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Double-antibody sandwich method ELISA (Enzyme-linked Immuno Sorbent Assay) kit for diagnosing adult glioma

A glioma and double-antibody sandwich technology, applied in the field of biomedicine, can solve the problems of glioma dependence and inability to realize early warning, and achieve the effects of strong specificity, stable reagents, and improved sensitivity

Inactive Publication Date: 2018-02-06
GUANGDONG MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, the current diagnosis of glioma mostly relies on imaging techniques, which cannot achieve early warning

Method used

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  • Double-antibody sandwich method ELISA (Enzyme-linked Immuno Sorbent Assay) kit for diagnosing adult glioma
  • Double-antibody sandwich method ELISA (Enzyme-linked Immuno Sorbent Assay) kit for diagnosing adult glioma
  • Double-antibody sandwich method ELISA (Enzyme-linked Immuno Sorbent Assay) kit for diagnosing adult glioma

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Experimental program
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Effect test

Embodiment 1

[0046] 1. Experimental serum sample materials (see Table 1):

[0047] (1) 70 normal adult samples: Serum samples from normal physical examination population without metabolic diseases (diabetes, kidney disease), infectious diseases (HBV, HCV and HIV), and without any tumors tested by clinical laboratories.

[0048](2) 121 serum samples from glioma patients: including 20 in stage I, 27 in stage II, 43 in stage III and 31 in stage IV.

[0049] Table 1: Demographic and clinical data of normal adults and glioma patients

[0050]

[0051] 2. Double Antibody Sandwich ELISA Detection Kit of CCKBR

[0052] The kit contains:

[0053] (1) Human CCKBR recombinant monoclonal antibody coated on a solid-phase 96-well microtiter plate;

[0054] (2) The enzyme conjugate working solution is horseradish peroxidase (HRP)-labeled human CCKBR recombinant monoclonal antibody reaction solution;

[0055] (3) The positive control is human CCKBR standard protein, and the negative control is stan...

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Abstract

The invention discloses application of a blood serum CCKBR (Cholecystokinin B Receptor) to a glioma diagnosing marker and a double-antibody sandwich method ELISA (Enzyme-linked Immuno Sorbent Assay) kit for diagnosing glioma. The invention discloses a novel biomarker for diagnosing the glioma and especially has a very important effect and clinical application value on early warning and early diagnosis of the glioma. Meanwhile, the invention also establishes an enzyme-linked immuno diagnosing system based on a double-antibody sandwich principle and a glioma diagnosing kit is developed; detection of the CCKBR can be rapidly and accurately carried out and rapid auxiliary diagnosis can be carried out on primary glioma of each stage (especially an early stage); the kit has important clinical application value. The kit disclosed by the invention has the advantages of simplicity in operation, stable reagent, good repeatability, strong specificity, high sensitivity and the like and has a widemarket prospect of being easily popularized and applied in a large range.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to a double-antibody sandwich ELISA detection method and kit for diagnosing adult glioma. Background technique [0002] The term "glioma" refers to tumors originating from glial cells, including astrocytoma, oligodendroglioma, oligoastrocytoma, and ependymoma, among others. Glioma is the most common primary intracranial tumor. The WHO classification of tumors of the central nervous system divides gliomas into grades I to IV, in which grades I and II are low-grade gliomas, and grades III and IV are high-grade gliomas. grade glioma. Glioma can be pathologically diagnosed and classified according to tumor location, cell morphology, arrangement, differentiation characteristics, immunophenotype, ultrastructural and molecular genetic changes. [0003] Histological observation is still the basis of pathological diagnosis, but the molecular biological markers of gliom...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/577G01N33/574
CPCG01N33/535G01N33/574G01N33/577
Inventor 罗辉朱萧罗海清杨晓红黄永梅詹静婷雷金丽
Owner GUANGDONG MEDICAL UNIV
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