Method for extracting anthocyanin from blueberry
A blueberry anthocyanin and extraction method technology, which is applied in the field of blueberry anthocyanin extraction, can solve the problems of low extraction rate of blueberry anthocyanin, achieve good development prospects, simple technology, and easy operation
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Embodiment 1
[0024] (1) Take 60 parts of high-quality rice, wash it with water, add water to soak until the rice has no hard core, put the soaked rice into the rice cooker and cook until the rice is cooked and not sticky, then divide the cooked rice Pack into a container, inoculate Aspergillus oryzae and mix evenly, and ferment for 4 days at 35°C and humidity above 90%; the inoculation amount of Aspergillus oryzae is 2%;
[0025] (2) Put the rice fermented for 4 days into a container, add 10% of its mass phosphate buffer, homogenize for 3 minutes, centrifuge at 4200r / min for 5 minutes, and take the supernatant for later use; the pH of the phosphate buffer is 6.5 ;
[0026] (3) Wash the fresh blueberry fruit, use liquid nitrogen to quickly freeze the fresh blueberry fruit, quickly mash the blueberry, and then add 5% of the mass of the supernatant obtained in step (2), mix evenly, and use a freeze dryer after 10 minutes. Freeze-drying at 80°C to obtain dry blueberry powder; the moisture in ...
Embodiment 2
[0031] (1) Take 80 parts of high-quality rice, wash it with water, add water to soak until the rice has no hard core, put the soaked rice into the rice cooker and cook until the rice is cooked and not sticky, then divide the cooked rice Pack into a container, inoculate and mix with Aspergillus oryzae, and ferment for 6 days at 35°C and humidity above 90%; the inoculation amount of Aspergillus oryzae is 4%;
[0032] (2) Put the rice fermented for 6 days into a container, add 10-20% of its mass in phosphate buffer, homogenize for 3 minutes, centrifuge at 4200r / min for 5 minutes, and take the supernatant for later use; the pH of the phosphate buffer is is 7.5;
[0033] (3) Wash the fresh blueberry fruit, use liquid nitrogen to freeze the fresh blueberry fruit, quickly mash the blueberry, and then add 5-10% of the mass of the supernatant obtained in step (2), mix well, and use a freeze dryer after 30 minutes Freeze-drying at -80°C to obtain dry blueberry powder; the moisture in t...
Embodiment 3
[0038] (1) Take 70 parts of high-quality rice, wash it with water, add water to soak until the rice has no hard core, put the soaked rice into the rice cooker and cook until the rice is cooked and not sticky, then divide the cooked rice Pack it into a container, inoculate Aspergillus oryzae and mix well, and ferment for 4 days at 35°C and humidity above 90%; the inoculation amount of Aspergillus oryzae is 3%;
[0039] (2) Put the rice fermented for 4 days into a container, add 10-20% of its mass in phosphate buffer, homogenize for 3 minutes, centrifuge at 4200r / min for 5 minutes, and take the supernatant for later use; the pH of the phosphate buffer is is 7;
[0040] (3) Wash the fresh blueberries, and use liquid nitrogen to quickly freeze the fresh blueberries, quickly mash the blueberries, and then add 8% of the mass of the supernatant obtained in step (2), mix well, and use a freeze dryer after 20 minutes. Freeze-drying at 80°C to obtain dry blueberry powder; the moisture ...
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