Method and reagent for detecting small and dense low-density lipoprotein cholesterol in sample

Abstract

The invention discloses a method and reagent for detecting small and dense low-density lipoprotein cholesterol (sdLDL-C) in a sample. The method is characterized by comprising the steps of (1) eliminating cholesterol, except the sdLDL-C, in lipoproteins in the sample first in the presence of cholesterol esterase and an ion selective agent; and (2) conducting quantitative detection on remaining sdLDL-C obtained after treatment in step (1). The ion selective agent is used to selectively eliminate lipoproteins other than the sdLDL-C, so as to detect the sdLDL-C.

Description

Technical field

[0001] The invention relates to the field of biotechnology, in particular to a method and reagent for measuring small and dense low-density lipoprotein cholesterol in a sample. Background technique

[0002] As we all know, according to the classification of lipoproteins, lipoproteins can be divided into chylomicrons (CM), very low density lipoprotein (VLDL), intermediate density lipoprotein (IDL), low density lipoprotein (LDL) and high density lipoprotein ( HDL). Clinically, the cholesterol content of various lipoproteins is usually measured to guide diagnosis. Among them, low-density lipoprotein cholesterol is a risk factor for atherosclerosis and has been widely used in clinical testing. However, more and more studies have shown that low-density lipoproteins are heterogeneous. Some of the denser and smaller parts are called small and dense low-density lipoproteins (sdLDL), and the other part is less dense and larger. Some are called large and light low-densit...

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