Fermentation medium and production process for producing oritavancin precursor A82846B

A technology of A82846B, fermentation medium, applied in the field of fermentation medium, can solve the problems of low yield and high impurity content, and achieve the effect of increased yield, low impurity content, and increased yield

Active Publication Date: 2018-01-09
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem solved by the present invention is to overcome the shortcomings of the existing fermentation medium for culturing Amycolatopsis orientalis NRRL18099 to prod

Method used

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  • Fermentation medium and production process for producing oritavancin precursor A82846B
  • Fermentation medium and production process for producing oritavancin precursor A82846B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] In this example 1, in the fermentation medium, glucose is used as the carbon source, and its dosage is 5.0%, and the other conditions and production process are the same as those of comparative example 1.

[0049] As determined by HPLC, the yield of A82846B was 1600mg / L. It can be seen that when 5.0% glucose is used as the carbon source, the yield of A82846B is increased by 11.3% compared to Comparative Example 1.

Embodiment 2-3

[0055] In Examples 2 and 3, glucose was used as the carbon source in the fermentation medium, and the dosages were 6.5% and 8.0% respectively, and other conditions and production processes were the same as in Comparative Example 1.

[0056] As determined by HPLC, the yield of A82846B is as follows:

[0057] Numbering

[0058] It can be seen that the best fermentation result is when 8.0% glucose is used as the carbon source, and the yield of A82846B is increased by 19.96% compared with Comparative Example 1.

Embodiment 4

[0068] In Example 4, in the fermentation medium, the carbon source is glucose, and its dosage is 8.0%; the nitrogen source is yeast powder, and its dosage is 0.5%; other conditions and production process are the same as those of Comparative Example 1.

[0069] As determined by HPLC, the yield of A82846B was 1790mg / L. In combination with Comparative Examples 9-11, it can be seen that the best fermentation result is using 0.5% yeast powder as nitrogen source, and the yield of A82846B is increased by 24.48% compared with Comparative Example 1.

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Abstract

The invention discloses a fermentation medium and a production process for producing an oritavancin precursor A82846B. The fermentation medium is used for producing the oritavancin precursor A82846B,and the fermentation medium consists of an organic carbon source, an organic nitrogen source, mineral substances and water, wherein the organic carbon source includes 5-10% of glucose in terms of themass percentage of the fermentation medium, and the organic nitrogen source includes 0.3-1.0% of yeast powder in terms of the mass percentage of the fermentation medium. With the application of the fermentation medium provided by the invention, the yield of the A82846B is greatly improved, and the proportions of two impurities, namely A82846A and A82846C, are obviously reduced. The production process for producing the oritavancin precursor A82846B provided by the invention can effectively improve the yield of the A82846B and reduce the content of the impurities; the production process is applicable to production by virtue of fermenters; and efficient production of the oritavancin precursor A82846B can be achieved.

Description

technical field [0001] The invention relates to a fermentation medium and a production process for producing oritavancin precursor A82846B. Background technique [0002] In August 2014, the U.S. FDA approved the new drug Oritavancin (trade name: Orbactiv) to go on the market. Orbactiv is used to treat acute bacterial skin and skin structure infections and is a second-generation glycopeptide antibiotic developed after vancomycin and teicoplanin. It is a structural analogue of vancomycin, both of which contain a heptapeptide backbone, and its mode of action is similar to that of vancomycin, inhibiting the formation of bacterial cell walls by blocking the transglycosidic and transpeptidic effects of peptidoglycan biosynthesis . Antibacterial activity studies have shown that oritavancin is a broad-spectrum antibacterial drug that can rapidly kill Gram-positive aerobic and anaerobic bacteria, including Staphylococcus, Enterococcus, Streptococcus, Clostridium difficile, Clostri...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12R1/01
Inventor 陈少欣杨松柏王伟艳
Owner SHANGHAI INST OF PHARMA IND
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