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Biological chip

A biochip and chip technology, which is applied in the direction of biomass post-treatment, biomass pretreatment, biochemical cleaning device, etc., can solve the problems of long detection time and many detection steps, and achieve low manufacturing cost, high detection efficiency, and structure simple effect

Active Publication Date: 2017-12-01
杨华卫 +2
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, in the whole detection process of traditional chips, there are many detection steps and long detection time.

Method used

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  • Biological chip
  • Biological chip
  • Biological chip

Examples

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Effect test

example 1

[0041] In this example, using the figure 1 The biochip shown was used for mycobacterial identification, detecting 4 kinds of mycobacterial nucleic acids. There are many kinds of mycobacteria that can cause tuberculosis clinically, among which the common ones are Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium kansasii.

[0042] In this example, the chip reaction interface is a glass chip, and the detection signal adopts a fluorescent labeling method. In this example, the slide on which the mycobacteria probes are immobilized is embedded on one side of the chip handle, in a mounting hole provided in the region close to its free end. During the detection, slides are soaked in the solution for chip hybridization, washing and fluorescent signal scanning and other operations.

[0043] The following describes how to use figure 1 Each step of the method for the identification and identification of mycobacteria is carried out with the...

example 2

[0079] In this example, using the image 3 The chip stalk is shown to construct the biochip for M. tuberculosis identification. A nitrocellulose membrane immobilized with mycobacterial probes is attached to the end of a plastic rod that can be folded over to clamp the membrane chip. During detection, the end is soaked in the solution to complete the processes of chip hybridization, washing and color development. The relevant parameters are as follows: nucleic acid membrane array, using 2 detection points, including 2 detection nucleic acid probes (Mycobacterium probe, Mycobacterium tuberculosis probe); colored spots, the results are interpreted by naked eyes.

[0080] The following describes how to use image 3 The steps of the method for the identification of Mycobacterium tuberculosis are performed on the chip-stalk-structured biocore.

[0081] Step 1: Fabrication of Membrane Chip

[0082] Design and synthesis of mycobacterial probes:

[0083] Mycobacterium probe sequen...

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Abstract

The invention relates to a biological chip, and belongs to the technical field of biological detection. The biological chip provided by the invention comprises a base plate, a plurality of chip stems and chip reaction interfaces, wherein the plurality of chip stems are arranged on the base plate in the form of an array; the chip stems, in a direction perpendicular to the base plate, extend from the base plate in a mode of being mutually parallel; the chip reaction interfaces are arranged at the free ends of the various chip stems and are used for marking detection probes; and the chip reaction interfaces are longitudinally distributed to be longitudinally overlapped with the chip stems. The biological chip, when used for conducting detection, can save a great amount of detection steps and operation time. By vertically the chip reaction interfaces at the ends of the chip stems, more reaction tanks and chips can be distributed on unit area, so that the quantity of detected samples is increased, and subsequently, a high detection efficiency is guaranteed. When the detected clinical samples are at a relatively low quantity, detection can be completed by virtue of detection equipment which is smaller in volume, so that the detection is facilitated, and cost is also reduced.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a biological chip. Background technique [0002] In the detection of traditional membrane chips, it is necessary to add each detection sample one by one into the wells or grooves of the chip for incubation. Then, the incubated samples are removed from the wells or grooves of the chip one by one, and the washing solution is added to the wells or grooves used for detection several times in sequence for washing. After washing, the reaction liquid is sequentially added to the wells or grooves for reaction, and the reacted liquid needs to be removed one by one. After removing the reacted liquid, it is necessary to wash the wells or grooves used for detection several times, remove the liquid in the wells or grooves, and keep them dry. After that, it is necessary to sequentially add the liquid as the detection signal into the wells or grooves one by one to car...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCB01L3/5085
Inventor 杨华卫曾冀杨化莉
Owner 杨华卫
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