A method for preparing methane from pineapple dregs or mango dregs through anaerobic fermentation

A technology of anaerobic fermentation and pineapple pomace, applied in fermentation, waste fuel and other directions, can solve problems such as the influence of gas production, increase the volume of fermentation tank, etc., and achieve the effect of reducing investment budget and operating costs

Pending Publication Date: 2017-12-15
CHENGDU DETONG ENVIROMENT ENG
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Problems solved by technology

Although this method can make the fermentation system normal, but the gas produc...
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Abstract

A method for preparing methane from pineapple dregs or mango dregs through anaerobic fermentation is provided. The method includes (1) weighing biogas liquid of a biogas project adopting kitchen waste as a raw material, adding the pineapple dregs or mango dregs into the biogas liquid, with the solid-liquid mass ratio being controlled to be 6:100, and the ammonia nitrogen concentration being controlled to be about 2000 mg/L, adjusting a pH value with ammonia water to 8.5-9.0, and performing anaerobic fermentation at a maintained fermentation temperature of 35 DEG C for 60 days to obtain an inoculum; (2) subjecting the product of the step (1) to solid liquid separation to obtain a liquid supernatant and a precipitate, controlling the ammonia nitrogen concentration of the liquid supernatant to be 1500-3300 mg/L, blowing the precipitate with a small amount of the liquid supernatant to fully disperse the precipitate, and mixing the liquid supernatant the ammonia nitrogen concentration of which is adjusted and the dispersed precipitate; and (3) adding the pineapple dregs or mango dregs into the mixture with the TS% being controlled to be 1-4%, and performing fermentation. The method does not need independent acidification pretreatment, and can ensure successful biogas fermentation under conditions of enough volume loads and no increase of the volume of a fermentation tank. The method does not need a special additive.

Application Domain

Waste based fuelFermentation

Technology Topic

BiogasSal ammoniac +7

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  • A method for preparing methane from pineapple dregs or mango dregs through anaerobic fermentation
  • A method for preparing methane from pineapple dregs or mango dregs through anaerobic fermentation
  • A method for preparing methane from pineapple dregs or mango dregs through anaerobic fermentation

Examples

  • Experimental program(1)
  • Comparison scheme(4)

Example Embodiment

[0022] Example 1
[0023] 1.1 Fermentation raw materials
[0024] 1.1.1 Pineapple residue and mango residue
[0025] In the experiment, pineapples and mangoes purchased from the market will be simply processed. The pineapple leaves and the pulp are removed, leaving only the pineapple skin. After being crushed with a food processor, it is wrapped with gauze, and the sugar water is squeezed out. The remaining residue is used as a fermentation material. After the mango is pitted and pulped, only the mango skin is left. The subsequent processing is the same as the mango, and the remaining residue is used as the fermentation raw material. 1.1.2 Inoculum
[0026] The inoculum for this test was taken from the biogas slurry in a pilot test of biogas production from a kitchen waste in Chengdu. Pineapple residue or mango residue was added to control the solid-liquid mass ratio to 6:100, the ammonia nitrogen concentration to be controlled at 2000 mg/L, and the pH value used The ammonia water was adjusted to 8.5-9.0, the fermentation temperature was maintained at 35°C, and anaerobic fermentation was performed for 60 days to obtain inoculum. The basic data is shown in Table 1:
[0027] Table 1
[0028]
[0029] 1.2 Test method
[0030] Pineapple pomace and mango pomace contain high sugar content, and it is easy to accumulate volatile acid in the early stage of fermentation, leading to severe acidification of the entire fermentation system. The concentration range of ammonia nitrogen in the reaction was set to 1000mg/L, 1200mg/L, 3000mg/L and 4000mg/L, and the methanogenesis fermentation test was carried out. The TS% concentration of fermentation in the existing research is less than 1%, which cannot meet the actual needs of the project. The TS% concentration of the test fermentation is set to three levels of 1%, 2% and 4%.
[0031] 1.2.1 Fermentation TS% concentration test
[0032] The TS% concentration of fermentation was set at three levels of 1%, 2% and 4%. In order to prevent the loss of bacterial species, the biogas slurry is centrifuged to separate the bacterial sludge and the supernatant, and the bacterial sludge is blown away with a small amount of supernatant. The addition ratio of bacterial mud to supernatant is 1:4. Do 2 replicates for each level, with an initial pH of 8.0, use tap water instead of the supernatant as a blank control. After filling the bottle, place it in an incubator at 35°C until no biogas is produced. Check the gas production and the proportion of methane Observe the growth of the bacteria with a microscope.
[0033] When the TS% concentration is 2%, the experimental results are shown in Table 2:
[0034] Table 2
[0035]
[0036] When the TS% concentration is 1%, the experimental results are shown in Table 3:
[0037] table 3
[0038]
[0039] When the TS% concentration is 4%, the experimental results are shown in Table 4:
[0040] Table 4
[0041]
[0042]
[0043] 1.2.3 Acidification pretreatment test
[0044] Anaerobic fermentation is carried out at TS%=2% and ammonia nitrogen concentration=3000mg/L. In the acidification stage, add 10ml of strain first. As for the shaker (the speed of the shaker is to ensure that the liquid is not shaken out), acidification is carried out under the condition of open and normal temperature, shaking for 10 minutes every 6h, and the number of acidification days are 0 days and 1 day respectively. , 2 days, 3 days, 4 days, 5 days. After the acidification is over, determine the volatile acid, then add the bacteria to the 50ml reaction system, plug the stopper and anaerobic fermentation, measure the daily gas production and methane percentage content during the fermentation process and observe the growth of methanogens. The anaerobic reaction time is 29 days. Do 2 repetitions for each acidification time.
[0045] It was found:
[0046] (1) For pineapple residue, when the acidification is carried out for one day, the gas production is similar to that without acidification; however, when the acidification time exceeds one day (2, 3, 4, and 5 days), The production volume dropped sharply, and the total gas production volume was only less than 70% of that without acidification;
[0047] (2) For mango pomace, after acidification, even if the acidification time is 1 day, the gas production is only 70% of that without acidification, and when the acidification time is 5 days, the gas production is only 40% of that without acidification. . The analysis items and test methods involved above
[0048] 1) Total solid content (TS%): using a fast moisture analyzer-----setting parameters are as follows: heating temperature 130℃, stop heating standard is 2mg/300s, stable temperature 40℃; TS%=1-moisture content .
[0049] 2) Ammonia nitrogen content: Kjeldahl titration method
[0050] 3) Gas production: The drainage gas collection method is adopted, that is, the volume of salt water in the measuring bottle is measured every day to obtain the daily biogas production.
[0051] 4) Percent methane content: Gas chromatographic analysis method-Shimadzu 2010 Gas Chromatographic Detector, the parameters are as follows: column oven temperature 85°C; PPZ inlet: temperature 120.0°C; pressure 34.648psi; flow rate 49.34ml /min; septum purge flow 11.3ml/nmin; TCD detector: temperature 130.0℃; practical air flow 30.00ml/min; makeup flow 2.000ml/min; column flow 38.00ml/min.

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