Developing method of melon haploid

A haploid and melon technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low melon haploid efficiency, achieve haploid plant quality, shorten test period, and increase proliferation efficiency effect

Inactive Publication Date: 2017-12-29
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of the low efficiency of melon haploid induced by melon megaspore culture, and provides a method for creating melon haploid, which is simple and efficient, and is suitable for large-scale promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of melon haploid breeding method, comprises the steps:

[0027] 1) The variety of melon is Baifukang M288. In the early morning of the day when the male flowers of the melon open, before the male flowers open, the male flowers are picked, put into waterproof paper bags, and used 60 Co gamma ray radiation 300Gy inactivation;

[0028] 2) pollinate the female flowers that were bagged and isolated the day before and opened that day with the male flower pollen in step 1), and continue to bag and isolate the female flowers after pollination;

[0029] 3) On the 12th day after pollination, pick off the pollinated ovary, clean the epidermis, soak it with 75% alcohol solution by volume on the ultra-clean workbench for 60s, and then use 0.1% by weight mercury chloride solution Soak for 10 minutes for disinfection, rinse 5 times with sterile water, dry the surface water of young fruit with sterile paper, cut the young fruit into 5mm slices, pick young embryos, and inoculate...

Embodiment 2

[0035] A method for creating a melon haploid, comprising the steps of:

[0036] 1) The variety of melon is Baifukang M288. In the early morning of the day when the male flowers of the melon open, before the male flowers open, the male flowers are picked, put into waterproof paper bags, and used 60 Co gamma ray radiation 400Gy inactivation;

[0037] 2) pollinate the female flowers that were bagged and isolated the day before and opened that day with the male flower pollen in step 1), and continue to bag and isolate the female flowers after pollination;

[0038] 3) On the 11th day after pollination, pick off the pollinated ovary, clean the epidermis, soak it with 75% alcohol solution by volume on the ultra-clean workbench for 30s, and then use 0.1% by weight mercury chloride solution Soak for 8 minutes for disinfection, rinse with sterile water for 3 times, dry the surface moisture of the young fruit with sterile paper, cut the young fruit into 3mm slices, pick the young embryo...

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Abstract

The invention belongs to the field of plant tissue culture and breeding and discloses a developing method of melon haploid. The method includes the steps of A, deactivating melon pollen; B, using pollen radiation to perform pollination on melon female flowers which bloom on the current day; C, selecting ovaries 11-12 days after the pollination, taking out immature embryos, and culturing the immature embryos in an MS culture medium; D, picking out green embryos, and culturing the green embryos in an induction culture medium; E, after buds grow, using a flow cytometer to perform ploidy identification on leaves; F, performing induction rooting on plants, which are identified as the haploid, in a rooting culture medium to obtain haploid plants. The method has the advantages that the method is applicable to domestic thin-peel melon varieties; the cultured haploid plants can be directly used as breeding materials after field screening, and the method is applicable to melon variety breeding and can accelerate melon breeding.

Description

technical field [0001] The invention relates to plant tissue culture and breeding technology, in particular to a method for creating a melon haploid. Background technique [0002] Melon is one of the important horticultural crops, and the promotion and popularization of new varieties plays a very important role in the development of melon production. Breeding high-quality, high-yield, strong stress resistance, resistance to a variety of major melon diseases, and strong adaptability is the development direction of melon breeding. However, traditional melon breeding methods have problems such as difficulty in separating offspring stably, difficulty in aggregating multiple target traits, long breeding cycle, and low breeding efficiency. It is difficult to breed breakthrough varieties only by traditional conventional breeding methods and existing germplasm resources. [0003] The haploid breeding method uses methods such as anther culture, unfertilized ovary culture, and embry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 戴照义王运强刘志雄郭凤领龚钰
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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