Preparation method of ecological alga biostimulant

A biostimulant and seaweed technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of incomplete extraction, achieve the effects of improving permeability, preventing crop absorption, and improving acidified soil

Inactive Publication Date: 2017-12-22
杨丽丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, seaweed extracts are extracted by enzymatic degradation and microbial fermentation, which has the problem of incomplete extraction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Preparation of crushed seaweed: crushing washed fresh kelp to obtain crushed seaweed;

[0035] 2) Preparation of saccharified seaweed: put broken seaweed and water into a stirring tank at a mass ratio of 20:80, and stir for 6 hours at 65° C. with a stirring speed of 45 r / min to obtain saccharified seaweed;

[0036] 3), preparing seaweed filtrate and seaweed residue: using a 650-mesh filter cloth press filter to press-filter the saccharified seaweed, the obtained filtrate is seaweed filtrate, and the obtained residue is seaweed residue;

[0037] 4) Preparation of seaweed fermentation product: add seaweed residue, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose into the sterilized fermentation tank according to the mass ratio of 95.5:1:0.5:1:1:1 Mix well, and adjust the water content to 60% with water to obtain a mixed medium, insert Candida utilis, the mass ratio of Candida utilis and mixed medium is 0.5:99.5, at a temperature of 3...

Embodiment 2

[0046] 1) Preparation of broken seaweed: drying the washed fresh seaweed, crushing the dried seaweed with a pulverizer to obtain broken seaweed;

[0047]2) Preparation of saccharified seaweed: put broken seaweed and water into a stirring tank at a mass ratio of 20:80, and stir for 6 hours at 65° C. with a stirring speed of 45 r / min to obtain saccharified seaweed;

[0048] 3), preparing seaweed filtrate and seaweed residue: using a 650-mesh filter cloth press filter to press-filter the saccharified seaweed, the obtained filtrate is seaweed filtrate, and the obtained residue is seaweed residue;

[0049] 4) Preparation of seaweed fermentation product: add seaweed residue, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose into the sterilized fermentation tank according to the mass ratio of 95.5:1:0.5:1:1:1 Mix well, and adjust the water content to 60% with water to obtain a mixed medium, insert Candida utilis, the mass ratio of Candida utilis and...

Embodiment 3

[0058] 1) Preparation of crushed seaweed: crushing Enteromorpha after washing, packaging and drying to obtain crushed seaweed;

[0059] 2) Preparation of saccharified seaweed: put broken seaweed and water into a stirring tank at a mass ratio of 20:80, and stir for 6 hours at 65° C. with a stirring speed of 45 r / min to obtain saccharified seaweed;

[0060] 3), preparing seaweed filtrate and seaweed residue: using a 650-mesh filter cloth press filter to press-filter the saccharified seaweed, the obtained filtrate is seaweed filtrate, and the obtained residue is seaweed residue;

[0061] 4) Preparation of seaweed fermentation product: add seaweed residue, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose into the sterilized fermentation tank according to the mass ratio of 95.5:1:0.5:1:1:1 Mix well, and adjust the water content to 60% with water to obtain a mixed medium, insert Candida utilis, the mass ratio of Candida utilis and mixed medium is ...

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PUM

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Abstract

The invention relates to a preparation method of ecological alga biostimulant. The preparation method of the ecological alga biostimulant comprises the following steps: adding algae which are completely washed and crushed into a stirring tank, adding certain water, stirring and saccharifying at certain temperature, performing pressure filtration to obtain alga filtrate and alga residue, adding the alga residue into a fermenting tank, inoculating saccharomycetes, adding certain water and then fermenting, sterilizing after fermenting for a period of time, mixing the material and the alga filtrate to obtain mixed liquid, inoculating corynebacterium ammoniagenes into the mixed liquid, and thus obtaining the ecological alga biostimulant after fermenting for a certain time. The preparation method of the ecological alga biostimulant has the effects of conditioning soil and promoting crop growth.

Description

technical field [0001] The invention relates to a preparation method of an ecological seaweed biostimulant. Background technique [0002] Seaweed contains natural stimulants that stimulate crop growth. Seaweeds that grow deep in the sea contain stress-resistant substances due to the cold and high salinity of the environment. The non-destructive extraction of these substances in seaweed is the direction of current research. [0003] At present, the extraction methods of active ingredients of seaweed mainly include chemical methods, physical methods and biological methods. The chemical method mainly uses acid, alkali and organic solvents to treat seaweed cells to digest the cells or solubilize endogenous substances. This method is simple to operate and easy to implement, and it is also the method adopted by most seaweed fertilizer manufacturers at home and abroad. However, the damage of chemical reagents to the active substance components in seaweed cells is considerable, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P19/32C12P19/02C09K17/14C12R1/72C12R1/15C09K101/00C09K109/00
CPCC12P21/00C09K17/14C09K2101/00C09K2109/00C12P19/02C12P19/32
Inventor 杨丽丽
Owner 杨丽丽
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