A kind of low-alkali gel-encapsulated microbial self-healing agent and its application

A microbial and self-healing technology, applied in the biological field, can solve the problems of limited slag or ceramsite loading, limited repair efficiency, strength loss, etc., to save maintenance funds, improve repair efficiency, and good compatibility.

Active Publication Date: 2019-11-01
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But on the one hand, due to the low strength of slag or ceramsite, its introduction will cause strength loss to the cement-based material matrix; on the other hand, the loading capacity of slag or ceramsite is limited, which limits the repair efficiency

Method used

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  • A kind of low-alkali gel-encapsulated microbial self-healing agent and its application
  • A kind of low-alkali gel-encapsulated microbial self-healing agent and its application
  • A kind of low-alkali gel-encapsulated microbial self-healing agent and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A low-alkali gel-encapsulated microbial self-healing agent is prepared by the following method. Sporosarcina pasteurii is inoculated in the basic medium, and each liter of the culture medium contains 3g of beef extract, 5g of peptone and 18g of urea , shake culture at 37°C on a water bath shaker at a speed of 100rpm. After 21 days, centrifuge the obtained culture liquid at a speed of 4000rpm on a centrifuge for 10min, remove the supernatant and collect the spore liquid with deionized water to concentrate the spore The final concentration of the solution was controlled at 2×10 9 cell / ml. Prepare beef extract and peptone mass ratio of 1:1.6 nutritional ingredients, and sulfoaluminate cement and silica fume mass ratio of 1:0.2 low-alkali gelling medium, then 40g of nutritional ingredients, 240g of low-alkali gelling medium and Mix 100g of water and stir for 5min, then mix it evenly with the concentrated spore liquid at a volume ratio of 150:1, place it at 20°C and 100% re...

Embodiment 2

[0030] A low-alkali gel-encapsulated microbial self-healing agent, prepared by the following method, inoculating Sporosarcina pasteurii in the basal medium, and each liter of the culture solution contains 2.4g of beef extract, 4g of peptone and urea 16g, shake culture at 25°C on a water bath shaker at a speed of 150rpm. After 14 days, centrifuge the obtained culture liquid on a centrifuge at a speed of 3000rpm for 20min, remove the supernatant and collect the spore liquid with deionized water to concentrate the spores The final concentration of the bacterial solution was controlled at 2×10 8 cell / ml. Prepare beef extract and peptone mass ratio of 1:2 nutritional ingredients, and sulfoaluminate cement and silica fume mass ratio of 1:0.1 low-alkali gelling medium, then 36g of nutritional ingredients, 220g of low-alkali gelling medium and Mix 100g of water and stir for 3 minutes, then mix it evenly with the concentrated spore liquid at a volume ratio of 100:1, place it at 20°C a...

Embodiment 3

[0032] A low-alkali gel-encapsulated microbial self-healing agent is prepared by the following method: Sporosarcina pasteurii is inoculated in the basal medium, and each liter of the culture solution contains 3.6g of beef extract, 6g of peptone and urea 20g, shake culture at 30°C on a water bath shaker at a speed of 120rpm. After 28 days, centrifuge the obtained culture liquid on a centrifuge at a speed of 4000rpm for 15min, remove the supernatant and collect the spore liquid with deionized water to concentrate the spores The final concentration of the bacterial solution was controlled at 2×10 10 cell / ml. Prepare beef extract and peptone mass ratio of 1:1.8 nutritional ingredients, and sulfoaluminate cement and silica fume mass ratio of 1:0.15 low-alkali gelling medium, then 40g of nutritional ingredients, 230g of low-alkali gelling medium and Mix 100g of water and stir for 5min, then mix it evenly with the concentrated spore liquid at a volume ratio of 100:1, place it at 22°...

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Abstract

The invention relates to a low-alkali gel encapsulated microbial self-healing agent and application thereof. The self-healing agent comprises a component A and a component B, wherein the component A is obtained by mixing and stirring a nutritional ingredient, a low-alkali gel medium and water, uniformly mixing a mixed solution and a concentrated bacillus solution, standing for hardening, and grinding to form particles; the component B is a mixture of urea and anhydrous calcium nitrate. Dry-mixed cement mortar with the added microbial self-healing agent is used for self-healing of gaps of cement, bacterial cells can be effectively protected, the ore yield is increased, high compatibility with a cement base material is achieved, a produced healing medium is natural carbonate, and has high durability, volume stability and environment friendliness, maintenance funds can be greatly saved by a self-healing characteristic, meanwhile, the loading capacity can be remarkably improved, moreover, high compatibility with the cement base material is achieved, the influence on own performance of the cement base material can be avoided, and the self-healing efficiency can also be greatly improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a low-alkali gel-encapsulated microbial self-healing agent and its application. Background technique [0002] Biomineralization is a common phenomenon in nature, and microbial mineralization is a typical representative of it. When the environment meets certain physical and chemical conditions, microorganisms can actively or passively convert soluble ions into solid-phase minerals, which can selectively fill or bond permeable pores or cracks. It is used in petroleum engineering, geology Applications in engineering and civil engineering have attracted widespread attention. Nearly two-thirds of the more than 30 biominerals discovered are calcium ores, of which calcite is the most important. As a stable mineral, calcite produced by biomineralization can be used for self-healing of artificial stone materials such as cement-based materials. Because of its mild reaction condi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C04B28/06
CPCC04B28/06C04B2111/343C04B24/14C04B24/126C04B22/085C04B2103/0001
Inventor 徐晶王彬彬王先志左俊卿
Owner TONGJI UNIV
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