A rapid method for detecting morphological characteristics of vessel cells in lignocellulosic materials
A technology of lignocellulosic materials and cell morphology, which is applied in the preparation of analytical materials, test samples, measuring devices, etc., can solve the problem of inability to accurately obtain the morphological characteristic parameters of duct cells and so on.
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Examples
Embodiment 1
[0047] 1) In order to obtain the microscopic image of poplar wood, pre-sample preparation of poplar wood material:
[0048] According to the shape of lignocellulosic hardwood poplar, the cross-sectional area should be less than 10mm 2 The poplar wood was cut into a column material with a longitudinal length of 15 mm, and the block material was used as a sample for its microscopic image acquisition.
[0049] 2) The prepared sample is obtained through an optical microscope to obtain a microscopic image that can distinguish each component cell of the poplar sample:
[0050] The sample prepared in step 1) was cut into slices with a thickness of 20 μm by conventional wood sectioning method; then the slices were dyed with 1% safranin aqueous solution dye, and the staining time was 10-12 hours; Each tissue cell was dehydrated, and xylene was used to make the dehydrated tissue cells transparent, and the sections after the tissue cells became transparent were sealed with Canada gum mo...
Embodiment 2
[0066] 1) In order to obtain the microscopic image of the moso bamboo wood, the pre-sample preparation of the moso bamboo wood was carried out:
[0067] According to the shape of the lignocellulosic material Moso bamboo, cut the stem ring of Moso bamboo with a cross-sectional area greater than 10 mm into a cuboid material of 10 mm (radial direction) × 10 mm (chord direction) × 15 mm (longitudinal direction). The bulk material was used as a sample for its microscopic image acquisition.
[0068] 2) The prepared sample is obtained by an optical microscope to be able to distinguish the microscopic image of each component cell of the moso bamboo sample:
[0069] With the sample prepared in step 1), except that the section is stained with 2% safranin alcohol dye, and the staining time is 2-4 hours, other operations are carried out the same as in Example 1.
[0070] 3) Determine and process the relationship between the image pixel and the actual size ratio of the obtained microscopi...
Embodiment 3
[0084] 1) In order to obtain the microscopic image of the yellow rattan wood, the pre-sample preparation of the yellow rattan wood:
[0085] The shape of the cut rattan material sample is the same as in Example 2. The bulk material was used as a sample for its microscopic image acquisition.
[0086] 2) Obtain a microscopic image capable of distinguishing each constituent cell of the rattan material sample through an optical microscope: the acquisition procedure of the microscopic image of the rattan material is the same as in Example 2.
[0087] 3) Determine and process the relationship between the image pixel and the actual size ratio of the obtained microscopic image of the rattan wood, so as to ensure the readability of the morphological characteristics of the ductal cells in the microscopic image and the accuracy of identification and detection, specifically including:
[0088] 3.1) Determining the correlation ratio between image pixels and actual size: same as embodiment...
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Abstract
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