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Preparation method for stingray cartilage polysaccharide

A technology of cartilage and polysaccharides, which is applied in the field of preparation of ray cartilage polysaccharides, can solve the problems of few reports on the immunomodulatory activity of ray cartilage polysaccharides, and achieve the effects of avoiding protein denaturation, good extraction effect, and shortening the extraction time

Inactive Publication Date: 2017-12-22
浦江县欧立生物技术有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The results of in vitro activity research on S180 tumors suggest that polysaccharides of ray cartilage polysaccharides may have high anti-tumor activity and promote the immune function of the body, but there are few reports on the immunomodulatory activity of polysaccharides of ray cartilage polysaccharides

Method used

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Effect test

Embodiment 1

[0019] A method for preparing ray cartilage polysaccharides, comprising pretreatment; cell disruption; polysaccharide extraction; polysaccharide column chromatography; freeze-drying, the specific operation steps are as follows:

[0020] 1) The pretreatment steps are: take fresh stingray cartilage, dry it, crush it, pass it through a 64-mesh sieve, and degrease it with petroleum ether to obtain defatted stingray cartilage powder;

[0021] 2) Cell crushing: take stingray cartilage powder and sieve it to make a solution with a solid-to-liquid ratio of 1:72 and add an active polypeptide of 0.45% of the weight of the cartilage. The extractant was repeatedly frozen and thawed 4 times at -20°C and 37°C, 3 hours each time, gradient ultrasonic assisted freezing and thawing, the ultrasonic power was 550w, the time was 18min, and the ultrasonic power was increased by 15w every 15min until the repeated freezing and thawing was over. Centrifuge at 4500r / min for 8min to obtain crude protein...

Embodiment 2

[0026] A method for preparing ray cartilage polysaccharides, comprising pretreatment; cell disruption; polysaccharide extraction; polysaccharide column chromatography; freeze-drying, the specific preferred operating steps are as follows:

[0027] 1) The pretreatment steps are: take fresh stingray cartilage, dry it, crush it, pass through a 56-mesh sieve, degrease with petroleum ether, and obtain defatted stingray cartilage powder;

[0028] 2) Cell crushing: Take stingray cartilage powder and sieve it to make a solution with a solid-to-liquid ratio of 1:68 and add an active polypeptide of 0.45% of the cartilage weight. The extractant was repeatedly frozen and thawed 4 times at -20°C and 37°C, 3 hours each time, gradient ultrasonic assisted freezing and thawing, the ultrasonic power was 600w, the time was 18min, and the ultrasonic power was increased by 16w every 10min until the repeated freezing and thawing was over. Centrifuge at 6000r / min for 8min to obtain crude protein extr...

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Abstract

The invention discloses a preparation method for a stingray cartilage polysaccharide, and belongs to the technical field of polysaccharide extraction. Extraction steps of the cartilage polysaccharide are as follows: pretreatment; cell disruption; polysaccharide extraction; polysaccharide column chromatography; and freeze drying. According to the preparation method, active polypeptides and a repeated freeze-thaw method are utilized for disrupting cartilage cell walls, thus the yield of the polysaccharide is improved, and through separation and purification, the purity of the polysaccharide is further improved, and the extracted polysaccharide has a low molecular weight, is applied to health care products which have immunity regulating functions and drinking products, and is easily absorbed by the human body.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a preparation method of ray cartilage polysaccharide. Background technique [0002] Stingrays belong to the vertebrate phylum Chondrichthyes, subclass Plasmodontidae, and are distributed in the Yellow Sea, Bohai Sea, East China Sea, and South my country Sea in China. The main species used today are red stingrays, but there are also several other species. With the development of medical science and technology, people's research on cartilage anti-tumor is getting more and more in-depth. Among the many researches on cartilage, the research on shark cartilage is eye-catching. However, due to the late sexual maturity and long sexual cycle of sharks, and the The unrestrained hunting in the world has resulted in the shortage of cartilage resources. my country's coastal waters are vast, and its stingray resources are abundant and cheap, which will be an ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0069C08B37/0003
Inventor 李冰
Owner 浦江县欧立生物技术有限公司
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