Automatic and integrated system for extracting, amplifying and detecting nucleic acids

Abstract

The invention discloses an automatic and integrated system for extracting, amplifying and detecting nucleic acids. The system is matched with a disposable and enclosed double face disc type reaction box of a prepackaged reagent, in order to realize automatic nucleic acid detection with 'a sample enters and a result outputs'. The system contains a driving control carrying platform for loading, rotating and exerting the double-face disc type reaction box from the bottom, and a top control component for exerting the same reaction box from the top. The driving control carrying platform and the topcontrol component are provided with an execution device for vertically acted on the double-face disc type reaction box, the execution device provides experimental operations, such as fixation and rotary positioning of the reaction box, unidirectional or reciprocating rotation of the reaction box, high speed centrifugation for driving fluid to flow, opening or enclosing of a sample injection port,a valve and a channel and other switches or seal membranes on the reaction box, heating of the prepackaged reagent and adsorption of magnetic beads, multizone temperature control and multipath fluorescence detection, and the like; automatic and integrated extraction, amplification and detection of nucleic acid in the enclosed reaction box are realized.

Description

technical field [0001] The invention belongs to the field of biomedical gene analysis instruments, in particular to a fully automatic integrated nucleic acid extraction, amplification and detection system. Background technique [0002] Gene detection analysis is the basic experimental method of modern biomedicine. Molecular diagnosis is a technology that uses molecular biological methods to detect changes in the structure or expression level of exogenous or self-genetic material in patients to make a diagnosis. It is one of the important frontier fields in the development of contemporary medicine. The basis of molecular diagnosis is the analysis of target nucleic acid, and its conventional techniques include: fluorescent PCR, fluorescence in situ hybridization (FISH), DNA sequencing, Southern blotting, single nucleotide polymorphism (SNP), ligase chain reaction (LCR) ) and gene chips, etc. No matter what kind of operation and detection is performed on these target nucleic a...

AI Technical Summary

Problems solved by technology

This makes most of the current molecular diagnostics need to be completed in the laboratory by larger instruments, which greatly limits the application range of molecular diagnostics

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