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Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria

A high-efficiency technology for degrading bacteria and polycyclic aromatic hydrocarbons. It is applied in the direction of using microorganisms, methods based on microorganisms, and the restoration of contaminated soil. It can solve the problems of environmental secondary pollution, incomplete restoration, and high cost. The method is simple , good degradation ability, high application value effect

Active Publication Date: 2017-12-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both physical and chemical repair technologies have the problems of high cost, incomplete repair, and easy to cause secondary pollution to the environment. It is difficult to promote on a large scale and has certain restrictions.

Method used

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  • Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria
  • Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria
  • Method for screening efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria and obtained efficient degrading bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, a kind of method for screening phenanthrene highly efficient degrading bacteria from soil, carries out following steps successively:

[0074] 1) Weigh 5 g of PAHs-contaminated soil collected from a certain PAHs-contaminated soil in Sanming City, Fujian, resuspend in 45 mL of sterilized water, place in a 250 mL sterilized Erlenmeyer flask for shaking (28°C, 130 rpm) and incubate for 2 hours to obtain a soil suspension.

[0075] 2) Draw 1 mL of the soil suspension obtained in step 1) and add it to 9 mL of the phenanthrene-degrading bacteria acclimation medium (the concentration of phenanthrene is 100 mg L -1 ) in a constant temperature shaker at 28°C and 130rpm for 14 days; the soil suspension after acclimatization culture was obtained.

[0076] 3) Dilute the domesticated cultured soil suspension obtained in step 2) by 10 respectively. 3 、10 4 、10 5 times (volume times), each pipette 200 μL of the dilution and spread it on a phenanthrene-degrading bacteri...

Embodiment 2

[0090] Embodiment 2, the method for screening pyrene efficient degrading bacteria from soil, carries out following steps successively:

[0091] 1) Weigh 5g of PAHs-contaminated soil collected in Sanming City, Fujian Province, resuspend in 45mL sterilized water, place in a 250mL sterilized Erlenmeyer flask for shaking (28°C, 130rpm) and incubate for 2 hours to obtain a soil suspension.

[0092] 2), draw 1mL of the soil suspension obtained in step 1) and add it to 9mL of pyrene-degrading bacteria acclimation medium (pyrene concentration is 100mg L -1 ) in a constant temperature shaker at 28°C and 130rpm for 14 days; the soil suspension after acclimatization culture was obtained.

[0093] 3), the soil suspension after step 2) gained acclimation is diluted 10 respectively 3 、10 4 、10 5 Each time, 200 μL of the diluted solution was drawn and spread on a screening plate for pyrene-degrading bacteria, placed in a 28°C incubator for cultivation, and observed daily whether there wer...

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Abstract

The invention discloses an efficient PAH (polycyclic aromatic hydrocarbon) degrading bacteria WY6 (Mycobacterium sp.WY6) with the preservation number being CCTCC (China Center for Type Culture Collection) NO:M 2017373. The invention further provides an application of the efficient PAH degrading bacteria WY6. The efficient PAH degrading bacteria WY6 is used for controlling high-concentration PAH pollution of soil; the rate of degradation of phenanthrene with concentration being 100 mg L<-1> reaches 99.75%, and the rate of degradation of pyrene with concentration being 50 mg L<-1> reaches 98.73%. The invention further provides a method for screening the efficient PAH degrading bacteria.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of environmental organic pollutants, and relates to a method for efficiently screening polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) degrading bacteria and an obtained high-efficiency polycyclic aromatic hydrocarbons degrading bacteria Mycobacterium sp.WY6. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a typical class of persistent organic pollutants, which are formed by the fusion of two or more benzene rings. It mainly comes from coal and petroleum combustion, smelting and transportation industries, etc. It can exist stably in the environment for a long time, and is widely distributed in the atmosphere, water and soil. Because of its "carcinogenic, teratogenic and mutagenic" characteristics, it has recently It has attracted widespread public attention, and it is also one of the problems that r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C12N1/02B09C1/10C12R1/32
CPCB09C1/10C12N1/02C12N1/20C12N1/36C12N1/205C12R2001/32
Inventor 汪海珍杨黎陈远志楼骏徐建明
Owner ZHEJIANG UNIV
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