Herba saponariae officinalis extract and cosmetic containing herba saponariae officinalis extract
A technology for cosmetics and extracts, which is applied in the directions of medical preparations, cosmetics, and cosmetic preparations containing active ingredients, and can solve problems such as the inability to guarantee the quality of cosmetic products and the lack of any research on the quality of the extract
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[0036] Preparation of reference substance solution Accurately weigh an appropriate amount of saponin reference substance, add methanol to make a solution containing 180 μg of saponin per 1 ml.
[0037] Preparation of the test solution: Take 30 mg of the extract of Lithium japonica, accurately weighed, add methanol to dissolve, transfer to a 100ml measuring bottle, add methanol to the mark, shake well, filter, and take the subsequent filtrate as the test solution.
[0038] Sample Determination Method Precisely draw 20 μl of the test solution and reference solution, inject and measure according to the chromatographic conditions, and calculate with the external standard one-point method.
[0039] Saponin detection
[0040] Entrust ICAS Ingall Testing and Certification Group for testing.
[0041] experiment method:
[0042] Multiple skin irritation tests were performed on Kunming mice: animals were randomly selected, 6 in each group, and the hair on both sides of the back and sp...
Embodiment 1
[0070] Take 1000g of dry Sapwort (Soapwort), add 8 times the amount of 80% ethanol to reflux and extract twice, each time for 1 hour, filter, combine the extracts, recover the extract under reduced pressure, add to AB-8 macroporous adsorption On the resin column, elute with water until the eluent is nearly colorless, discard the eluent, elute with 20% ethanol until the eluent is nearly colorless, discard the eluent, and elute with 50% ethanol until the eluent The liquid is nearly colorless, collect the eluent, recover ethanol from the eluent to the end, add water 0.2 times the weight of the concentrated solution to the concentrated solution, add activated carbon 0.1 times the weight of the concentrated solution, heat to 35 ° C, keep warm for 2 hours, filter, The filtrate was concentrated and dried to obtain 46.7 g of the extract of Alkaline Flower (the content of saponin is 51.4%, and the content of saponin is 9PPM).
[0071] Get 6g of Alkaline Flower Extract and 3.5g of Snow ...
Embodiment 2
[0075] Take 1000g of dried Sapwort (Soapwort), add 12 times the amount of 60% ethanol to reflux and extract 4 times, each time for 3 hours, filter, combine the extracts, recover the extract under reduced pressure, add to AB-8 macroporous adsorption On the resin column, elute with water until the eluent is nearly colorless, discard the eluent, elute with 20% ethanol until the eluent is nearly colorless, discard the eluent, and elute with 50% ethanol until the eluent The liquid is nearly colorless, collect the eluent, recover ethanol from the eluent to the end, add water 0.4 times the weight of the concentrated solution to the concentrated solution, add activated carbon 0.2 times the weight of the concentrated solution, heat to 35 ° C, keep warm for 2 hours, filter, The filtrate was concentrated and dried to obtain 47.5 g of the extract of Alkalina japonica (the content of saponin is 54.6%, and the content of saponin is 8PPM).
[0076] Get 6g of Alkaline Flower Extract and 3.5g ...
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