Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria

A high-efficiency technology for degrading bacteria and polycyclic aromatic hydrocarbons. It is applied in the direction of using microorganisms, methods based on microorganisms, and the restoration of contaminated soil. It can solve the problems of environmental secondary pollution, incomplete restoration, and high cost. The method is simple , good degradation ability, high application value effect

Active Publication Date: 2017-12-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both physical and chemical repair technologies have the problems of high cost, incomplete repair, and easy to caus

Method used

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  • Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria
  • Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria
  • Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria

Examples

Experimental program
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Example Embodiment

[0073] Example 1. A method for screening high-efficiency phenanthrene-degrading bacteria from soil, followed by the following steps:

[0074] 1) Weigh 5 g of a PAHs-contaminated soil in Sanming, Fujian, resuspend it in 45 mL of sterilized water, and place it in a 250 mL sterilized Erlenmeyer flask with shaking (28° C., 130 rpm) for 2 hours to obtain a soil suspension.

[0075] 2) Pipette 1mL of the soil suspension obtained in step 1) into 9mL phenanthrene-degrading bacteria acclimation medium (the concentration of phenanthrene is 100mg -1 ), shaking culture in a constant temperature shaker at 28°C and 130 rpm for 14 days; the soil suspension after acclimatization was obtained.

[0076] 3) Dilute the domesticated soil suspension obtained in step 2) by 10 3 , 10 4 , 10 5 Double (volume times), each draw 200μL of the dilution and spread it on the phenanthrene-degrading bacteria screening plate, and place it in a 28°C incubator, and observe whether there are colonies on the plate every da...

Example Embodiment

[0090] Example 2. A method for screening high-efficiency pyrene-degrading bacteria from soil, followed by the following steps:

[0091] 1). Weigh 5g of PAHs-contaminated soil in Sanming City, Fujian, resuspend it in 45mL sterilized water, and place it in a 250mL sterilized Erlenmeyer flask with shaking (28°C, 130rpm) for 2 hours to obtain a soil suspension.

[0092] 2) Draw 1mL 1) Add the soil suspension obtained in step 1) to 9mL pyrene degrading bacteria acclimation medium (the pyrene concentration is 100mg L -1 ), shaking culture in a constant temperature shaker at 28°C and 130 rpm for 14 days; the soil suspension after acclimatization was obtained.

[0093] 3). Dilute the soil suspension obtained in step 2) by 10 3 , 10 4 , 10 5 Take 200μL of each dilution and spread it on the pyrene-degrading bacteria screening plate, place it in a 28°C incubator, and observe whether there is colony formation on the plate every day.

[0094] 4). Select a single colony with a transparent degradatio...

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Abstract

The invention discloses an efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria WY6 (Mycobacterium sp.WY6) with the preservation number being CCTCC (China Center for Type Culture Collection) NO:M 2017373. The invention further provides an application of the efficient PAH degrading bacteria WY6. The efficient PAH degrading bacteria WY6 is used for controlling high-concentration PAH pollution of soil; the rate of degradation of phenanthrene with concentration being 100 mg L<-1> reaches 99.75%, and the rate of degradation of pyrene with concentration being 50 mg L<-1> reaches 98.73%. The invention further provides a method for screening the efficient PAH degrading bacteria.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of environmental organic pollutants, and relates to a method for efficiently screening polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) degrading bacteria and an obtained high-efficiency polycyclic aromatic hydrocarbons degrading bacteria Mycobacterium sp.WY6. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a typical class of persistent organic pollutants, which are formed by the fusion of two or more benzene rings. It mainly comes from coal and petroleum combustion, smelting and transportation industries, etc. It can exist stably in the environment for a long time, and is widely distributed in the atmosphere, water and soil. Because of its "carcinogenic, teratogenic and mutagenic" characteristics, it has recently It has attracted widespread public attention, and it is also one of the problems that r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C12N1/02B09C1/10C12R1/32
CPCB09C1/10C12N1/02C12N1/20C12N1/36C12N1/205C12R2001/32
Inventor 汪海珍杨黎陈远志楼骏徐建明
Owner ZHEJIANG UNIV
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