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Preparation method of biopesticide preparation for control of red imported fire ants

A technology of biological pesticides and red fire ants, applied in the fields of botanical equipment and methods, biocides, animal repellants, etc., can solve the problems of slow speed, affecting the insecticidal activity of Beauveria bassiana, low spore germination rate, etc. And convenient transportation, increase insecticidal effect, improve the effect of germination environment

Active Publication Date: 2018-01-26
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when Beauveria bassiana is used in the field, due to the limitation of temperature, humidity, ultraviolet light and other environmental conditions, the germination rate of spores is low, which affects the insecticidal activity of Beauveria bassiana. Therefore, the use of pathogenic microorganisms Beauveria bassiana alone The rate of control of RIFA by B. anisopliae is relatively slow

Method used

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  • Preparation method of biopesticide preparation for control of red imported fire ants
  • Preparation method of biopesticide preparation for control of red imported fire ants
  • Preparation method of biopesticide preparation for control of red imported fire ants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Indoor virulence determination experiment of Beauveria bassiana and sodium chloride compounded red imported fire ants worker termites

[0026] During indoor toxicity determination, weigh 5 g of Beauveria bassiana powder and dissolve it in 1 L of water, and mix evenly to obtain Beauveria bassiana solution. The used Beauveria bassiana powder contains 20 billion spores of Beauveria bassiana / g. Measure 9 parts of Beauveria bassiana solutions of 100ml each from 1L of Beauveria bassiana solution, and then add NaCl to them respectively, so that the concentrations of NaCl in the 9 parts of Beauveria bassiana solutions are: 0mmol / L, 25mmol / L, 75mmol / L, 100mmol / L, 125mmol / L, 150mmol / L, 200mmol / L, 250mmol / L, 300mmol / L, sequentially obtain Beauveria bassiana · sodium chloride water dispersion solution, wherein the spore concentration of Beauveria bassiana is 1× 10 8 pcs / mL -1 .

[0027]The toxicity of Beauveria bassiana solution, Beauveria bassiana·NaCl aqueous disp...

Embodiment 2

[0030] Embodiment 2: Beauveria bassiana · sodium chloride aqueous dispersant is to the indoor toxicity determination experiment of red fire ants worker termite

[0031] Such as image 3 with Figure 4 As shown, the RIFA control drug preparation mixer of this embodiment includes a liquid container 8, a solid container 4, a rubber gasket 3 and a cover 2, and the solid container 4 is used for opening the upper end and the lower end. A container 5 sealed with aluminum foil composite film, a snap ring 9 is provided on the opening edge of the solid container, and a rubber gasket 6 is placed on the bottle mouth 7 of the liquid container, and the solid container is inserted through the rubber gasket 6 In the bottle mouth 7 of the liquid container, the snap ring 9 is pressed on the rubber gasket 6, the rubber gasket 3 is pressed on the snap ring 9, and the cover 2 presses the rubber gasket 3, the snap ring 9, the rubber The gasket 6 is caught and fixed on the bottle mouth 7 of the li...

Embodiment 3

[0041] During the indoor toxicity test, weigh 1 g of Beauveria bassiana powder and 7.5 g of sodium chloride, dissolve them in 1 L of water, and mix them uniformly to obtain a Beauveria bassiana solution. The used Beauveria bassiana powder contains 20 billion spores of Beauveria bassiana / g.

[0042] The indoor toxicity test was carried out according to the method of Example 1, and the results showed that the Beauveria bassiana solution of the present example had a better killing effect on red fire ants.

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Abstract

The invention discloses a preparation method of a biopesticide preparation for control of red imported fire ants. The method includes: a. preparing a pathogenic microorganism-containing medicament forcontrol of red imported fire ants, wherein the pathogenic microorganism-containing medicament for control of red imported fire ants contains beauveria bassiana and sodium chloride and the ratio of the beauveria bassiana spore number to the sodium chloride gram is (1-8)*20billion:4.0-7.5g; b. putting the pathogenic microorganism-containing medicament for control of red imported fire ants in a solid container, placing a corresponding amount of solution in a liquid container, then inserting the solid container into the liquid container, clamping a snap ring on a bottleneck of the liquid container, then pressing a rubber gasket to the snap ring, sleeving the rubber gasket and the snap ring with a cap and fixing the pieces to the bottleneck of the liquid container. The biopesticide preparationfor control of red imported fire ants provided by the invention has the characteristics of long storage time, convenient use and transportation, time saving and labor saving, and immediate usabilityafter preparation, and can improve the drug (spore) germination environment and increase the insecticidal effect after use, thus finally achieving the purpose of field control of red imported fire ants.

Description

Technical field: [0001] The invention belongs to the field of prevention and control of red imported fire ants, and in particular relates to a preparation method of a biological pesticide preparation for preventing and controlling red imported fire ants. Background technique: [0002] Beauveria bassiana: Beauveria bassiana is an ascomycete entomogenic fungus. The high sporopollen of Beauveria bassiana is non-toxic and tasteless, and has no environmental pollution. It has persistent infection power to pests, and once the pests are infected, they can spread continuously. No pesticide residue, even if the product is harvested immediately after the application of Beauveria bassiana, it will not cause any pesticide residues; no resistance, Beauveria bassiana is infected and killed by contact with the body wall of the pest under natural conditions, and the pest will not develop any resistance to it Re-growth, Beauveria bassiana new biopesticide contains living fungi and spores, af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N59/08A01P7/04A01N63/30
CPCA01N63/30A01N25/14A01N25/22A01N25/04A01N59/08
Inventor 李军韩诗畴叶静文押玉柯吕欣
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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