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Edible fungus mycelium detoxification kit and mycelium detoxification regeneration process

A technology of edible mushrooms and kits, applied in the direction of microorganisms, fungi, methods based on microorganisms, etc., can solve the problems of mushroom farmers’ loss, easy to open umbrellas, and decreased ability to resist Trichoderma, and achieve increased production, convenient use, and convenience The effect of promotion

Inactive Publication Date: 2018-01-26
冕宁万树食用菌产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, edible fungus viral diseases have appeared in some production areas in China, which have significantly affected the output and quality of edible fungi.
The test results show that dsRNA is ubiquitous in edible fungi, manifested as: reduced yield, reduced resistance to Trichoderma, many deformed mushrooms, fruiting bodies that are not resistant to carbon dioxide, and easy to open umbrellas
Even if the new varieties were selected, because the breeding unit did not track the stability of their genetic traits in time, many good varieties continued to be promoted for more than 3 years before virus diseases reappeared, resulting in serious degradation of the strains and causing great losses to the mushroom farmers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An edible fungus mycelia detoxification kit, which consists of the following systems: a liquid mycelium preparation system, an enzymatic hydrolysis system and a mycelium regeneration system,

[0027] Wherein, the liquid mycelium preparation system is composed of a liquid mycelium medium and a reagent sub-box loaded with the liquid mycelium medium. The liquid mycelium medium is composed of the following materials: 15 grams of glucose, 5 grams of peptone, yeast extract powder 4 grams, 1 gram of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 1000ml of water;

[0028] The enzymatic hydrolysis system consists of enzymatic hydrolysis solution and a reagent box loaded with enzymatic hydrolysis solution. The enzymatic hydrolysis solution is composed of the following components in mass percentage: 3% snail enzyme solution, 89% cellulase, and 8% wall-lyzing enzyme , wherein, the mass fraction of snail enzyme liquid is 1.5%, the mass fraction of cellulase is 2%, and ...

Embodiment 2

[0032] An edible fungus mycelia detoxification kit, which consists of the following systems: a liquid mycelium preparation system, an enzymatic hydrolysis system and a mycelium regeneration system,

[0033] Wherein, the liquid mycelium preparation system is composed of a liquid mycelium medium and a reagent sub-box loaded with the liquid mycelium medium. The liquid mycelium medium is composed of the following materials: 10 grams of glucose, 2 grams of peptone, yeast extract powder 6 grams, 0.5 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 1000ml of water;

[0034] The enzymatic hydrolysis system consists of enzymatic hydrolysis solution and a reagent box loaded with enzymatic hydrolysis solution. The enzymatic hydrolysis solution is composed of the following components in mass percentage: 8% snail enzyme solution, 85% cellulase, and 7% wall-lyzing enzyme , wherein the mass fraction of the snail enzyme solution is 1%, the mass fraction of the cellulas...

Embodiment 3

[0038] An edible fungus mycelia detoxification kit, which consists of the following systems: a liquid mycelium preparation system, an enzymatic hydrolysis system and a mycelium regeneration system,

[0039] Wherein, the liquid mycelium preparation system is composed of a liquid mycelium medium and a reagent sub-box loaded with the liquid mycelium medium. The liquid mycelium medium is composed of the following materials: 20 grams of glucose, 10 grams of peptone, yeast extract powder 1 gram, 2 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, 1000ml of water;

[0040] The enzymatic hydrolysis system consists of enzymatic hydrolysis solution and a reagent box loaded with enzymatic hydrolysis solution. The enzymatic hydrolysis solution is composed of the following components in mass percentage: 5% snail enzyme solution, 90% cellulase, and 5% wall-lyzing enzyme , wherein, the mass fraction of snail enzyme liquid is 0.5%, the mass fraction of cellulase is 3%, ...

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Abstract

The invention discloses an edible fungus mycelium detoxification kit and a mycelium detoxification regeneration process, wherein the process comprises the following steps: S1, liquid strain preparation; S2, mycelium preparation; S3, detoxification; S5, regeneration culture: coating a mycelium culture medium with an enzymatic hydrolysis solution, and at the culture temperature of 18-25 DEG C, culturing for 3-7 days until a bacterial colony appears; and S6, transferring the regenerated colony into a test tube to be used as a mother strain for standby application. The process has the beneficial effects that through strain detoxification, centrifugation and regeneration culture, detoxification treatment of various edible fungi mycelia and regeneration rejuvenation of the mycelia are achieved,the yield of edible fungi is improved, and the kit has the advantages of convenient operation; the adopted process can complete detoxification treatment of various edible fungi and strain regenerationrejuvenation within two weeks, and has the advantages of simple operation and convenient popularization.

Description

technical field [0001] The invention relates to the technical field of detoxification and regeneration of mycelium, in particular to a kit for detoxification of edible fungus mycelia and a process for detoxification and regeneration of mycelia. Background technique [0002] Edible mushrooms have a unique fragrance and multiple health functions. China's cultivation area and output rank first in the world. However, in recent years, edible fungus viral diseases have appeared in some production areas of China, which have significantly affected the yield and quality of edible fungi. The test results show that dsRNA is ubiquitous in edible fungi, which are manifested as: decreased yield, decreased resistance to Trichoderma, many deformed mushrooms, fruiting bodies that are not resistant to carbon dioxide, and easy to open umbrellas. Even if the new varieties were selected, because the breeding unit did not track the stability of their genetic traits in a timely manner, many excel...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 张守武
Owner 冕宁万树食用菌产业发展有限公司