A kind of sulforaphane with anti-influenza virus effect and its preparation method and application
An anti-influenza virus and sulforaphane technology, applied in the field of broccoli extract, can solve the problems of preventing and treating influenza without sulforaphane, and achieve the effect of strong anti-influenza virus activity and good purity
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Embodiment 1
[0045] The preparation of embodiment 1 sulforaphane (I)
[0046] (1) Enzymolysis: Add KH with a concentration of 0.1M and a pH of 7.0 to broccoli seeds 2 PO 4 -K 2 HPO 4 Buffer solution, stirred at 25°C for 1.5h to carry out enzymolysis reaction, wherein, the broccoli seeds and the KH 2 PO 4 -K 2 HPO 4 The material-to-liquid ratio of the buffer is 1:30;
[0047] (2) Add ethyl acetate for extraction, centrifuge at 5500rpm for 10min, collect the supernatant, repeat 2-3 times;
[0048] (3) Rotate the supernatant obtained in step (2) to dryness at 35°C, add 10.0 mL of dimethyl sulfoxide to constant volume, filter through a 0.22 μm filter membrane, and store at -20°C for future use.
Embodiment 2
[0049] The preparation of embodiment 2 sulforaphane (II)
[0050] (1) Enzymolysis: adding Na with a concentration of 0.15M and a pH of 7.5 to the broccoli flower 2 HPO 4 - citric acid buffer solution, stirred at 30°C for 1.2h to carry out enzymolysis reaction, wherein, the broccoli flower and the Na 2 HPO 4 - The solid-to-liquid ratio of citric acid buffer is 1:40;
[0051] (2) Add petroleum ether for extraction, centrifuge at 5000rpm for 15min, collect the supernatant, repeat 2-3 times;
[0052] (3) Rotate the supernatant obtained in step (2) to dryness at 32°C, add 10.0 mL of deionized water to constant volume, filter through a 0.3 μm filter membrane, and store at -20°C for future use.
Embodiment 3
[0053] The preparation of embodiment 3 sulforaphane (III)
[0054] (1) Enzymolysis: Add a citric acid-sodium citrate buffer solution with a concentration of 0.05M and a pH of 6.0 to the broccoli stem, and stir at 35° C. for 1.0 h to carry out the enzymolysis reaction, wherein the broccoli stem and the The solid-liquid ratio of described citric acid-sodium citrate buffer solution is 1:20;
[0055] (2) add benzene to extract, centrifuge at 4000rpm for 20min, collect supernatant, repeat 2-3 times;
[0056] (3) Rotate the supernatant obtained in step (2) to dryness at 30°C, add 10.0 mL of ethanol to constant volume, filter through a 0.4 μm filter membrane, and store at -20°C for future use.
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