Wikerhamomyces anomalus BT-1-1 and application thereof

A wickham and yeast technology, which is applied in the field of application of the abnormal Wickham yeast BT-1-1 and the abnormal Wickham yeast BT-1-1, and can solve problems such as unreported prevention and control of heavy metals and the like.

Active Publication Date: 2018-02-16
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the abnormal Wickhamian yeast has no

Method used

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  • Wikerhamomyces anomalus BT-1-1 and application thereof
  • Wikerhamomyces anomalus BT-1-1 and application thereof
  • Wikerhamomyces anomalus BT-1-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: the preparation of microbial preparation of the present invention

[0117] The implementation steps of this embodiment are as follows:

[0118] A. Activation of bacteria

[0119] The abnormal Wickham yeast BT-1-1 preserved by picking a ring of glycerol was inoculated in the wort medium sold by Guangdong Huankai Microbial Technology Co., Ltd. under the trade name Malt Extract Medium, at temperature Under the conditions of 30°C and 150r / min, the shaker was cultured in a shaker sold by Shanghai Yiheng Scientific Instrument Co., Ltd. under the trade name THZ-103B for 24 hours to obtain the first-generation shaker culture; the national standard GB 4789.15- 2010 food microbiology inspection mold and yeast count analysis method detection, the first generation shaker culture contains 4 ~ 5 × 10 6 CFU / mL Abnormal Wickham yeast BT-1-1 live cells.

[0120] B. Expanded culture of yeast

[0121] According to the 2% inoculum amount based on the volume of the YEPD me...

Embodiment 2

[0131] Embodiment 2: the application of microbial preparation of the present invention in the production of kumiss fermented food

[0132] The implementation mode of this embodiment is as follows:

[0133] According to the kumiss production process disclosed in CN 101260358 B, in the first fermentation step, the microbial preparation of the present invention is added in an amount of 0.4%, to obtain the kumiss fermented food.

[0134] According to GB14923-2001 national standard for experimental animals, the test results show that feeding the kumiss fermented food can reduce the levels of lead and cadmium in the blood of rats to 37.7% and 46.7% respectively of the unintervened group.

Embodiment 3

[0135] Embodiment 3: the application of microbial preparation of the present invention in the production of fermented pig feed

[0136] The implementation mode of this embodiment is as follows:

[0137] According to the fermented feed production process disclosed in CN 102228129 B, the microbial preparation of the present invention is added in the semi-solid fermentation step according to 0.5% to obtain the pig fermented feed,

[0138] According to GB14923-2001 national standard for experimental animals, the test results show that feeding the fermented pig feed can reduce the levels of lead and cadmium in blood to 43.3% and 56.7% respectively of the unintervened group.

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Abstract

The invention relates to wikerhamomyces anomalus BT-1-1 and an application thereof. The wikerhamomyces anomalus BT-1-1 provided by the invention is used for preparing highly tolerant and highly adsorptive heavy metal lead and cadmium saccharomycetes microbial preparation. The wikerhamomyces anomalus BT-1-1 is prepared from the following steps: culture activation, saccharomycetes enlage cultivation, centrifugal separation, protecting agent preparation, protecting agent addition, freezing and drying and the like. The highly tolerant and highly adsorptive heavy metal lead and cadmium saccharomycetes microbial preparation has an efficient removal ability on heavy metals lead and cadmium, and is used as a fragrance producing strain for producing wine, feeds and the like, and is safe and reliable to eat.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a Wickerhamomyces anomalus BT-1-1, and also relates to the use of Wickerhamomyces anomalus BT-1-1. 【Background technique】 [0002] Although heavy metal pollution is not a recent phenomenon, it is still the most important problem in environmental pollution endangering human health. At present, for the acute poisoning of lead and cadmium in humans and animals, mercapto compounds such as dimercaptopropanol (BAL) and dimercaptopropanesulfonate (DMPS) are often taken for detoxification, but the chronic poisoning and accumulation of lead and cadmium can be alleviated. Still no proven method. Yeast is a widely used and safe microorganism in food fermentation, and it is easy to cultivate and reproduce quickly. More and more reports have found that it has good tolerance and adsorption capacity for heavy metals lead and cadmium, but it is not suitable for...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23L33/14A23K10/12C12R1/645
CPCA23V2002/00A23K10/12A23L33/14C12N1/16C12N1/145C12R2001/645A23V2200/30A23V2250/76
Inventor 贺银凤刘亚东田建军王苏和贺敏李丽杰
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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