Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of self-fluorescent polyacrylamide nanoparticle and its preparation method and application

A polyacrylamide and nanoparticle technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as weak penetrating imaging, achieve uniform particle size, good hydrophilicity, and simple preparation method handy effect

Active Publication Date: 2020-04-24
SOUTHEAST UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Visible fluorescence can only be used for in vitro biological detection and fluorescence imaging, but cannot be used for in vivo imaging of animals due to the strong fluorescence background of the animal itself and the weak tissue penetration of visible fluorescence excitation wavelengths

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of self-fluorescent polyacrylamide nanoparticle and its preparation method and application
  • A kind of self-fluorescent polyacrylamide nanoparticle and its preparation method and application
  • A kind of self-fluorescent polyacrylamide nanoparticle and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Dissolve 530mg of AA, 160mg of MBA, and 30mg of APMA in 2ml of ultra-pure water and mix well, and obtain a homogeneous solution by ultrasonication; then add 90mgε-PL to the solution and mix well to form an acrylamide monomer solution;

[0039] (2) Obtain polyacrylamide nanoparticles by inverse microemulsion method: first, 1.6g AOT and 3.1g Brij30 are added to a reaction flask containing 45mL of n-hexane, stirred for 40 minutes to form an oil phase; Add the acrylamide monomer solution prepared in step (1) dropwise, stir for 20 minutes, and finally add 80 μL of freshly prepared 100 mg / ml APS aqueous solution and 80 μL TEMED to initiate polymerization, and stir vigorously for 2 hours. After the polymerization is completed, remove n-hexane by rotary evaporation, and pass through absolute ethanol to precipitate the polymer particles, and use absolute ethanol to precipitate and wash the polymer several times to remove surfactants and residual monomers. After the ethanol is...

Embodiment 2

[0042] Example 2 has the same raw materials and preparation method as Example 1, except that 500mgAA, 150mg MBA, 20mg APMA, and 80mgε-PL are used in step (1); 1.4g AOT and 3.0g Brij30 are used in step (2) ; Step (3) glutaraldehyde solution consumption is 20 μ L.

Embodiment 3

[0044] Example 3 has the same raw materials and preparation method as Example 1, except that 550mgAA, 180mg MBA, 40mg APMA, and 100mgε-PL are used in step (1); 1.8g AOT and 3.2g Brij30 are used in step (2) ; Step (3) glutaraldehyde solution consumption is 120 μ L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an autofluorescent polyacrylamide nano particle and a preparation method and application thereof. The nano particle is obtained by cross-linking treatment of an acrylamide nanoparticle through glutaraldehyde, and the polyacrylamide particle is prepared from polylysine-containing acrylamide monomer solution. The prepared autofluorescent polyacrylamide nano particle can be efficiently internalized into cells to mark the cells, in-vitro and in-vivo long-time brightness and stability of autofluorescence are realized, great visible fluorescence and near infrared fluorescence properties are achieved, and particle size uniformity, high monodispersion, positive charges, high water absorbability, high hydrophilicity and remarkable biocompatibility in cells and animals are realized. In addition, the preparation method is simple, convenient, low in cost and extensive in raw material source, the prepared autofluorescent polyacrylamide nano particle can be applied to fieldsof biological detection, cell marking, animal imaging, medicine delivery, gene therapy and the like and has a significant application value.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a self-fluorescent polyacrylamide nanoparticle, a preparation method and application thereof. Background technique [0002] Currently, optical imaging methods are increasingly used for non-invasive, real-time and high-resolution disease detection. Optical imaging methods can obtain disease anatomical structure and even molecular information of disease metabolism from optical signals. Among various optical imaging technologies, fluorescence optical imaging technology has higher sensitivity and resolution. Light used for tissue imaging can be categorized into ultraviolet, visible, and near-infrared (NIR) light. NIR light (700-1000nm) is known as the "biological window" of the relatively low autofluorescence of the organism itself and its endogenous substances. NIR fluorescence (NIRF) has some key advantages over traditional visible fluorescence, including high sensitivity, high ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C08F220/56C08F222/38C08F220/60C08J3/24A61K49/00A61K47/32A61K48/00G01N21/64
CPCA61K47/32A61K48/0008A61K49/0019A61K49/0065C08F220/56C08J3/24C08J2333/26C09K11/06C09K2211/14G01N21/6428G01N2021/6439C08F222/385C08F220/60
Inventor 王进科谢红梅
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com