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Method of quickly identifying robinia pseudoacacia polyploid on basis of fluorescent SSR (simple sequence repeat) capillary electrophoresis technology

A technology of capillary electrophoresis and polyploidy, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, etc., can solve the problems of rapid identification of polyploidy of Robinia pseudoacacia, and achieve high-efficiency identification, convenient operation, and good amplification efficiency Effect

Active Publication Date: 2018-01-19
SHANDONG FOREST SCI RES INST +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is no method for rapid identification of Robinia pseudoacacia polyploids using fluorescent SSR labeling technology at home and abroad.

Method used

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  • Method of quickly identifying robinia pseudoacacia polyploid on basis of fluorescent SSR (simple sequence repeat) capillary electrophoresis technology
  • Method of quickly identifying robinia pseudoacacia polyploid on basis of fluorescent SSR (simple sequence repeat) capillary electrophoresis technology
  • Method of quickly identifying robinia pseudoacacia polyploid on basis of fluorescent SSR (simple sequence repeat) capillary electrophoresis technology

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Embodiment Construction

[0027] In order to better understand the technical solutions of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0028] 1. Experimental materials:

[0029] In this experiment, five tetraploid Robinia pseudoacacia clones introduced from Korea were used as test materials. They are K1 (Tetraploid R. pseudoacacia K1), K2 (Tetraploid R. pseudoacacia K2), K3 (Tetraploid R. pseudoacacia K3), K4 (Tetraploid R. pseudoacacia K4), and K5 (Tetraploid R. pseudoacacia K5).

[0030] The materials were collected from Daqingshan Forest Farm, Fei County, Shandong Province.

[0031] In May 2016, the young leaves were collected, dried on silica gel, and set aside.

[0032] 2. Genomic DNA extraction:

[0033] Take the young leaves of Robinia pseudoacacia clones, and extract them with Genomic DNA Extraction Kit from Plant Tissue (Magnetic Beads Method) (Catalog No. PTED-6030) of Inrich Biochemic...

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Abstract

The invention discloses a method of quickly identifying a robinia pseudoacacia polyploid on the basis of a fluorescent SSR (simple sequence repeat) capillary electrophoresis technology. The method comprises the steps of taking robinia pseudoacacia genome DNA (deoxyribonucleic acid) as a template, performing SSR-PCR (polymerase chain reaction) amplification by using a primer shown as SEQ ID NOS. (sequence identifier numbers) 1-2 to form a PCR amplification product, namely capillary electrophoresis, constructing a robinia pseudoacacia SSR fingerprint spectrum according to an electrophoresis result, and determining a plant as the polyploid if the number of allelic genes is greater than or equal to 3 according to the constructed robinia pseudoacacia SSR fingerprint spectrum. The method can identify whether the robinia pseudoacacia plant is the polyploid simply, conveniently and quickly; a novel practical and feasible method easy and simple to operate is provided for ploidy analysis and identification of robinia pseudoacacia; a good foundation is established for parent selection of robinia pseudoacacia hybridization, genetic resource storage and evaluation, new variety protection and the like.

Description

technical field [0001] The invention belongs to the technical field of plant polyploid identification, and in particular relates to a method for quickly identifying whether a pseudoacacia plant is polyploid by using a fluorescent SSR marking technology. Background technique [0002] Robinia pseudoacacia L. is a broad-leaved tree of the genus Robinia in the leguminous family (Leguminosae). It is resistant to drought, barrenness, mild salinity, pollution, and natural renewal. Pioneer tree species with high ornamental, nectar and feeding value. Robinia locust wood is tough, impact-resistant, decay-resistant, and resistant to water and humidity. It is an important material for construction, ore pillars, vehicles, and shipbuilding. Robinia locust wood has a high calorific value and a slow burning speed. It is a high-quality fuel wood and an excellent biomass energy tree species. Black locust is native to North America, and the worldwide introduction and domestication began in 16...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6806
Inventor 毛秀红荀守华张元帅孙百友乔玉玲董玉峰韩丛聪
Owner SHANDONG FOREST SCI RES INST