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Method using mulberry silk to prepare viscose silk fibroin hydrogel

A technology of silk fibroin and regenerated silk fibroin, applied in the field of preparation of viscous silk fibroin hydrogel, can solve the problems of hyaluronic acid lack of mechanical strength, short retention time, high price, etc., and achieve considerable social and economic benefits , Improve the effect of mechanical strength

Active Publication Date: 2018-01-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of lack of mechanical strength, short retention time in the body, and high price of the currently widely used hyaluronic acid, and open up a new application field of silk fibroin materials, a kind of hyaluronic acid that can replace hyaluronic acid is prepared. Soft Tissue Fillers

Method used

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  • Method using mulberry silk to prepare viscose silk fibroin hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Put 50g of mulberry silk into 2.5L boiling water containing 15g of melted soap for degumming treatment for 30min, remove the fibrous silk and wring it out, fully wash the soap with deionized water, repeat the above operation twice, Dry in an oven at 60°C for 15 hours to obtain desericinized silk;

[0018] 2) Dissolve the desericin silk obtained in step 1) in 50ml of LiBr solution with a concentration of 9.3M, put it in an oven at 60°C for 5 hours, and put the resulting solution in a dialysis bag and dialyze it with deionized water for 3 days , change the deionized water every 12h, and then use 9.0wt% polyethylene glycol (PEG) solution for reverse dialysis for 12h, then centrifuge the solution at 8000rpm for 30min, remove undissolved residue, and put it in 4°C Store in a refrigerator to obtain a high-concentration regenerated silk fibroin solution with a mass fraction of 6.0 wt %;

[0019] 3) Mix the high-concentration regenerated silk fibroin solution obtained in st...

Embodiment 2

[0021] 1) Put 50g of mulberry silk into 2.5L boiling water containing 15g of melted soap for degumming treatment for 35min, remove the fibrous silk and wring it out, fully wash the soap with deionized water, repeat the above operation 3 times, Dry in an oven at 55°C for 12 hours to obtain desericinized silk;

[0022] 2) Dissolve the desericin silk obtained in step 1) in 50ml of LiBr solution with a concentration of 9.3M, put it in an oven at 65°C for 4 hours, and put the resulting solution in a dialysis bag and dialyze it with deionized water for 3 days , change the deionized water every 12h, and then use 8.0wt% polyethylene glycol (PEG) solution for reverse dialysis for 18h, and then centrifuge the solution at 8000rpm for 40min to remove undissolved residue, and put it in 4°C Store in a refrigerator to obtain a high-concentration regenerated silk fibroin solution with a mass fraction of 7.0 wt %;

[0023] 3) Mix the high-concentration regenerated silk fibroin solution obtain...

Embodiment 3

[0025] 1) Put 50g of mulberry silk into 2.5L boiling water containing 15g of melted soap for degumming treatment for 40min, remove the fibrous silk and wring it out, fully wash the soap with deionized water, repeat the above operation twice, Dry in an oven at 65°C for 18 hours to obtain desericinized silk;

[0026] 2) Dissolve the desericin silk obtained in step 1) in 50ml of LiBr solution with a concentration of 9.3M, put it in an oven at 55°C for 3 hours, and put the resulting solution in a dialysis bag and dialyze it with deionized water for 3 days , change the deionized water every 12h, and then use 10.0wt% polyethylene glycol (PEG) solution for reverse dialysis for 15h, and then centrifuge the solution at 8000rpm for 35min to remove undissolved residue, and put it in 4°C Store in a refrigerator to obtain a high-concentration regenerated silk fibroin solution with a mass fraction of 8.0 wt %;

[0027] 3) Mix the high-concentration regenerated silk fibroin solution obtaine...

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Abstract

The invention discloses a method using mulberry silk to prepare viscose silk fibroin hydrogel. The method mainly includes: selecting mulberry silk fibroin which is good in biocompatibility and wide insource to serve as the base material, using polyethylene glycol as the auxiliary material, and adjusting the pH of a mixed solution to prepare the novel gel with certain viscosity. The novel gel is used for soft tissue injection filling. The method has the advantages that the method is simple to operate, does not need precious equipment and is high in biosafety; the prepared fibroin gel can be easily retained in a body and is less prone to loss, injection filling times can be reduced, resource waste and patient economic burden are reduced, a new application field of fibroin materials is developed, and considerable social and economic benefits are achieved.

Description

technical field [0001] The invention relates to a method for preparing viscous silk fibroin hydrogel, in particular to a method for preparing viscous silk fibroin hydrogel by using mulberry silk, and belongs to the field of soft tissue engineering. Background technique [0002] In recent years, injectable gels for microplastic surgery have developed rapidly. Among them, sodium hyaluronate is widely used because of its good biocompatibility and immunogenicity. However, sodium hyaluronate gels improved by cross-linking Glue can only last for 3-6 months in the human body. Repeated injections are required to maintain the effect of micro-plastic surgery, which increases safety risks, patient pain and economic burden. Clinical needs are biodegradable and viscoelastic. Effective shaping soft tissue filler. [0003] Mulberry silk is a natural polymer material with excellent biocompatibility. Silk fibroin obtained from degumming of mulberry silk is a fibrous protein. Silk fibroin ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00C08L71/02A61L27/26A61L27/52
Inventor 姚菊明袁梅华蔡玉荣徐江晖
Owner ZHEJIANG SCI-TECH UNIV
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