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Method for identifying gibberellic disease resistance of aegilops tauschii

The invention relates to a technology for identification of resistance of Goat grass and resistance, which is applied in the field of identification of resistance to head blight, and can solve problems affecting the genetic and breeding work of resistance to head blight in wheat, and no germplasm resources for immunity to head blight and identification of resistance have been found. Difficulties and other problems, to achieve the effect of promoting the inheritance of wheat resistance to Fusarium head blight, intuitive and reliable phenotype, and short identification cycle

Inactive Publication Date: 2018-01-23
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] Wheat head blight resistance may be controlled by multiple genes, the disease resistance mechanism is complex, its pathogenicity is easily affected by environmental conditions, heritability is low, resistance identification is difficult, and resistance resources are scarce. So far, no species immune to head blight has been found. quality resources, these have influenced the genetic and breeding work of wheat head blight resistance to a certain extent

Method used

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  • Method for identifying gibberellic disease resistance of aegilops tauschii
  • Method for identifying gibberellic disease resistance of aegilops tauschii

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Embodiment 1

[0022] The ear type of A. roughis Jing Y188 is moniliform, and it is susceptible to scab; the panicle of A. roughis KU-2074 is moniliform, and it is resistant to scab. Taking these two varieties as examples, using the inventive method to identify their scab resistance, the main steps are as follows:

[0023] 1. the cultivation of Gibberella conidia: select typical strong pathogenicity Fusarium graminearum (bacteria number: SDAU-F.g-04-4) to be resistance identification bacterial classification, at first this bacterial strain is in potato dextrose (PDA ) on a solid medium for activation culture, cultured at a constant temperature of 25°C, after the mycelium is covered with the medium, use a scalpel to cut an appropriate amount of the above medium with mycelium, put it into 40-50mL sterile mung bean soup liquid medium, Shake culture at 25° C. and 200 rpm for 3 days, and then detect spore production by microscope. Filter the bacterial liquid containing enough spores through ster...

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Abstract

The invention relates to a method for identifying gibberellic disease resistance and particularly relates to the method for identifying gibberellic disease resistance of aegilops tauschii. The invention provides a method for high-throughput identification of the gibberellic disease resistance of aegilops tauschii by utilizing the aegilops tauschii in vitro fringe under controllable environmental condition within a short period of time. The method has the major advantage that the evaluation standard for the gibberellic disease resistance of aegilops tauschii is discovered and the gibberellic disease resistance of aegilops tauschii is evaluated according to the extension of the inoculated fringe. The standard is utilized to simply and quickly identify the gibberellic disease resistance of aegilops tauschii with 5-6 days. According to the method, the phenotype identification is direct and reliable, the repeatability is high, the resistance identification for a large batch of materials canbe completed within a short period of time and the method has significance in researching the gibberellic disease resistance of aegilops tauschii all over the world, finding a new gibberellic diseaseresistant resource and widening the gibberellic disease hereditary basis of the wheat.

Description

technical field [0001] The invention relates to a method for identifying resistance to scab disease, in particular to a method for identifying resistance to scab disease of A. tachyphylla. Background technique [0002] Goatgrass was the donor of the wheat D genome. wheat( Triticum aestivum L. ) as one of the world's three major food crops, its yield is second only to corn and rice. However, various biotic and abiotic stresses seriously threaten the yield and quality of wheat during the whole growth and development of wheat. Among them, the abiotic stress factors mainly include salinity, drought, freezing damage, and hot and dry wind, etc.; the biotic stress factors mainly include wheat three rusts (including stripe rust, leaf rust and stem rust), wheat scab and wheat powdery mildew, etc. These three major diseases are the three most serious fungal diseases that threaten the growth of wheat. Among them, fungal pathogens can not only affect the yield of wheat, but also sec...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12Q1/04C12R1/77
Inventor 马信孔令让王宏伟倪同心
Owner SHANDONG AGRICULTURAL UNIVERSITY
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