The invention relates to an additive for preparation of cellulose and a preparation method of the cellulose. The additive is prepared from raw materials in parts by weight as follows: 10-15 parts of saccharomycetes, 10-18 parts of urea, 12-18 parts of fatty alcohol-polyoxyethylene ether, 8-12 parts of alkylphenol ethoxylates, 12-18 parts of lipase, 3-8 parts of cellolase, 3-8 parts of aspergillus niger and 3-8 parts of a hydrolytic enzyme. The additive prepared from the combined raw materials and added to fibrous raw materials for preparation of cellulose has stable performance and can shorten the fiber preparation process, prepared fibers have a uniform length, the beating degree (degree sR), the quantitation (g/m<2>), the tightness (g/cm<3>), the whiteness (%ISO), the opaqueness (%), the fracture length (km), the tearing index (mN*m<2>/g), the bursting strength index (KPa*m<2>/g), the folding strength (times by 180 degrees), the average length (mm) of fibers and the average width of the fibers (mu m) all reach the standards, and the cellulose has higher performance.