Protein chip stabilizing agent as well as preparation method and application thereof

A protein chip and stabilizer technology, which is applied in the field of protein chip stabilizer and its preparation, can solve the problems of short storage period, poor stability, low protein concentration, etc., and achieve the effects of extending storage period, saving costs and improving stability

Pending Publication Date: 2017-12-08
中山和芯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in order to reduce errors and facilitate inspection personnel, they are generally supplied in the form of protein chips, but the protein of this protein chip is attached to the chip in the form of a solid phase, and the protein concentration is low, the stability is poor, and the shelf life is short.
At present, there are glycerol-blocked protein chips in the market to extend the shelf life of protein chips, but the results are not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In this embodiment, the protein chip stabilizer includes the following components in the PBS buffer system based on the total mass of the protein chip stabilizer: 4% sucrose, 1% bovine serum white Protein, 0.05% Tween-20, 5% trehalose and 0.05% biological preservative Proclin 300, wherein the PBS buffer system is 1×PBS buffer, and its pH value is 7.2-7.4;

[0030] Add sucrose, bovine serum albumin, Tween-20, trehalose and biological preservative Proclin 300 into the PBS buffer system and mix evenly to obtain the protein chip stabilizer.

[0031] The accelerated test was carried out until the 8th day. During the entire accelerated test, the effect after the accelerated test every day was consistent with that prepared on the day, and the minimum detection limit was lower than 0.01ng / ml. Linear R 2 ﹥0.995, CV﹤10%.

Embodiment 2

[0033] In this embodiment, the protein chip stabilizer includes the following components in the PBS buffer system based on the total mass of the protein chip stabilizer: 3.5% sucrose, 1.5% bovine serum white Protein, 0.06% Tween-20, 4.5% trehalose and 0.03% biological preservative Proclin300, wherein the PBS buffer system is 1×PBS buffer, and its pH value is 7.2-7.4;

[0034] Add sucrose, bovine serum albumin, Tween-20, trehalose and biological preservative Proclin 300 into the PBS buffer system and mix evenly to obtain the protein chip stabilizer.

[0035]The accelerated test was carried out until the 8th day. During the entire accelerated test process in the first 6 days, the effect after the accelerated test every day was consistent with that prepared on the day, and the minimum detection limit was lower than 0.01ng / mL. Linear R 2 ﹥0.995, CV﹤10%. From the 7th day, the experimental results were slightly different from the effects prepared on that day, the current signal va...

Embodiment 3

[0037] In this embodiment, the protein chip stabilizer includes the following components in the PBS buffer system based on the total mass of the protein chip stabilizer: 1% sucrose, 3% bovine serum albumin Protein, 0.1% Tween-20, 8% trehalose and 0.01% biological preservative Proclin 300, wherein the PBS buffer system is 1×PBS buffer, and its pH value is 7.2-7.4;

[0038] Add sucrose, bovine serum albumin, Tween-20, trehalose and biological preservative Proclin 300 into the PBS buffer system and mix evenly to obtain the protein chip stabilizer.

[0039] The accelerated test was carried out until the 8th day. During the entire accelerated test in the first 4 days, the effect of the daily accelerated test was consistent with that prepared on the day, and the minimum detection limit was lower than 0.015ng / mL (bovine serum albumin increased, and the background increased , increased detection limit). Linear R 2 ﹥0.995, CV﹤10%. From the 5th day, the experimental results were slig...

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Abstract

The invention provides a protein chip stabilizing agent as well as a preparation method and application thereof. The protein chip stabilizing agent is prepared from saccharose, bovine serum albumin, Tween-20, trehalose and a biological preservative Proclin 300 in a PBS buffer system. The protein chip stabilizing agent prepared by the preparation method is capable of prolonging the protein immune activity storage life and reducing the cost, is safe and effective and contains no other chemical product; the saccharose and the trehalose are remarkably in synergistic effect, the stability of each of the saccharose and the trehalose can be respectively coordinated, and the stability of a whole protein chip can be more effectively increased; the protein immune activity of the protein chip in a kit during the storage life cannot be changed; a formula is simple, the cost is low, the application range is wide, no potential safety hazard exists, and good stabilizing effect and a wide application prospect are obtained.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and relates to a protein chip stabilizer and its preparation method and application. Background technique [0002] Biochip technology is a high-throughput parallel detection technology produced at the end of the 20th century. Once it was produced, it showed great advantages. The protein microarray has also developed rapidly in recent years, and has been applied to the detection of pathogens and allergens of TORCH infection. At present, some studies have used the chip method to detect 15 kinds of autoantibodies, and found that the detection sensitivity and specificity of the protein chip are comparable to the ELISA experiment. There are also studies using the chip to detect autoantibodies in the diluted serum of 18 antigens, and the specificity and sensitivity It is also very high, and can reach the detection limit of 40fg. However, the protein chips on the market have poor stabi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803G01N2333/4704
Inventor 李逢辉严巧
Owner 中山和芯生物技术有限公司
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