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Method for extracting anthocyanin from blueberry

A blueberry anthocyanin and extraction method technology, which is applied in the field of blueberry anthocyanin extraction, can solve the problems of low extraction rate of blueberry anthocyanin, achieve good development prospects, simple technology, and easy operation

Inactive Publication Date: 2018-01-12
广西南宁桂尔创环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the extraction rate of blueberry anthocyanin is still very low in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Take 60 parts of high-quality rice, wash it with water, add water to soak until the rice has no hard core, put the soaked rice into the rice cooker and cook until the rice is cooked and not sticky, then divide the cooked rice Pack into a container, inoculate Aspergillus oryzae and mix evenly, and ferment for 4 days at 35°C and humidity above 90%; the inoculation amount of Aspergillus oryzae is 2%;

[0025] (2) Put the rice fermented for 4 days into a container, add 10% of its mass phosphate buffer, homogenize for 3 minutes, centrifuge at 4200r / min for 5 minutes, and take the supernatant for later use; the pH of the phosphate buffer is 6.5 ;

[0026] (3) Wash the fresh blueberry fruit, use liquid nitrogen to quickly freeze the fresh blueberry fruit, quickly mash the blueberry, and then add 5% of the mass of the supernatant obtained in step (2), mix evenly, and use a freeze dryer after 10 minutes. Freeze-drying at 80°C to obtain dry blueberry powder; the moisture in ...

Embodiment 2

[0031] (1) Take 80 parts of high-quality rice, wash it with water, add water to soak until the rice has no hard core, put the soaked rice into the rice cooker and cook until the rice is cooked and not sticky, then divide the cooked rice Pack into a container, inoculate and mix with Aspergillus oryzae, and ferment for 6 days at 35°C and humidity above 90%; the inoculation amount of Aspergillus oryzae is 4%;

[0032] (2) Put the rice fermented for 6 days into a container, add 10-20% of its mass in phosphate buffer, homogenize for 3 minutes, centrifuge at 4200r / min for 5 minutes, and take the supernatant for later use; the pH of the phosphate buffer is is 7.5;

[0033] (3) Wash the fresh blueberry fruit, use liquid nitrogen to freeze the fresh blueberry fruit, quickly mash the blueberry, and then add 5-10% of the mass of the supernatant obtained in step (2), mix well, and use a freeze dryer after 30 minutes Freeze-drying at -80°C to obtain dry blueberry powder; the moisture in t...

Embodiment 3

[0038] (1) Take 70 parts of high-quality rice, wash it with water, add water to soak until the rice has no hard core, put the soaked rice into the rice cooker and cook until the rice is cooked and not sticky, then divide the cooked rice Pack it into a container, inoculate Aspergillus oryzae and mix well, and ferment for 4 days at 35°C and humidity above 90%; the inoculation amount of Aspergillus oryzae is 3%;

[0039] (2) Put the rice fermented for 4 days into a container, add 10-20% of its mass in phosphate buffer, homogenize for 3 minutes, centrifuge at 4200r / min for 5 minutes, and take the supernatant for later use; the pH of the phosphate buffer is is 7;

[0040] (3) Wash the fresh blueberries, and use liquid nitrogen to quickly freeze the fresh blueberries, quickly mash the blueberries, and then add 8% of the mass of the supernatant obtained in step (2), mix well, and use a freeze dryer after 20 minutes. Freeze-drying at 80°C to obtain dry blueberry powder; the moisture ...

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Abstract

The invention discloses a method for extracting anthocyanin from blueberry. The method comprises the following steps of using aspergillus oryzae-fermented rice extract as an extracting additive to assist microwave treatment, extracting by ethyl acetate, and purifying by D101 macroporous resin; finally, eluting by ethyl alcohol, and freeze-drying, so as to prepare the anthocyanin powder of the blueberry. The method has the advantages that compared with the traditional technology, the content of anthocyanin in the prepared anthocyanin powder of the blueberry is increased by 10 to 14%; the technical foundation is provided for the production and processing of the anthocyanin.

Description

technical field [0001] The invention belongs to a method for preparing natural biological materials, in particular to a method for extracting blueberry anthocyanins. Background technique [0002] Blueberry fruit is rich in nutrients, which has the functions of preventing brain nerve aging, protecting eyesight, strengthening the heart, anti-cancer, softening blood vessels, enhancing human body immunity, etc., and has high nutritional components. The anthocyanins contained in blueberries are several times or even dozens of times that of other fruits such as apples and grapes. The total amino acid content is 0.254%, which is higher than that of hawthorn. Blueberry anthocyanins have strong antioxidant properties, have good curative effects on ophthalmology and cardiovascular and cerebrovascular diseases, and can play a variety of effects such as beauty and health care. However, the extraction rate of blueberry anthocyanins is still very low in the prior art. [0003] For examp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
Inventor 戚海冰
Owner 广西南宁桂尔创环保科技有限公司
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