Method using mulberry silk to prepare viscose silk fibroin hydrogel

A technology of silk fibroin and regenerated silk fibroin, applied in the field of preparation of viscous silk fibroin hydrogel, can solve the problems of hyaluronic acid lack of mechanical strength, short retention time, high price, etc., and achieve considerable social and economic benefits , Improve the effect of mechanical strength

Active Publication Date: 2018-01-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of lack of mechanical strength, short retention time in the body, and high price of the currently widely used hyaluronic

Method used

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  • Method using mulberry silk to prepare viscose silk fibroin hydrogel

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Experimental program
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Effect test

Example Embodiment

[0016] Example 1:

[0017] 1) Put 50g of mulberry silk into 2.5L boiling water containing 15g of melted soap for degumming for 30 minutes. Take out the fibrous silk and wring dry. Wash the soap thoroughly with deionized water. Repeat the above operation twice. Dry it in an oven at 60°C for 15 hours to obtain deserified silk;

[0018] 2) Dissolve the desiccated silk obtained in step 1) in 50ml of LiBr solution with a concentration of 9.3M, and dissolve it in an oven at 60°C for 5 hours. Place the resulting solution in a dialysis bag and dialyze with deionized water for 3 days , Replace the deionized water every 12h, then use a 9.0wt% polyethylene glycol (PEG) solution for reverse dialysis to stand for 12h, and then centrifuge the solution at 8000rpm for 30min to remove the undissolved residue and put it at 4℃ Store in a refrigerator to obtain a high-concentration regenerated silk fibroin solution with a mass fraction of 6.0 wt%;

[0019] 3) Mix the high-concentration regenerated sil...

Example Embodiment

[0020] Example 2:

[0021] 1) Put 50g of mulberry silk into 2.5L boiling water containing 15g of melted soap for degumming for 35 minutes. Take out the fibrous silk and wring dry. Wash the soap thoroughly with deionized water. Repeat the above operation 3 times. Dry it in an oven at 55°C for 12 hours to obtain deserified silk;

[0022] 2) Dissolve the desiccated silk obtained in step 1) in 50ml of LiBr solution with a concentration of 9.3M, put it in an oven at 65°C, and dissolve it for 4 hours. Place the resulting solution in a dialysis bag and dialyzed with deionized water for 3 days , Replace the deionized water every 12h, and then use 8.0wt% polyethylene glycol (PEG) solution for reverse dialysis to stand for 18h, and then centrifuge the solution at 8000rpm for 40min, remove the undissolved residue, and put it at 4℃ Store in the refrigerator to obtain a high-concentration regenerated silk fibroin solution with a mass fraction of 7.0 wt%;

[0023] 3) Mix the high-concentration r...

Example Embodiment

[0024] Example 3:

[0025] 1) Put 50g of mulberry silk into 2.5L boiling water containing 15g of melted soap for degumming for 40 minutes. Take out the fibrous silk and wring dry. Wash the soap with deionized water. Repeat the above operation twice. Dry it in an oven at 65°C for 18 hours to obtain desiccated silk;

[0026] 2) Dissolve the desiccated silk obtained in step 1) in 50ml of LiBr solution with a concentration of 9.3M, put it in an oven at 55℃ and dissolve it for 3 hours, place the resulting solution in a dialysis bag and dialyzed with deionized water for 3 days , Replace the deionized water every 12h, and then use a 10.0wt% polyethylene glycol (PEG) solution for reverse dialysis to stand for 15h, and then centrifuge the solution at 8000rpm for 35min, remove the undissolved residue, and put it at 4℃ Store in the refrigerator to obtain a high-concentration regenerated silk fibroin solution with a mass fraction of 8.0 wt%;

[0027] 3) Mix the high-concentration regenerated s...

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Abstract

The invention discloses a method using mulberry silk to prepare viscose silk fibroin hydrogel. The method mainly includes: selecting mulberry silk fibroin which is good in biocompatibility and wide insource to serve as the base material, using polyethylene glycol as the auxiliary material, and adjusting the pH of a mixed solution to prepare the novel gel with certain viscosity. The novel gel is used for soft tissue injection filling. The method has the advantages that the method is simple to operate, does not need precious equipment and is high in biosafety; the prepared fibroin gel can be easily retained in a body and is less prone to loss, injection filling times can be reduced, resource waste and patient economic burden are reduced, a new application field of fibroin materials is developed, and considerable social and economic benefits are achieved.

Description

technical field [0001] The invention relates to a method for preparing viscous silk fibroin hydrogel, in particular to a method for preparing viscous silk fibroin hydrogel by using mulberry silk, and belongs to the field of soft tissue engineering. Background technique [0002] In recent years, injectable gels for microplastic surgery have developed rapidly. Among them, sodium hyaluronate is widely used because of its good biocompatibility and immunogenicity. However, sodium hyaluronate gels improved by cross-linking Glue can only last for 3-6 months in the human body. Repeated injections are required to maintain the effect of micro-plastic surgery, which increases safety risks, patient pain and economic burden. Clinical needs are biodegradable and viscoelastic. Effective shaping soft tissue filler. [0003] Mulberry silk is a natural polymer material with excellent biocompatibility. Silk fibroin obtained from degumming of mulberry silk is a fibrous protein. Silk fibroin ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00C08L71/02A61L27/26A61L27/52
Inventor 姚菊明袁梅华蔡玉荣徐江晖
Owner ZHEJIANG SCI-TECH UNIV
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