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Stable and high-specificity calcitonin detection kit

A technology for detecting kits and procalcitonin, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of low specificity, long detection time, high price, etc., and achieve high reaction sensitivity and good reagent stability Effect

Inactive Publication Date: 2018-02-02
山东诺安爱迪尔生物工程有限公司
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Problems solved by technology

The radioimmunoassay method uses artificially synthesized polyclonal antibodies to specifically recognize and link the synthetic amino acid procalcitonin. This method can detect serum procalcitonin in normal people, but it takes a long time to detect (19-22h), and Contaminated use of radioactive elements is restricted
Double-antibody sandwich immunochemiluminescence assay (ILMA) uses double monoclonal antibodies, one of which is a calcitonin antibody and the other is an anti-calcitonin antibody, which bind to the calcitonin and anti-calcitonin molecules of the procalcitonin molecule, respectively. This method is easy to operate and has strong specificity, but it is expensive, and it has great limitations in health institutions below the tertiary hospital
Colloidal gold colorimetric method (B.R.A.H.M.SPCT-Q-semi-quantitative rapid method) adopts colloidal gold technology, including colloidal gold-labeled anti-calcitonin monoclonal antibody and anti-calcitonin polyclonal antibody used as coating, the The method is fast, simple, and easy to observe, but it has the problem of low specificity, so it is only suitable for promotion in some relatively basic health institutions

Method used

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  • Stable and high-specificity calcitonin detection kit
  • Stable and high-specificity calcitonin detection kit
  • Stable and high-specificity calcitonin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Table 1 Example 1 reagent detection method

[0029]

[0030] Calculation: procalcitonin content (ng / mL) = (ΔA sample ÷ ΔA standard) × C standard.

[0031] Correlation experiment:

[0032] Utilize the formula of Example 1 to prepare the reagents, and carry out comparative detection with the procalcitonin assay kit of a company approved by the State Food and Drug Administration, which is common in the market, and detect 20 clinical serum samples at the same time, and the detection results are shown in Table 2. . And obtained the correlation curve of the two reagents (such as figure 1 Shown, x is embodiment 1 procalcitonin concentration, and y is the control group procalcitonin concentration), shows by testing result, the correlation coefficient (R 2 ) is 0.9996, indicating that there is a great correlation between the two. And when detecting low-value samples, compared with the control reagent, the probability of occurrence of 0 value is lower than that of the cont...

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Abstract

The invention relates to a stable and high-specificity calcitonin detection kit. A biclonal antibody is used and bonded to calcitonin and anti-calcitonin separately, and then coupled with latex microspheres to form antibody latex microsphere particles, so that the specificity is higher; surfactants, namely dodecyl trimethyl betaine and sodium alcohol ether sulphate, are used, so that the reagent stability is better; a 3-N-morpholine propanesulfonic acid (MOPS) buffer system is used, so that the reaction sensitivity of a reagent is higher.

Description

technical field [0001] The invention relates to a detection kit for clinical determination of procalcitonin in serum, belonging to the technical field of clinical in vitro detection. Background technique [0002] Serum procalcitonin is the propeptide substance of calcitonin. It is a glycoprotein with no hormone activity. The half-life in the human body is about 20-24 hours. It has good stability. The content of procalcitonin in normal human serum Very low, in addition to thyroid trauma, serum of patients with systemic inflammatory response syndrome (SIRS), sepsis, acute and chronic pneumonia, acute pancreatitis, active hepatitis, trauma, etc. is significantly elevated, especially for SIRS and sepsis, procalcitonin It is a very sensitive and specific serological marker. In the case of viral infection, the level remains low, and procalcitonin can increase in the early stage of severe bacterial infection (after 2-3 hours), so it has early diagnostic value. , graft host reject...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 刘振平赵峰玉
Owner 山东诺安爱迪尔生物工程有限公司
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