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Sulforaphane with anti-influenza virus effect, and preparation method and application thereof

An anti-influenza virus and sulforaphane technology, applied in the field of broccoli extract, can solve the problems of preventing and treating influenza without sulforaphane, and achieve the effect of strong anti-influenza virus activity and good purity

Active Publication Date: 2017-12-22
北京蔬行苑科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no technical implications for the use of sulforaphane in the prevention and treatment of influenza

Method used

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  • Sulforaphane with anti-influenza virus effect, and preparation method and application thereof
  • Sulforaphane with anti-influenza virus effect, and preparation method and application thereof
  • Sulforaphane with anti-influenza virus effect, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 sulforaphane (I)

[0046] (1) Enzymolysis: Add KH with a concentration of 0.1M and a pH of 7.0 to broccoli seeds 2 PO 4 -K 2 HPO 4 Buffer solution, stirred at 25°C for 1.5h to carry out enzymolysis reaction, wherein, the broccoli seeds and the KH 2 PO 4 -K 2 HPO 4 The material-to-liquid ratio of the buffer is 1:30;

[0047] (2) Add ethyl acetate for extraction, centrifuge at 5500rpm for 10min, collect the supernatant, repeat 2-3 times;

[0048] (3) Rotate the supernatant obtained in step (2) to dryness at 35°C, add 10.0 mL of dimethyl sulfoxide to constant volume, filter through a 0.22 μm filter membrane, and store at -20°C for future use.

Embodiment 2

[0049] The preparation of embodiment 2 sulforaphane (II)

[0050] (1) Enzymolysis: adding Na with a concentration of 0.15M and a pH of 7.5 to the broccoli flower 2 HPO 4 - citric acid buffer solution, stirred at 30°C for 1.2h to carry out enzymolysis reaction, wherein, the broccoli flower and the Na 2 HPO 4 - The solid-to-liquid ratio of citric acid buffer is 1:40;

[0051] (2) Add petroleum ether for extraction, centrifuge at 5000rpm for 15min, collect the supernatant, repeat 2-3 times;

[0052] (3) Rotate the supernatant obtained in step (2) to dryness at 32°C, add 10.0 mL of deionized water to constant volume, filter through a 0.3 μm filter membrane, and store at -20°C for future use.

Embodiment 3

[0053] The preparation of embodiment 3 sulforaphane (III)

[0054] (1) Enzymolysis: Add a citric acid-sodium citrate buffer solution with a concentration of 0.05M and a pH of 6.0 to the broccoli stem, and stir at 35° C. for 1.0 h to carry out the enzymolysis reaction, wherein the broccoli stem and the The solid-liquid ratio of described citric acid-sodium citrate buffer solution is 1:20;

[0055] (2) add benzene to extract, centrifuge at 4000rpm for 20min, collect supernatant, repeat 2-3 times;

[0056] (3) Rotate the supernatant obtained in step (2) to dryness at 30°C, add 10.0 mL of ethanol to constant volume, filter through a 0.4 μm filter membrane, and store at -20°C for future use.

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Abstract

The invention provides a novel purpose of sulforaphane as anti-influenza virus medicine. The sulforaphane has high anti-influenza virus activity in the low-concentration range; no obvious cytotoxicity exists; the quantity of influenza virus infected cells can be increased; and the potential value of being used as an active ingredient of the anti-influenza virus medicine is realized.

Description

technical field [0001] The invention belongs to the field of broccoli extracts, and relates to sulforaphane with anti-influenza virus effect and its preparation method and application. Background technique [0002] Influenza, referred to as influenza, is an acute respiratory infectious disease caused by influenza virus, which is common in children, while infants, children and the elderly with underlying diseases often have a poor prognosis. Influenza virus is an RNA virus belonging to the Orthomyxoviridae family, which can infect a variety of hosts such as humans, poultry, and pigs, causing acute upper respiratory tract infections and even threatening life in severe cases. The World Health Organization (WHO) estimates that about 1 billion people worldwide are infected with influenza virus every year, and about 500,000 of them die from it. [0003] Influenza viruses are divided into four types: Influenza A virus, Influenza B virus, Influenza C virus and Influenza D virus. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/26A61P31/16C07C331/20C12P13/00
CPCA61K31/26C07C331/20C12P13/00
Inventor 李占省刘玉梅方智远杨丽梅庄木张扬勇吕红豪
Owner 北京蔬行苑科技有限公司
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