Application of glioma prognostic marker Circ9:135881633|135883078

A glioma and prognosis technology, applied in the application of reagents in the preparation of glioma prognostic preparations, and the application field of circRNA markers, can solve the problems that the survival rate of glioma patients has not been significantly improved, and the survival rate is not optimistic.

Inactive Publication Date: 2018-01-16
XIANGYA HOSPITAL CENT SOUTH UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of glioma prognostic marker Circ9:135881633|135883078
  • Application of glioma prognostic marker Circ9:135881633|135883078
  • Application of glioma prognostic marker Circ9:135881633|135883078

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of reagents for detecting expression of circular RNA Circ9:135881633|135883078 Kit for preparing prognosis of glioma patients (50 reactions)

[0018] 1. RNA stabilization solution 50ml

[0019] 2. Isopropanol 100ml

[0020] 3. Chloroform 100ml

[0021] 4. Trizol 50ml

[0022] 5. Enzyme-free water 10ml

[0023] 6. 1μM random reverse transcription primer 50μl

[0024] 7. 5× reverse transcription buffer 200ml

[0025] 8. 100μl of 10mM base triphosphate deoxyribonucleotides

[0026] 9. 40U / μl RNase inhibitor 500μl

[0027] 10. 200U / μl MMLV reverse transcriptase 50μl

[0028] 11.Premix Ex Taq 50μl

[0029] 12. 10μM Circ9:135881633|135883078 real-time fluorescence quantitative PCR specific primer 30μl

[0030] Circular RNA Circ9:135881633|135883078 Forward primer:

[0031] 5'-AATGGTGGATGCCCTGAT-3',

[0032] Circular RNA Circ9:135881633|135883078 reverse primer:

[0033] 5'-TGTGCTCCTGCTCATACTGG-3';

[0034] 13. 10μM GAPDH specific primer 30μl...

Embodiment 2

[0037] Example 2 Detection of expression level of circular RNA Circ9:135881633|135883078 in brain tissue samples

[0038] 1. Collect the glioma tissue to be tested, put it into a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0039] 2. Extraction of RNA in the tissue: take 0.1g of the above-mentioned tumor and normal brain tissue, rinse with an appropriate amount of normal saline, and put it into a mortar (the mortar must be wrapped with tinfoil in advance and baked in an oven at 180 degrees for 6-8 hours). hours), add an appropriate amount of liquid nitrogen to grind the tissue to powder, add 0.6ml Trizol (MRC company) and continue grinding for a few minutes, add 0.4ml Trizol again, transfer the mixture in the mortar to a 1.5ml enzyme-free Tube tube, and put it on ice Lyse for 15 minutes. After the lysis, transfer to a 1.5ml enzyme-free tube, centrifuge at 12000rpm at 4°C for 10min, and transfer the supernata...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biology and discloses application of a glioma prognostic marker Circ9:135881633|135883078, in particular to application of a reagent for detecting the expression quantity of cyclic RNA (Ribonucleic Acid) Circ9:135881633|135883078 from a brain tissue in preparing a prognostic preparation of a glioma patient. The study proves that patients with higherexpression quantity of the cyclic RNA Circ9:135881633|135883078 in the glioma have a higher postoperative survival rate. Prognostic judgement of the glioma patients is made by detecting the expressionlevel of the cyclic RNA Circ9:135881633|135883078 in glioma tissues of the glioma patients.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of a brain tissue-derived circRNA marker for glioma prognosis, that is, the application of a reagent for detecting the marker in the preparation of a glioma prognosis preparation. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 武明花冯紫阳李沛瑶刘庆范礼刘强王蓉
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap