Sequential extraction process for bovine collagen peptides

Abstract

The invention provides a sequential extraction process for bovine collagen peptides, which comprises the following steps: performing primary extraction by adopting ultrasonic waves; performing solid-liquid separation to obtain filtrate and filter residue; performing primary enzymatic hydrolysis to obtain a primary enzymatic hydrolysate; performing enzyme deactivation, separating undissolved substances, and removing impurities to obtain a primary refined solution; performing concentration treatment; performing sterilization and drying to obtain primary protein peptide powder; performing secondary enzymatic hydrolysis to obtain a secondary enzymatic hydrolysate; performing concentration treatment; performing sterilization and drying to obtain secondary protein peptide powder. According to the sequential extraction process for bovine collagen peptides, the primary extraction is performed in an alkalescence environment with the pH value being 7-8, the extraction efficiency is improved under a 0-0.04 Mpa micro-high-pressure environment through matching of the operation temperature being 100-105 DEG C, the collagen peptide purity and the molecule integrity are ensured, and the protein utilization ratio is relatively high; the extraction efficiency (production efficiency) is improved through the combination of ultrasonic wave extraction, and acids, alkalies and other chemical reagents are avoided from use and emission, so that the process is friendly to the environment and does not pollute the environment.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a fractional extraction process of bovine collagen peptides. Background technique [0002] The current collagen extraction mainly includes water extraction, alkaline extraction, acid extraction and enzymatic extraction, and each method has its advantages and disadvantages. The water extraction method extracts the treated raw materials in hot water to slowly dissolve the collagen. The water temperature is generally controlled at 60-70°C. The advantages are that the collagen is relatively pure, the molecular weight is relatively uniform, and the collagen structure is complete. The disadvantages are: It takes a long time and the yield is low. The extraction time including pretreatment ranges from 10-60 days, the protein yield is between 30%-60%, and the water consumption is large. [0003] Alkaline extraction method can control a certain temperature under alkaline conditions...

AI Technical Summary

Problems solved by technology

The water extraction method is to extract the treated raw materials in hot water to slowly dissolve the collagen. The water temperature is generally controlled at 60-70°C. The advantages are that the collagen is relatively pure, the molecular weight is relatively uniform, and the collagen structure is complete. The disadvantages are: It takes a long time and the yield is low, the extraction time including pretreatment ranges from 10-60 days, the protein yield is between 30%-60%, and the water consumption is large

[0003] Alkaline extraction method can control a certain temperature under alkaline conditions to dissolve collagen, which is faster than water extraction, the time is shortened to 1-3 days, and the protein yield is increased to 50%-70%. The disadvantage is that the collagen is not pure , the miscellaneous protein is more dissolved, and the amino acid is seriously damaged, the molecular weight is not uniform and the ash content is high, and the environment is polluted

[0004] The acid method is to dissolve collagen eggs under acidic conditions and control a certain temperature. The extraction time is between water extraction and alkaline extraction, 2-5 days. The collagen is relatively pure, the amino acid is less destroyed, and the molecular structure remains intact. The disadvantage is that the extraction Long cycle, high ash content, environmental pollution

[0005] Enzymatic extraction is to control a certain temperature and PH, use protease to enzymatically hydrolyze raw materials, and extract collagen after impurity removal and fine filtration. The cycle is short, 1-24h ends, the protein yield is high, reaching 80%-90%, and the amino acid is less damaged. And the environment is friendly, the disadvantage is that the molecular weight distribution is wide, the cost is high, and there are more miscellaneous proteins