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Recombinant human type-III collagen modified biovalve material and preparation method thereof

A collagen and biological valve technology, which is applied in the field of recombinant human type III collagen modified biological valve material and its preparation field, can solve problems such as prone to coagulation, and achieve remarkable anticoagulation performance and low immune rejection in the human body. , the effect of high cell adhesion activity

Active Publication Date: 2020-06-23
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned problems existing in the prior art, the present invention provides a biological valve material modified with recombinant human type III collagen and a preparation method thereof, which can effectively reduce the toxic and side effects of the existing glutaraldehyde-crosslinked biological valve material, Effectively solve the problem of its prone to blood clotting

Method used

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  • Recombinant human type-III collagen modified biovalve material and preparation method thereof
  • Recombinant human type-III collagen modified biovalve material and preparation method thereof
  • Recombinant human type-III collagen modified biovalve material and preparation method thereof

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Embodiment 1

[0026] A method for preparing a biological valve material modified by recombinant human type III collagen, comprising the following steps:

[0027] (1) Collect fresh porcine pericardium, clean it, and then soak it in an aqueous glutaraldehyde solution with a volume concentration of 0.6% for 24 hours to obtain an animal-derived pericardium biomaterial crosslinked by glutaraldehyde;

[0028] (2) Wash the animal-derived pericardial biological material cross-linked by glutaraldehyde, soak it in 30 mg / ml recombinant human type III collagen aqueous solution, soak for 24 hours, then take it out and clean it with distilled water; among them, the recombinant human-derived type III collagen The amino acid sequence of type collagen is GERGAPGFRGPAGPNGIPGEKGPAGERGAP.

Embodiment 2

[0030] A method for preparing a biological valve material modified by recombinant human type III collagen, comprising the following steps:

[0031] (1) Collect fresh porcine pericardium, clean it, and then soak it in an aqueous glutaraldehyde solution with a volume concentration of 0.6% for 12 hours to obtain an animal-derived pericardium biomaterial crosslinked by glutaraldehyde;

[0032] (2) Wash the animal-derived pericardial biological material cross-linked by glutaraldehyde and soak it in 15 mg / ml recombinant human type III collagen aqueous solution for 24 hours, then take it out and clean it with distilled water; among them, the recombinant human-derived type III collagen The amino acid sequence of type collagen is GERGAPGFRGPAGPNGIPGEKGPAGERGAP.

Embodiment 3

[0034] A method for preparing a biological valve material modified by recombinant human type III collagen, comprising the following steps:

[0035] (1) Collect fresh porcine pericardium, clean it, and then soak it in an aqueous glutaraldehyde solution with a volume concentration of 0.6% for 12 hours to obtain an animal-derived pericardium biomaterial crosslinked by glutaraldehyde;

[0036] (2) Wash the animal-derived pericardial biological material cross-linked by glutaraldehyde and soak it in 30 mg / ml recombinant human type III collagen aqueous solution for 1 hour, then take it out and clean it with distilled water; among them, the recombinant human-derived type III collagen The amino acid sequence of type collagen is GERGAPGFRGPAGPNGIPGEKGPAGERGAP;

[0037] (3) Soak the material obtained in step (2) in 50mmol / L sodium cyanoborohydride (NaCNBH 3 ) in PBS solution, soaked for 12 hours, and then washed with distilled water.

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Abstract

The invention provides a recombinant human type-III collagen modified biovalve material and a preparation method thereof. The preparation method comprises the following steps: soaking a glutaraldehydecross-linked biovalve material into a human type-III collagen solution, adjusting the pH value of the solution to 3-8, controlling the soaking time to 1-24 hours, taking out the material, and cleaning the material with deionized water, so as to obtain the recombinant human type-III collagen modified biovalve material. By adopting the biovalve material provided by the invention, toxic and side functions of the glutaraldehyde cross-linked biovalve material can be effectively reduced, and a problem that the glutaraldehyde cross-linked biovalve material is liable to cause blood coagulation can beeffectively solved.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a biological valve material modified by recombinant human type III collagen and a preparation method thereof. Background technique [0002] Heart valve disease is a common disease of valve degeneration, which is anatomically manifested by narrowing of blood pathways or valve insufficiency. Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. [0003] Biological heart valves refer to a class of biomedical materials used to replace human diseased heart valves. Biological heart valves are gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/24A61L27/50
CPCA61L27/24A61L27/3604A61L27/3625A61L27/3687A61L27/50A61L2400/02A61L2430/20A61L2430/40A61L2300/802
Inventor 杨立王云兵陆路杨霞张兴栋
Owner SICHUAN UNIV
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