A kind of abnormal Wickham's yeast bt-1-1 and its use
A Wickham and abnormal technology, applied in the field of application of Wickham abnormal yeast BT-1-1, abnormal Wickham yeast BT-1-1, can solve the problems that have not been reported in the prevention and treatment of heavy metals
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Embodiment 1
[0116] Embodiment 1: the preparation of microbial preparation of the present invention
[0117] The implementation steps of this embodiment are as follows:
[0118] A. Activation of bacteria
[0119] The abnormal Wickham yeast BT-1-1 preserved by picking a ring of glycerol was inoculated in the wort medium sold by Guangdong Huankai Microbial Technology Co., Ltd. under the trade name Malt Extract Medium, at temperature Under the conditions of 30°C and 150r / min, the shaker was cultured in a shaker sold by Shanghai Yiheng Scientific Instrument Co., Ltd. under the trade name THZ-103B for 24 hours to obtain the first-generation shaker culture; the national standard GB 4789.15- 2010 food microbiology inspection mold and yeast count analysis method detection, the first generation shaker culture contains 4 ~ 5 × 10 6 CFU / mL Abnormal Wickham yeast BT-1-1 live cells.
[0120] B. Expanded culture of yeast
[0121] According to the 2% inoculum amount based on the volume of the YEPD me...
Embodiment 2
[0131] Embodiment 2: the application of microbial preparation of the present invention in the production of kumiss fermented food
[0132] The implementation mode of this embodiment is as follows:
[0133] According to the kumiss production process disclosed in CN 101260358 B, in the first fermentation step, the microbial preparation of the present invention is added in an amount of 0.4%, to obtain the kumiss fermented food.
[0134] According to GB14923-2001 national standard for experimental animals, the test results show that feeding the kumiss fermented food can reduce the levels of lead and cadmium in the blood of rats to 37.7% and 46.7% respectively of the unintervened group.
Embodiment 3
[0135] Embodiment 3: the application of microbial preparation of the present invention in the production of fermented pig feed
[0136] The implementation mode of this embodiment is as follows:
[0137] According to the fermented feed production process disclosed in CN 102228129 B, the microbial preparation of the present invention is added in the semi-solid fermentation step according to 0.5% to obtain the pig fermented feed,
[0138] According to GB14923-2001 national standard for experimental animals, the test results show that feeding the fermented pig feed can reduce the levels of lead and cadmium in blood to 43.3% and 56.7% respectively of the unintervened group.
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