Protein chip stabilizing agent as well as preparation method and application thereof

Abstract

The invention provides a protein chip stabilizing agent as well as a preparation method and application thereof. The protein chip stabilizing agent is prepared from saccharose, bovine serum albumin, Tween-20, trehalose and a biological preservative Proclin 300 in a PBS buffer system. The protein chip stabilizing agent prepared by the preparation method is capable of prolonging the protein immune activity storage life and reducing the cost, is safe and effective and contains no other chemical product; the saccharose and the trehalose are remarkably in synergistic effect, the stability of each of the saccharose and the trehalose can be respectively coordinated, and the stability of a whole protein chip can be more effectively increased; the protein immune activity of the protein chip in a kit during the storage life cannot be changed; a formula is simple, the cost is low, the application range is wide, no potential safety hazard exists, and good stabilizing effect and a wide application prospect are obtained.

Description

technical field

[0001] The invention belongs to the technical field of biological preparations, and relates to a protein chip stabilizer and its preparation method and application. Background technique

[0002] Biochip technology is a high-throughput parallel detection technology produced at the end of the 20th century. Once it was produced, it showed great advantages. The protein microarray has also developed rapidly in recent years, and has been applied to the detection of pathogens and allergens of TORCH infection. At present, some studies have used the chip method to detect 15 kinds of autoantibodies, and found that the detection sensitivity and specificity of the protein chip are comparable to the ELISA experiment. There are also studies using the chip to detect autoantibodies in the diluted serum of 18 antigens, and the specificity and sensitivity It is also very high, and can reach the detection limit of 40fg. However, the protein chips on the market have poor stabi...