Colorimetric ratio-type acid-base fluorescent probe and preparation method and application thereof

A fluorescent probe and ratio-based technology, applied in the field of fluorescent probes, can solve the problems of inability to apply fluorescent probes, and achieve the effects of high product yield, mild reaction conditions and sensitive response.
CN107488446AActive Publication Date: 2017-12-19HENAN UNIVERSITY
4 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN UNIVERSITY
Publication Date
2017-12-19

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a colorimetric ratio-type acid-base fluorescent probe and a preparation method and an application thereof. The colorimetric ratio-type acid-base fluorescent probe has a conjugated structure of hemicyanine and p-hydroxy phenylacetaldehyde shown as a formula 1, a formula 2, or a formula 3. The colorimetric ratio-type acid-base fluorescent probe has good water-soluble performance, observation is carried out by naked eyes in a pH scope of 6.0-8.0, the liquid is yellow when the pH value is less than 7.0, the color is darker when the pH value is smaller, the liquid is red when the pH value is greater than 7.0, and the color is darker when the pH value is larger. The fluorescence detection test shows that 455 nm wavelength excitation is employed, in the pH scope of 6.6-8.0, the pH change and the fluorescence intensity ratio I530 nm / I557 nm change has a good linear relation, the fluorescent probe has colorimetric ratio characteristics under acid-base response, and the colorimetric ratio-type acid-base fluorescent probe has good application in autophagy process detection of tumor cells.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of fluorescent probes, in particular to a colorimetric ratio type acid-base fluorescent probe and its preparation method and application. Background technique

[0002] The fluorescent probe analysis method has the characteristics of high sensitivity, strong selectivity, real-time in situ detection and visual detection. Fluorescent probes are more advantageous in the analysis of biological samples due to the non-destructive nature of the sample during the analysis. At present, a lot of research work is being carried out around the biological imaging characteristics of fluorescent probes. Among them, for biologically active small molecules related to living organisms (active sulfur, active nitrogen, active oxygen, etc.), important ions associated with biochemical processes (Zn 2+ 、Gu 2+ , Hg 2+ , and H + / OH - etc.) and other detectors as the target of the research work of new structural fluorescent probes are bec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More