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Extracting method of stilbene glycoside in fleeceflower roots

A technology of stilbene glycosides and extraction methods, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of poor purification effect of macroporous resins, and achieve the effect of low cost and simple process

Inactive Publication Date: 2018-01-19
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prior art generally first extracts with organic solvents, and then uses macroporous resins to purify stilbene glycosides, such as AB-8, S-8, X-5, NKA-9, but the purification effect of macroporous resins is relatively poor. The purity is only 70-80%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Pulverize Polygonum multiflorum to 80 mesh, add ethanol solution with a volume concentration of 60% according to the ratio of material to liquid ratio of 1g:10ml, and extract under reflux at 30°C for 1 hour to obtain an ethanol extract;

[0028] 2) recovering the ethanol extract under reduced pressure to an extract with a relative density of 1.0 at 40° C., adding water 10 times its volume to dissolve the extract, filtering, and taking the filtrate for later use;

[0029] 3) Put the S-8+ macroporous resin on the filtrate, the sample loading flow rate is 1BV / h, and the distilled water is eluted first, and the elution flow rate of the distilled water is 2BV / h, and then it is eluted with a volume concentration of 60% ethanol solution, and the ethanol wash The flow rate is 1BV / h, and the ethanol eluate is collected;

[0030] Described S-8+ resin is polystyrene type polar adsorption resin, and quality index is as follows:

[0031] Appearance: milky white opaque spherical ...

Embodiment 2

[0044] 1) Pulverize Polygonum multiflorum to 80 mesh, add ethanol solution with a volume concentration of 80% according to the ratio of material to liquid ratio of 1g:20ml, and extract under reflux at 45°C for 3 hours to obtain an ethanol extract;

[0045] 2) recovering the ethanol extract under reduced pressure to an extract with a relative density of 1.5 at 40°C, adding the extract to 15 times its volume in water to dissolve, filtering, and taking the filtrate for later use;

[0046] 3) Put the filtrate on the S-8+ macroporous resin, the flow rate of sample loading is 3BV / h, first elute with distilled water, the elution flow rate of distilled water is 5BV / h, then elute with a volume concentration of 60% ethanol solution, wash with ethanol The flow rate is 2BV / h, and the ethanol eluate is collected;

[0047] Described S-8+ resin is polystyrene type polar adsorption resin, and quality index is as follows:

[0048] Appearance: milky white opaque spherical particles;

[0049] ...

Embodiment 3

[0061] 1) Pulverize Polygonum multiflorum to 80 mesh, add ethanol solution with a volume concentration of 70% according to the ratio of material to liquid ratio of 1g:15ml, and extract under reflux at 40°C for 2 hours to obtain an ethanol extract;

[0062] 2) recovering the ethanol extract under reduced pressure to an extract with a relative density of 1.2 at 40°C, adding the extract to 12 times its volume in water to dissolve, filtering, and taking the filtrate for later use;

[0063] 3) Put the S-8+ macroporous resin on the filtrate, the sample loading flow rate is 2BV / h, first elute with distilled water, the elution flow rate of distilled water is 4BV / h, then use a volume concentration of 60% ethanol solution to elute, ethanol wash The flow rate is 1.5BV / h, and the ethanol eluate is collected;

[0064] Described S-8+ resin is polystyrene type polar adsorption resin, and quality index is as follows:

[0065] Appearance: milky white opaque spherical particles;

[0066]Moist...

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Abstract

The invention discloses an extracting method of stilbene glycoside in fleeceflower roots. The extraction method comprises the following steps: (1) smashing fleeceflower roots, and adding ethanol for reflux extraction so as to obtain an ethanol extracting solution; (2) decompressing the ethanol extracting solution to recover an extract with the relative density being 1.0-1.5 at 40 DEG C, adding water into the extract to dissolve, filtering and taking filtrate for later use; (3) adding S-8+ macroporous resin for the filtrate, carrying out elution with distilled water firstly, then carrying out elution with 60% ethanol, and collecting ethanol eluant; and (4) decompressing the ethanol eluant to recover a solvent, and carrying out freeze drying, thereby obtaining stilbene glycoside. According to the extracting method, the process is simple, the cost is low, and the purity of the prepared stilbene glycoside reaches up to 99% or above.

Description

technical field [0001] The invention belongs to the technical field of extraction of bioactive components, and in particular relates to a method for extracting stilbene glycosides from Polygonum multiflorum. Background technique [0002] Polygonum multiflorum is a clinical traditional Chinese medicine. It is the dry root tuber of Polygonaceae plants. my country has rich plant resources and is distributed in the northeast, south-central, east and southwest provinces. After processing, Polygonum multiflorum has the functions of nourishing liver and kidney, benefiting essence and blood, black beard and hair, strengthening muscles and bones, transforming turbidity and lowering fat. Hyperlipidemia, etc. Modern studies have found that Polygonum multiflorum contains various components such as anthraquinones, stilbene glycosides and flavonoids. The main pharmacological activities include promoting hematopoietic function, enhancing immune function, lowering blood lipids and anti-ath...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H15/18
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司