Dentin-pulp complex preparation method
A dentin and composite technology, which is applied in prosthesis, medical science, tissue regeneration, etc., can solve the problem that the odontoblast layer, the mature dentin layer of the pre-dentin layer and the orderly and regular arrangement of the mature dentin layer cannot be realized, and the scaffold material is difficult to achieve. Problems such as pore size and pore size are formed to achieve the effect of saving experimental costs, improving repeatability, scientificity and innovation.
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[0036] Example 1 Preparation of dentin-pulp complex
[0037] Experiment name: Using natural dentin to induce odontogenic differentiation of bone marrow stromal cells in vivo to prepare dentin-pulp complexes
[0038] Experimental methods: natural dentin preparation, animal experiments, immunohistochemistry, PCR, Western blot
[0039] Experimental process: such as figure 1 As shown, in the present embodiment, the preparation method of dentin-pulp complex comprises the following steps:
[0040] 1) After the patient’s informed consent, 10 non-carious premolars extracted due to orthodontics in the outpatient department of the Stomatological Hospital were collected, the soft tissues were removed, cleaned and stored in a -80 °C refrigerator for later use;
[0041]2) Take out the sample teeth during preparation, cut off the enamel part of the crown on a low-speed hard tissue slicer, and keep the dentin layer from the top of the pulp cavity to the crown, with a thickness of 1.5 mm; ...
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