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a supercritical co 2 Method for extracting lychee grass polysaccharide by fluid extraction technique

An extraction technology, the technology of lychee grass, is applied in the field of supercritical CO2 fluid extraction technology to extract polysaccharides from lychee grass, which can solve the problems of low extraction rate, destruction of polysaccharide molecular structure, and high cost, so as to achieve improved extraction rate, simple operation, and improved inhibition The effect of bacterial activity

Active Publication Date: 2019-08-27
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the commonly used technology for extracting polysaccharides is the traditional water extraction and alcohol precipitation method. The disadvantage of this method is that it will destroy the structure of polysaccharides and the extraction rate is low; the disadvantage of enzymatic hydrolysis is that the cost is high; the disadvantage of ultrasonic assisted solution extraction is that it will Destroying the molecular structure of polysaccharides, the extraction rate is low

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  • a supercritical co <sub>2</sub> Method for extracting lychee grass polysaccharide by fluid extraction technique
  • a supercritical co <sub>2</sub> Method for extracting lychee grass polysaccharide by fluid extraction technique

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Embodiment 1

[0021] A supercritical CO 2 The method for extracting polysaccharides from lychee grass by fluid extraction technology comprises the following steps:

[0022] A. Wash the fresh leaves of lychee grass, remove impurities, and crush to obtain broken leaves after natural drying, with a particle size of ≤1mm;

[0023] B. Pour the crushed leaves into the sodium chloride solution and soak for 6 hours, take them out, dry them at room temperature and crush them again, the particle size is 0.5-5nm;

[0024] C. Using supercritical CO 2 The fluid is extracted, and the specific parameters are: pressure 25MPa, temperature 40°C, analytical pressure 3.5MPa, extraction time 2.5h;

[0025] D, the extracted lychee grass particles are finely pulverized, and the particle size is <0.5nm;

[0026] E. After cold water leaching, filter and carry out rotary evaporation at 55°C;

[0027] F, adopt 75% ethanol to carry out precipitation repeatedly;

[0028] G. The precipitate is vacuum-dried and then...

Embodiment 2

[0034] A supercritical CO 2 The method for extracting polysaccharides from lychee grass by fluid extraction technology comprises the following steps:

[0035] A. Wash the fresh leaves of lychee grass, remove impurities, and crush to obtain broken leaves after natural drying, with a particle size of ≤1mm;

[0036] B. Pour the crushed leaves into the sodium chloride solution and soak for 8 hours, take them out, dry them at room temperature and crush them again, the particle size is 0.5-5nm;

[0037] C. Using supercritical CO 2 The fluid is extracted, and the specific parameters are: pressure 20MPa, temperature 45°C, analytical pressure 3MPa, extraction time 4h;

[0038] D, the extracted lychee grass particles are finely pulverized, and the particle size is <0.5nm;

[0039] E. After cold water leaching, filter and perform rotary evaporation at 40°C;

[0040] F, adopt 80% ethanol to carry out precipitation repeatedly;

[0041] G. The precipitate is vacuum-dried and then pulve...

Embodiment 3

[0047] A supercritical CO 2 The method for extracting polysaccharides from lychee grass by fluid extraction technology comprises the following steps:

[0048] A. Wash the fresh leaves of lychee grass, remove impurities, and crush to obtain broken leaves after natural drying, with a particle size of ≤1mm;

[0049] B. Pour the crushed leaves into the sodium chloride solution and soak for 4 hours, take them out, dry them at room temperature and crush them again, the particle size is 0.5-5nm;

[0050] C. Using supercritical CO 2 The fluid is extracted, and the specific parameters are: pressure 30MPa, temperature 30°C, analytical pressure 5MPa, extraction time 1h;

[0051] D, the extracted lychee grass particles are finely pulverized, and the particle size is <0.5nm;

[0052] E. After cold water leaching, filter and perform rotary evaporation at 60°C;

[0053] F, adopt 70% ethanol to carry out precipitation repeatedly;

[0054] G. The precipitate is vacuum-dried and then pulve...

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Abstract

The invention discloses a supercritical CO 2 The method for extracting lychee grass polysaccharides by fluid extraction technology comprises the following steps: drying the fresh leaves of lychee grass successively, pulverizing, soaking, pulverizing again, supercritical CO 2 Fluid extraction, fine crushing, cold water leaching, filtration, alcohol precipitation, vacuum drying, and finally the crude polysaccharide is obtained; the extraction method of the present invention uses supercritical CO 2 Litchi grass polysaccharides are prepared by fluid extraction technology, which can retain almost all the activity of the polysaccharides without residue and is easy to operate; at the same time, soaking in sodium chloride solution before extraction is beneficial to improve the extraction rate of polysaccharides, adding a small amount of fresh aloe vera juice , has a synergistic effect with lychee grass polysaccharides, which can significantly improve the antibacterial activity of lychee grass polysaccharides.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a supercritical CO 2 A method for extracting lychee grass polysaccharides by fluid extraction technology. Background technique [0002] Lychee grass can be used as a whole herb, which has the characteristics of clearing away heat, detoxification, cooling blood and diuresis, especially anti-inflammatory effect. It contains high psyllogenin, ragragynin, eupillin, flavone glycosides (nepitrin), 4-hydroxyphenyl lactic acid (4-hydroxyphenyl lactic acid), caffeic acid and so on. Polysaccharides have the functions of emulsifying and regulating various physiological functions of the human body, but little attention has been paid to natural polysaccharides from lychee grass. In this study, supercritical CO 2 Fluid extraction technology extracts polysaccharides from lychee grass, and optimizes a new preparation process to ensure high extraction rate of polysaccharides from...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02P20/54
Inventor 杨立娜崔士坤刘贺马涛朱丹实张红运何余堂蔡文琪
Owner BOHAI UNIV