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Application of protein marker of multiple sclerosis

A multiple sclerosis and protein technology, applied in the field of multiple sclerosis protein markers, can solve problems such as complex clinical manifestations

Inactive Publication Date: 2017-12-05
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex clinical manifestations of the disease, there is still a lack of methods for the specific diagnosis of multiple sclerosis in the early stages of the disease

Method used

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  • Application of protein marker of multiple sclerosis
  • Application of protein marker of multiple sclerosis
  • Application of protein marker of multiple sclerosis

Examples

Experimental program
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Effect test

Embodiment

[0031] Materials and Reagents

[0032] 1) Instrument: rat metabolic cage: purchased from Beijing Jiayuan Xingye Technology Co., Ltd. TripleTOF 5600 mass spectrometer: purchased from ABI Company; Waters UPLC high-performance liquid chromatograph: purchased from Waters Company; MillliQ RG ultrapure water system: purchased from Millipore Company; ): purchased from MichromBioresources.

[0033] 2) Main reagents: deionized water comes from MillliQ RG ultrapure water system; chromatographic grade acetonitrile, formic acid and methanol are produced by Fisher; ammonium iodoacetamide (IAA), ammonium bicarbonate and dithiothreitol (DTT) are from Merck Purchased from the company; sequencing-grade trypsin and porcine myelin protein were purchased from Sigma; tandem mass marker (TMT) labeling kit was purchased from Thermo Fisher.

[0034]3) Animals: Female Lewis rats (body weight 250-250 g) were purchased from Beijing Institute of Medical Experimental Animals and raised in a standard fe...

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Abstract

The invention relates to application of a protein marker of multiple sclerosis, specifically to application of an associative part selectively combined to one or more proteins in the preparation of a reagent for diagnosing multiple sclerosis diseases. The one or more proteins are selected from angiotensinogen, complement C9, serine secreted protein 1, fatty acid binding protein 9, neutrophil collagenase, hyaluronic acid proteoglycan connexin 1 and oncomodulin. Specifically, the invention relates to application of a multiple sclerosis diagnosis-related protein marker obtained by experimental allergic encephalomyelitis rat model and mass spectrometry.

Description

technical field [0001] The present invention relates to the use of protein markers for multiple sclerosis. Specifically, the present invention relates to the use of protein markers related to the diagnosis of multiple sclerosis obtained from an experimental allergic encephalomyelitis rat model and mass spectrometry analysis. Background technique [0002] Significance of urinary proteomics in kidney disease research: [0003] Unlike blood, which has the ability to maintain homeostasis, urine is more prone to changes, which reflect changes in body functions, that is, urine is an important source of biomarkers, see Youhe G.Can urine be the goldmine for biomarker discovery ? .Sci China Life Sci 2013; 43. [0004] Urine can often show the function of the urinary system to a certain extent, and it is convenient and simple to use urine changes to diagnose kidney diseases. The functional changes of the urinary system are not easy to detect, and the current diagnostic methods mai...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N33/6848G01N2800/34G01N2800/52G01N2800/60
Inventor 高友鹤赵敏迪李逊斗
Owner BEIJING NORMAL UNIVERSITY
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