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A kind of human neural stem cell culture medium and application

A culture medium and stem cell technology, applied in the field of cell culture, can solve the problems of severe spontaneous differentiation and limited proliferation ability of human neural stem cells, and achieve the effect of easy differentiation and difficult long-term expansion, low cost, and clear chemical composition

Active Publication Date: 2020-08-25
BEIJING TRANSGEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the proliferation ability of human neural stem cells is limited in the existing medium, and the spontaneous differentiation is relatively serious.

Method used

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  • A kind of human neural stem cell culture medium and application
  • A kind of human neural stem cell culture medium and application
  • A kind of human neural stem cell culture medium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Human neural stem cell culture medium

[0033] A human neural stem cell culture medium comprising the following components:

[0034] Basal medium (volume ratio): 50% DMEM / F12 medium and 50% Neurobasal medium.

[0035] Nutritional additive: the composition and final use concentration of the nutritional additive are respectively: human insulin 10mg / L, vitamin C 50mg / L, glutathione 50mg / L, linolenic acid 1mg / L, carnitine 20mg / L, N-acetylcysteine ​​5μM, ethanolamine 5mg / L, linoleic acid 1mg / L.

[0036] Growth factor components and their final use concentrations: bFGF: 20ng / mL; EGF: 20ng / mL.

[0037]Signal pathway regulatory small molecule components and their final concentrations: Pifithrin-αhydrobromide, 5μM and 6-BIO, 2μM.

Embodiment 2

[0038] Example 2 Human neural stem cell culture medium

[0039] A human neural stem cell culture medium comprising the following components:

[0040] Basal medium (volume ratio): 50% DMEM / F12 medium and 50% Neurobasal medium.

[0041] Nutritional additive: the composition and final use concentration of the nutritional additive are respectively: human insulin 0.1mg / L, vitamin C 10mg / L, glutathione 10mg / L, linolenic acid 0.05mg / L, carnitine 0.2mg / L, N-acetylcysteine ​​5μM, ethanolamine 0.01mg / L, linoleic acid 0.05mg / L.

[0042] Growth factor components and their final use concentrations: bFGF: 5ng / mL; EGF: 5ng / mL.

[0043] Components of signaling pathway regulatory small molecules and their final concentrations: WNT3a, 1ng / mL and Baxinhibitor peptide V5, 0.1μM and Cyclic Pifithrin-αhydrobromide, 0.1μM.

Embodiment 3

[0044] Example 3 Human neural stem cell culture medium

[0045] A human neural stem cell culture medium comprising the following components:

[0046] Basal medium (volume ratio): 50% DMEM / F12 medium and 50% Neurobasal medium.

[0047] Nutritional additive: the composition and final use concentration of the nutritional additive are respectively: human insulin 20mg / L, vitamin C 200mg / L, glutathione 100mg / L, linolenic acid 5mg / L, carnitine 20mg / L, N-acetylcysteine ​​500μM, ethanolamine 10mg / L, linoleic acid 5mg / L.

[0048] Growth factor components and their final use concentrations: bFGF: 100ng / mL; EGF: 100ng / mL.

[0049] Components of signaling pathway regulatory small molecules and their final concentrations: WNT3a, 1ng / mL and Baxinhibitor peptide V5, 100μM and Cyclic Pifithrin-αhydrobromide, 100μM.

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Abstract

Provided is a human neural stem cell culture medium. The culture medium comprises the following components: a basal culture medium, a nutritional additive, a growth factor, and a signaling pathway-regulating small molecule. Also provided is an application of the culture medium in culturing human neural stem cells.

Description

technical field [0001] The present invention relates to the technical field of cell culture. More specifically, it relates to a human neural stem cell culture medium and application. Background technique [0002] The incidence of various neurological damage and degenerative neurological diseases is increasing year by year. Traditional medical methods are difficult to completely cure these diseases. Cell replacement therapy is the most effective means to completely cure these diseases. [0003] Neural stem cells have the ability to self-replicate and differentiate into cell types such as neural precursor cells, neurons, astrocytes, and oligodendrocytes. They are important tools for disease pathogenesis research, drug screening, etc. and ideal seed resource cells for cell replacement therapy of injury-like diseases. At present, there are many ways to obtain neural stem cells in vitro, such as primary isolation and culture from brain tissue, induction and differentiation of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2501/10C12N2501/11C12N2501/115C12N2501/40C12N2501/415C12N2501/48
Inventor 王娟马静辛文
Owner BEIJING TRANSGEN BIOTECH CO LTD