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Immune globulin G detection kit and detection method

An immunoglobulin and detection kit technology, which is applied in biological testing, measuring devices, material testing products, etc., can solve the problems of inaccurate test results of reagents, inability to meet the requirements for use, affecting the detection effect, etc., and achieve good stability of the reagents. , wide versatility, accurate detection results

Active Publication Date: 2017-12-15
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Antibodies are prone to flocculent or flaky precipitation during storage, resulting in poor antibody performance, poor repeatability, and large coefficient of variation (CV), directly resulting in inaccurate reagent test results, and additional filtration steps, complicated operation, And filtration may remove antibodies, affect the detection effect, cause certain impact on patients, and cannot meet the requirements for use

Method used

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  • Immune globulin G detection kit and detection method
  • Immune globulin G detection kit and detection method
  • Immune globulin G detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Immunoglobulin G detection kit

[0043] Reagent R1:

[0044] TRIS buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

polyethylene glycol 6000

1g / L

Ethylphenyl polyethylene glycol

0.9g / L

[0045] Reagent R2:

[0046] TRIS buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

Goat anti-human immunoglobulin G antibody

10mg / L

Ethylphenyl polyethylene glycol

0.9g / L

magnesium sulfate

0.01g / L

calcium acetate

0.01g / L

[0047] Calibrator:

[0048]Immunoglobulin G antigen standard diluent (20mmol / L phosphate buffer, 2g / L sodium chloride, 0.2g / L sodium azide, 0.3g / L dextran, 1g / L trehalose, 2g / L L sucrose, 2g / L bovine serum albumin) were dissolved, tested with a commercially available control reagent and adjusted to 120mg / L, subpackaged and stored at -20°C. Take it out before use, and dilute it with standard diluent...

Embodiment 2

[0049] Example 2 Immunoglobulin G detection kit

[0050] Reagent R1:

[0051] Phosphate buffer

40mmol / L

potassium chloride

7g / L

phenol

0.7g / L

polyethylene glycol 6000

30g / L

Ethylphenyl polyethylene glycol

51g / L

[0052] Reagent R2:

[0053] Phosphate buffer

40mmol / L

potassium chloride

5g / L

phenol

0.7g / L

Goat anti-human immunoglobulin G antibody

50mg / L

Ethylphenyl polyethylene glycol

51g / L

magnesium sulfate

15g / L

calcium acetate

22g / L

[0054] Calibrator:

[0055] Immunoglobulin G antigen standard diluent (50mmol / L glycine buffer, 10g / L sodium chloride, 0.5g / L sodium azide, 20g / L dextran, 25g / L trehalose, 30g / L sucrose , 20g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 100mg / L, and stored in -20°C. Take it out before use, and dilute it with standard diluent to obtain d...

Embodiment 3

[0056] Example 3 Immunoglobulin G detection kit

[0057] Reagent R1:

[0058] acetate buffer

80mmol / L

potassium chloride

20g / L

Sodium azide

0.8g / L

polyethylene glycol 6000

45g / L

Ethylphenyl polyethylene glycol

4.5g / L

[0059] Reagent R2:

[0060]

[0061]

[0062] Calibrator:

[0063] Immunoglobulin G antigen standard diluent (70mmol / L TRIS buffer, 25g / L sodium chloride, 1g / L sodium azide, 55g / L dextran, 50g / L trehalose, 60g / L sucrose, 75g / L bovine serum albumin) was dissolved, detected and adjusted to 200mg / L with a commercially available control reagent, subpackaged and stored at -20°C. Take it out before use, and dilute it with standard diluent to obtain different concentrations of immunoglobulin G standards (immunoglobulin G antigen concentration: 2mg / L, 10mg / L, 40mg / L, 60mg / L, 80mg / L). Then use a 0.65 μm filter membrane to filter and sterilize, and store at 2-8°C.

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PUM

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Abstract

The invention provides an immunonephelometry kit. The immunonephelometry kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains Nonidet P-40; and the reagent R2 contains Nonidet P-40, magnesium salt, calcium acetate and an antibody. The kit provided by the invention is good in antibody performance, good in repeatability and accurate in reagent detection result, and can meet the using requirement.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis of medical immunity, in particular to an immunoglobulin G detection kit and a detection method. Background technique [0002] Turbidimetry has been widely used in clinical testing. Now the most common application is immunoturbidimetry. [0003] Most of the early immunoassay techniques analyzed the presence and content of specific proteins in the sample to be tested by observing the formation of precipitates, the occurrence of agglutination and hemolysis, and measuring the light scattering caused by aggregates, such as immunodiffusion, Immunoelectrophoresis, direct and brief hemagglutination, passive hemagglutination, complement fixation test, etc. These detection methods are low in cost, easy to judge the results, easy to master technically, and can be widely used to detect various types of clinical samples. However, the above method tends to be eliminated due to its cumbersome operation, time-...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/82G01N21/31
CPCG01N21/31G01N21/82G01N33/6854G01N2021/825
Inventor 耿英利罗湘宇甘萍萍黎明龙腾镶
Owner SICHUAN MACCURA BIOTECH CO LTD
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