Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dentin-pulp complex preparation method

A dentin and composite technology, which is applied in prosthesis, medical science, tissue regeneration, etc., can solve the problem that the odontoblast layer, the mature dentin layer of the pre-dentin layer and the orderly and regular arrangement of the mature dentin layer cannot be realized, and the scaffold material is difficult to achieve. Problems such as pore size and pore size are formed to achieve the effect of saving experimental costs, improving repeatability, scientificity and innovation.

Inactive Publication Date: 2018-02-02
AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for these scaffold materials to form a suitable pore ratio and pore size to facilitate the entry and diffusion of cells and nutrients, as well as the removal of metabolic substances.
Moreover, the dentin pulp-like structure formed in vivo cannot realize the orderly arrangement of odontoblast layer, predentin layer and mature dentin layer.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dentin-pulp complex preparation method
  • Dentin-pulp complex preparation method
  • Dentin-pulp complex preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of dentin-pulp complex

[0037] Experiment name: Using natural dentin to induce odontogenic differentiation of bone marrow stromal cells in vivo to prepare dentin-pulp complexes

[0038] Experimental methods: natural dentin preparation, animal experiments, immunohistochemistry, PCR, Western blot

[0039] Experimental process: such as figure 1 As shown, in the present embodiment, the preparation method of dentin-pulp complex comprises the following steps:

[0040] 1) After the patient’s informed consent, 10 non-carious premolars extracted due to orthodontics in the outpatient department of the Stomatological Hospital were collected, the soft tissues were removed, cleaned and stored in a -80 °C refrigerator for later use;

[0041]2) Take out the sample teeth during preparation, cut off the enamel part of the crown on a low-speed hard tissue slicer, and keep the dentin layer from the top of the pulp cavity to the crown, with a thickness of 1.5 mm; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a dentin-pulp complex preparation method. The preparation method comprises the following steps: 1) after informed consent of a patient, collecting caries-free premolar teeth pulled out for orthodontic treatment in surgery departments of stomatological hospitals, removing soft tissues, washing clean and storing in -80 DEG C refrigerator for standby application; 2) in preparation, taking out a sample tooth, cutting a crown enamel part out on a low-speed hard tissue slicing machine and leaving and taking a dentin layer from the top of a pulp cavity to the crown, wherein thethickness is 1.5mm; 3) continuing cutting the obtained dentin into dentin slices with length * width * height as 2mm * 2mm * 2mm; 4) soaking dentin chips into in a 5 to 20%w / w EDTA solution for 10min, washing by PBS for 5min three times, soaking for 1min in a 19%w / w citric acid solution and washing by PBS for 5min three times. The dentin-pulp complex preparation method disclosed by the inventionhas the following beneficial effects: 1) a standardized preparation method can improve repeatability and scientificity of correlational researches; 2) the standardized preparation method can be used for detecting odotogenetic differentiation potential of seed cells in an experiment.

Description

technical field [0001] The invention relates to a preparation method of a tissue engineering complex, in particular to the preparation of a tissue engineering complex related to pulp regeneration, specifically a preparation method of a dentin-pulp complex. Background technique [0002] With the rapid development and wide application of tissue engineering technology, scaffold materials have also been improved and updated to varying degrees. In dental pulp tissue engineering, the clinical application of tissue engineered dental pulp can become more realistic by using porous scaffolds that can maintain the survival of stem cells and regenerate blood vessels and nerves. Because the scaffold can not only replace the basic mechanical and biological functions of the tissue, but also contain growth factors, cytokines and even DNA fragments that help stem cells proliferate and differentiate, thereby controlling and guiding the target tissue to develop better and faster. In teeth wit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/22A61L27/50
CPCA61L27/222A61L27/3633A61L27/3687A61L27/3834A61L27/3865A61L27/50A61L2430/12
Inventor 于金华雷港周洲张光东庄颖许涛闫明景双林
Owner AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products