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Oil and gas microbial recovery solution, oil and gas microbial sample recovery method and application

A microbial and oil-gas technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve problems that affect the accuracy and stability of microbial exploration, cannot meet the requirements of actual production, and affect the accuracy of microbial exploration, etc., to achieve good results Resuscitation effect, improved accuracy and reliability, effect of simple steps

Active Publication Date: 2020-05-22
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is, in the field, an oven is used to remove most of the moisture in the sample, so that the microorganisms in it are in a dormant state. This method will also cause the death of some moisture-sensitive microorganisms, thereby affecting the accuracy of microbial exploration.
[0006] To sum up, the current problems and shortcomings in the processing methods of oil and gas microbial exploration samples are: no matter whether the rapid freezing method or the rapid air drying method is adopted, a large part of sensitive microorganisms will die, and at the same time, there are no samples before microbial culture counting. Complementary recovery methods will inevitably affect the accuracy and stability of microbial exploration in the end
It can be seen that the existing sample pretreatment technology can not meet the requirements of actual production

Method used

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  • Oil and gas microbial recovery solution, oil and gas microbial sample recovery method and application
  • Oil and gas microbial recovery solution, oil and gas microbial sample recovery method and application
  • Oil and gas microbial recovery solution, oil and gas microbial sample recovery method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Stir and sieve the sample (sand sample or clay sample) containing oil and gas microorganisms, the sieve is 20 mesh, and weigh 15 g of the sample. Then gradually lower the temperature of the sample containing oil and gas microorganisms, control the cooling rate to 1°C / min, cool down to -35°C, and keep it for a period of time, so that the total time from the start of cooling plus the holding time is 2h, and then put the sample into - Keep at 70°C for 2h. Then, freeze and vacuum-dry the above sample at -50° C. with a vacuum degree of 0.15 MPa, so that the water content of the sample is 1 wt%. The samples were then stored at 4°C in the dark for 3 months. The preserved sample was dissolved in resuscitation solution 1 (see Table 1), shaken on a shaker at 70 rpm for 10 h at room temperature to obtain a sample containing resuscitated oil and gas microorganisms, and the oil and gas microorganisms therein were cultured and detected, and the results are shown in Table 2.

Embodiment 2

[0048] Same as Example 1, except that the resuscitation solution is resuscitation solution 2 (see Table 1), and has not been preserved. Before dissolving the pretreated oil and gas microorganism sample in resuscitation solution 2, put it into a water bath at 38±2°C for rapid thawing, and the thawing time is 1-2h. After recovery treatment and shaking treatment, samples containing recovered oil and gas microorganisms were obtained, and the oil and gas microorganisms were cultured and detected, and the results are shown in Table 2.

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Abstract

The invention relates to a resuscitation solution for microbes in oil gas. In the resuscitation solution, a mol ratio of elemental nitrogen to elemental phosphorus is (0.97-1.63): 1; a mol concentration of elemental potassium is 0.021 to 0.033 mol / L; a mol concentration of elemental sulfur is 0.0008 to 0.012 mol / L; and a mol concentration of elemental sodium is 0.017 to 0.048 mol / L. The inventionalso relates to a resuscitation method for oil gas microbes. Through pretreatment and resuscitation of an oil gas microbe sample, oil gas microbes used for culture and detection can be obtained. The resuscitation solution provided by the invention is simple in composition and can maximally reproduce field in-situ microfloral quantity and structure. The oil gas microbes acquired through the resuscitation method are high in survival rate, and the method has good resuscitation effect on typical oil gas microbes. The resuscitation solution for oil gas microbes and the resuscitation method for oilgas microbes in the invention are of great significance to microbial prospecting of oil and gas.

Description

technical field [0001] The invention belongs to the technical field of recovery of oil and gas microorganisms, and in particular relates to a solution for recovery of oil and gas microorganisms, a method for recovery of oil and gas microorganism samples and applications thereof. Background technique [0002] Microbial exploration is currently a hot spot in the development of oil and gas resources. Its technical system is gradually established based on the vertical migration theory of volatile or semi-volatile hydrocarbons in underground oil and gas reservoirs. With the continuous supply of a certain concentration of hydrocarbons in the surface soil, the microorganisms in the oxidized layer that use hydrocarbons as the sole carbon source can grow and multiply rapidly. By measuring the abundance difference of these oil and gas indicator microorganisms, it can be used to indirectly characterize the light hydrocarbons in the soil. Concentration level, so as to predict the oil a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12Q1/64C12Q1/06
Inventor 许科伟汤玉平杨帆顾磊高俊阳
Owner CHINA PETROLEUM & CHEM CORP
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