or tissues. [0003] It is generally believed that synthetic vectors are the same as the existing drug delivery systems (DDS) studied in medical treatment. Although there are risks involved in toxicity, if compared with viral vectors, they are less toxic and have no limitation on the size of the nucleic acid carried , And can carry out precise molecular design of the carrier, therefore, continue to carry out vigorous development and research. As a representative synthetic carrier, cationic liposomes or cationic polymers that form an ionic complex with negatively charged D...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00C12N15/87
CPCC08G83/008C12N15/87
Inventor 陈麒先
Owner 深圳近邻生物科技有限公司