LncRNA composition for detecting prognosis of early esophageal cancer and kit comprising LncRNA composition

A technology for early detection of esophageal cancer, applied in the field of biomedicine, can solve the problems of incomplete prognosis, diagnosis and treatment of esophageal squamous cell carcinoma, and achieve the effects of improving detection accuracy, improving prognosis evaluation, and improving survival rate

Inactive Publication Date: 2017-12-29
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the current situation of incomplete prognosis

Method used

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  • LncRNA composition for detecting prognosis of early esophageal cancer and kit comprising LncRNA composition
  • LncRNA composition for detecting prognosis of early esophageal cancer and kit comprising LncRNA composition
  • LncRNA composition for detecting prognosis of early esophageal cancer and kit comprising LncRNA composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1. Using RT-qPCR technology to detect the differential expression of lncRNA combination in cancerous tissue and adjacent tissues of esophageal squamous cell carcinoma 1. Extraction of total RNA from esophageal squamous cell carcinoma tissue and corresponding adjacent tissues with nucleic acid purification column

[0065] Pretreatment: In a clean area with less RNase interference (all utensils are wiped with 1‰ DEPC water), the mortar needs to be sterilized at 200°C for 5 hours and cooled for 4 hours.

[0066] 2. Tissue lysis:

[0067] 1) Weigh approximately 20 mg of the isolated esophageal squamous cell carcinoma tissue and adjacent tissue samples into a mortar that has been pre-cooled with liquid nitrogen, and grind it with a pestle to powder (incomplete grinding will seriously affect the RNA Yield); 2) Add 700μl of QIAzol lysate to a mortar, continue to grind to a uniform liquid without obvious tissue mass, and then transfer to a 1.5ml eppendorf tube without RNase; 3...

Embodiment 2

[0084] Example 2. Using RT-qPCR technology to detect the differential expression of lncRNA combination in the serum of patients with esophageal squamous cell carcinoma and healthy people

[0085] 1. Serum RNA extraction The present invention uses a spin column type RNA rapid extraction kit to extract. The specific steps are as follows: 1) Take 0.25ml serum, add 0.75ml lysis buffer RLS, shake the homogenized sample vigorously, and mix it at 15- Incubate at 30°C for 5 minutes to completely decompose the protein bodies; 2) Centrifuge for 10 minutes at 4°C and 12000 rpm, carefully take the supernatant and transfer it to a new RNase free centrifuge tube; 3) Add 0.15ml chloroform and cover Tighten the cap of the sample tube, shake vigorously for 15s and incubate it for 3min at room temperature; 4) Centrifuge at 4℃ and 12000rpm for 10min, the sample will be divided into three layers: the lower layer is the organic phase, the middle layer and the upper layer are the colorless water phase....

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Abstract

The invention discloses an LncRNA composition for detecting prognosis of an early esophageal cancer and a kit comprising the LncRNA composition. By fluorescent quantitative PCR, a sample (which can be tissues/plasma serum and the like) of a patient with an esophageal cancer and difference change of the expression amount of a group of LncRNA in a corresponding para-carcinoma tissue/reference sample are identified, and the risk of esophageal cancerrelapse or transfer is accurately evaluated early. The kit can be used for accurately detecting early esophageal cancer transfer potential, then the transfer or relapse risk of a postoperative esophageal cancer patient can be accurately predicted and evaluated, the patient with high risk is intensively monitored and intervened effectively, relapse or transfer of the esophageal cancer is reduced, and prognosis of the patient is improved further. The poor prognosis related lncRNA composition is adopted, and the shortcomings that sensitivity and specificity are low due to the fact that a kit only uses single lncRNA as a tumor marker, and therefore, rate of fault diagnosis and rate of missed diagnosis on the esophageal cancer are greatly increased are overcome.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an LncRNA combination for detecting the prognosis of early esophageal cancer and a kit containing the combination. Background technique [0002] Esophageal cancer is a common malignant tumor of the digestive tract. There are two main pathological types: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC). In Western countries, adenocarcinoma is the main type, and more than 95% of my country are mainly squamous cell carcinoma, which is the fourth cause of cancer death in my country. Bit. The onset of ESCC is a complex process with multiple factors, multiple genes, and multiple steps. It is highly invasive. The overall survival rate after advanced ESCC treatment is only 15%-20%, while the 5-year survival rate after early ESCC surgery can reach 80%. %the above. The clinical symptoms of early ESCC are not typical, and the diagnosis is confirmed b...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/686C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178C12Q2521/107C12Q2563/107C12Q2545/114
Inventor 徐茜阚云超姚伦广彭毅蕾李丹丹张冰李娜邱礽姚路路赵珂李惠
Owner NANYANG NORMAL UNIV
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