Tai Lake reed marsh bottom sediment MC (microcystic) toxin degrading bacterium and MC-LR degrading method

A microcystin, MC-LR technology, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of low MCs content

Inactive Publication Date: 2017-12-15
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the weather turns cooler, the algae cells die and deposit on the shore, and a large amount of intracellular MCs enter the soil, but the content of MCs in the reed mud is not high after testing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Pick a ring of bacteria from the slant culture medium of strain JZ-4 and inoculate it into 50mL nutrient broth medium. -1 Shake culture for 12 hours, inoculate 30 mL of inorganic salt & MC-LR medium with a pH value of 7 according to the inoculation amount of 3% by volume fraction, at a temperature of 30 ° C, 140 r min -1 After shaking culture for 6 days, samples were taken every day to determine the content of MC-LR. The degradation rates of strain JZ-4 to MC-LR within 1-6 days were 11.03%, 42.05%, 68.25%, 79.34%, 84.85%, and 88.86%, respectively.

Embodiment 2

[0030] The difference between this example and Example 1 lies in the difference in the amount of bacteria inoculated.

[0031] Pick a ring of bacteria from the slant culture medium of strain JZ-4 and inoculate it into 50mL nutrient broth medium. -1 Shake culture for 12 hours, inoculate the bacterial solution into 30mL inorganic salt & MC-LR medium with a pH value of 7 according to the volume fraction of 3%, 5%, 7%, 9%, and 11% inoculum respectively. At 30°C, 140r·min -1Cultured with shaking for 6 days, and samples were taken to determine the content of MC-LR. When the inoculation amount is 3%, the degradation rate of MC-LR is 87.96%; when the inoculation amount is 5%, the MC-LR degradation rate is 84.06%; when the inoculation amount is 7%, the MC-LR degradation rate is 68.71% ; When the inoculation amount was 9%, the degradation rate of MC-LR was 60.43%; when the inoculation amount was 11%, the degradation rate of MC-LR was 50.44%. Therefore, the degradation effect of MC-LR...

Embodiment 3

[0033] The difference between this example and Example 1 and Example 2 lies in the difference in the initial pH value of the reaction system.

[0034] Pick a ring of bacteria from the slant medium of strain JZ-4 and insert it into 50mL nutrient broth medium. -1 Shake the culture for 12 hours, inoculate the bacterial solution into 30 mL of inorganic salt & MC-LR medium with pH values ​​of 5, 6, 7, 8, and 9 according to the inoculation amount of 5% by volume fraction, and inoculate at 30°C , 140r·min -1 Cultured with shaking for 6 days, and samples were taken to determine the content of MC-LR. When the initial pH value of the reaction system was 5, the degradation rate of MC-LR was 14.38%; when the initial pH value of the reaction system was 6, the degradation rate of MC-LR was 74.07%; when the initial pH value of the reaction system was 7, the MC-LR The degradation rate of LR was 84.06%; when the initial pH value of the reaction system was 8, the degradation rate of MC-LR was...

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Abstract

The invention discloses Tai Lake reed marsh bottom sediment MC (microcystic) toxin degrading bacterium and an MC-LR degrading method, and belongs to the technical field of environment-friendly water biological treatment. The collection number of microcystic toxin degrading bacterium JZ-4 is CGMCC No.14051, and the suggested classification name is Aeromonas salmonicida subsp.salmonicida. One strain of indigenous bacterium JZ-4 with microcystic toxin degrading ability is screened from the bottom sediment at the root of reed marsh collected from Baidu Port of Tai Lake, the MC-LR degrading rate reaches 88.86%, and the broad prospect is realized for removal of MCs from the drinking water source. A bacterium screening source is provided for degrading of MCs by microorganisms.

Description

technical field [0001] The invention belongs to the technical field of environmental protection water biological treatment, and relates to a method for screening MC-LR degrading bacteria from reed mud in Taihu Lake and using the same to degrade microcystins. Background technique [0002] Some natural water bodies in my country (such as Taihu Lake, Chaohu Lake, etc.) have been exposed to blue-green algae outbreaks in summer and autumn, causing microcystin (MCs) pollution. MCs is a toxin secreted by algae, which is toxic to aquatic organisms and humans. Studies have shown that it takes the animal liver as the main target organ, inhibits the activity of protein phospholipase in the liver, causes hyperphosphorylation of cytokeratin, and induces tumor necrosis factor and interleukin, leading to liver damage. At present, there are more than 90 isomers of MCs, such as MC-LR, MC-RR, and MC-YR, among which MC-LR has the strongest acute toxicity. The safety limit of MC-LR in water i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38C02F101/34
CPCC02F3/34C02F2101/34C02F2101/38C02F2103/007C12N1/20C12N1/205C12R2001/01
Inventor 张文艺陈泽慧董小娜郭惠娟王明新毛林强
Owner CHANGZHOU UNIV
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