Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for inactivating virus in protein solution by ultraviolet irradiation

A technology of protein solution and ultraviolet rays, which is applied in disinfection, irradiation, sanitary equipment for toilets, etc., can solve the problems of effective protein component destruction and achieve good inactivation effect

Active Publication Date: 2021-02-02
广州白云山拜迪生物医药有限公司
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing method for inactivating viruses in protein solutions by ultraviolet irradiation often destroys effective protein components in protein solutions while inactivating viruses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for inactivating virus in protein solution by ultraviolet irradiation
  • A method for inactivating virus in protein solution by ultraviolet irradiation
  • A method for inactivating virus in protein solution by ultraviolet irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Flow type protein solution ultraviolet inactivation instrument

[0046] Such as Figure 1 ~ Figure 3 As shown, the flow type protein solution ultraviolet inactivation instrument used in the present invention comprises a cylinder 1, the cylinder is a dark box, the inside of the cylinder is coated with a reflective coating 2, and the side of the cylinder is provided with an openable movable panel 10 , and the movable panel is provided with a visual window, which is convenient for observing the situation in the cylinder, and through this window, the probe of the ultraviolet intensity meter can be inserted into the cylinder to measure the intensity of ultraviolet rays, or the thermometer can be inserted into the cylinder to measure the temperature.

[0047] Two sets of UV lamp groups 3 arranged symmetrically up and down are fixed in the cylinder body 1. The purpose of symmetrical placement is to ensure that the protein solution can be irradiated with ultraviolet ...

Embodiment 2

[0053] Example 2: Flow type protein solution ultraviolet inactivation instrument

[0054] Such as Figure 4~5 As shown, in the flow-type protein solution ultraviolet inactivation instrument of this embodiment, the protein solution flow tube 5 is in a spiral shape, and what is selected is a quartz glass tube. The input end of the protein solution flow pipe 5 passes through the lower end of the side panel of the cylinder body 1 and is arranged outside the cylinder body, and the output end of the protein solution flow pipe 5 passes through the upper end of the side panel of the cylinder body 1 and is arranged outside the cylinder body, and the protein solution flows The input end and output end of the tube 5 are respectively located at the two ends of the barrel. The structure of other parts of the flow-type protein solution ultraviolet inactivation instrument of this embodiment is the same as that of Embodiment 1.

[0055] In other embodiments, the protein solution flow pipe c...

Embodiment 3

[0056] Example 3: Inactivation verification of porcine parvovirus (PPV) in fibrinogen

[0057] The cultivation of porcine parvovirus (PPV): add the porcine parvovirus (PPV) liquid into a monolayer or overgrow 90% porcine testicular cells (ST cells, Figure 6 ), after 2 hours of adsorption, discard the virus liquid, add DMEM medium containing 2% fetal bovine serum (FBS) to culture the virus, and collect the virus liquid when 3 / 4 cells have lesions after about 72 hours. The culture medium containing the virus and host cells was placed at -20°C and repeatedly frozen and thawed 3 times to break up the host cells and release the virus. Then, centrifuge at 4° C. (3000 rpm, 10 min) to remove cell debris, and the supernatant is the desired virus suspension.

[0058] Determination of virus titer: 10-fold serial dilution of the virus stock solution was performed with DMEM culture solution containing 2% FBS. Take a 96-well cell culture plate covered with a monolayer of host cells, pour...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
strengthaaaaaaaaaa
concentrationaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for inactivating viruses in a protein solution by ultraviolet radiation, which is characterized in that it comprises the following steps: S1: preparing a protein solution to be treated in a closed container; Inactivation instrument connection; S3: Turn on the UV lamp group to irradiate the protein solution, and the intensity of ultraviolet radiation on the protein solution flow tube is 2.0-10.0mw / cm 2 , the irradiation time is 5s-60min, the protein solution is injected into the flow tube, and the protein solution after the inactivated virus is collected after the irradiation is completed. In the present invention, the protein solution is fully mixed in a specific device, and the ultraviolet rays can inactivate microorganisms such as viruses / bacteria, thereby achieving the inactivation effect while retaining the active components of the protein in the protein solution.

Description

technical field [0001] The invention relates to a method for inactivating virus in flowing liquid, in particular to a method for inactivating virus in protein solution by ultraviolet irradiation. Background technique [0002] In the production process of protein solutions, the uncertainty of production safety is often increased due to the use of materials derived from human or animal tissues or body fluids. Therefore, in order to improve the safety of process production and to avoid the threat of pathogens in the process of using the product, product safety must be fully considered during the research and development of protein solutions. my country's "Blood Products Removal / Inactivation Virus Technical Methods and Verification Guidelines" pointed out: In order to improve the safety of blood products, the production process must have a certain ability to remove / inactivate some viruses, and there should be specific removal / inactivation in the production process. virus method....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/10A61L2/26
CPCA61L2/0047A61L2/26A61L2202/21
Inventor 张俊辉陈柯君朱晋辉万华印
Owner 广州白云山拜迪生物医药有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com