Inoculation method of culture mediums for hypsizygus marmoreus
A culture medium, seafood mushroom technology, applied in mushroom cultivation, botanical equipment and methods, cultivation and other directions, can solve the problems of large gap between bacteria and bacteria bag medium, low inoculation efficiency, uneven fruiting, etc., to reduce The probability of being contaminated, reducing the probability of uneven fruiting, and the effect of balanced planting depth
Active Publication Date: 2018-02-16
黑龙江泷安农业科技有限公司
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Problems solved by technology
The probability of contamination is high if the holes are drilled before inoculating the bacteria; the gap between the bacteria and the bacteria bag medium is large, the fruiting is irregular and the inoculation efficiency is low
Method used
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Embodiment 1
[0046] The inoculation of the seafood mushroom culture medium can be completed by adopting the following process steps:
[0047] (1) Put the culture material for cultivating seafood mushrooms into a plastic bag, tie the mouth of the bag tightly, and obtain the culture medium after sterilization, and set aside;
[0048] (2) Put multiple bamboo or wooden perforated inoculation sticks with multiple strain slots in the disinfection pot, boil the water in the pot for disinfection for 15-30 minutes, take it out and cool it naturally for later use;
[0049] (3) Insert the sterilized perforated inoculation rod into the seafood mushroom strain, so that the seafood mushroom strain is attached to the strain groove of the perforated inoculation rod;
[0050] (4) Insert the perforated inoculation stick with seafood mushroom strains into the medium in a linear shape from the surface of the medium. After insertion, the perforated inoculation stick cannot be taken out and left in the medium; ...
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Abstract
Disclosed is an inoculation method of culture mediums for hypsizygus marmoreus. The inoculation method comprises the steps that 1, culture materials for culturing hypsizygus marmoreus are loaded intoa plastic bag, an opening of the bag is tied, and after sterilization, the culture mediums are obtained for usage; 2, multiple perforated bamboo or wood inoculation rods with multiple strain grooves are disinfected for usage; 3, the perforated inoculation rods after the disinfection are inserted into hypsizygus marmoreus strains to make the hypsizygus marmoreus strains attached into the strain grooves of the perforated inoculation rods; 4, the perforated inoculation rods to which the hypsizygus marmoreus strains are attached are linearly inserted into the culture mediums from the surfaces of the culture mediums and cannot be taken out after insertion, so that the perforated inoculation rods stay in the culture mediums; 5, the culture mediums into which the perforated inoculation rods are inserted are stacked on a culture rack, the surfaces, where the perforated inoculation rods are inserted, of the culture mediums face upwards, and therefore inoculation is completed. Raw materials of the culture materials include tea tree branches, mulberry branches, tung tree branches, peanut shells, corncob residues, sugarcane residues, waste molasses and lime powder. The method is high in inoculation efficiency and capable of reducing the possibility of inoculation contamination of the strains.
Description
technical field [0001] The invention relates to the technical field of seafood mushroom cultivation, in particular to an inoculation method of a seafood mushroom culture medium. Background technique [0002] Seafood mushroom scientific name [ Hypsizygus marmoreus (Peck) H.E. Bigelow ], also known as jade mushroom, spot jade mushroom, true ji mushroom, crisp and tender in texture, fresh in taste, with a sea crab flavor, it is called "crab-flavored mushroom" and "seafood mushroom" in Japan. It belongs to the genus Bleomycetaceae and the genus Amygium, with medium to large fruiting bodies. Nowadays, there are two strains of light gray and pure white cultivated. The white strain is also called "white jade mushroom" and "yulong mushroom", and most of them are cultivated in factories. Cap diameter 3-15cm. From late summer to autumn, it grows in clusters on the dead wood and fallen rotten wood of broad-leaved trees. It is an excellent edible fungus in the northern temperate zon...
Claims
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IPC IPC(8): A01G18/00A01G18/20C05G1/00
CPCC05D3/02C05F5/00C05F5/002C05F11/00C05F11/08
Inventor 黄兴根
Owner 黑龙江泷安农业科技有限公司