SNP marker combination and identification method of Xiaomeishan pig and raw meat products

An identification method and meat product technology are applied in the field of SNP marker combination and identification of Xiaomeishan pigs and raw meat products to achieve the effect of simple and quick operation.

Active Publication Date: 2021-06-29
SHANGHAI JIAOTONG UNIV
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Problems solved by technology

[0003] In view of the above-mentioned deficiencies in the prior art, the present invention proposes a combination of SNP markers and an identification method for identifying Xiaomeishan pigs and raw meat products, using third-generation molecular marker identification and Sanger sequencing technology to identify Xiaomeishan pigs and raw meat products, solving the problem There is no identification method related to Xiaomeishan pig and its meat products in the art, and a method and related special primers for identifying Xiaomeishan pig and its meat products with accurate results, simple operation and low price are provided

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  • SNP marker combination and identification method of Xiaomeishan pig and raw meat products
  • SNP marker combination and identification method of Xiaomeishan pig and raw meat products

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Embodiment Construction

[0054] Twenty ear tissue samples of Xiaomeishan pigs were selected for identification, and 10 ear tissue samples of each of the other twelve breeds (strains) were selected, and tissue DNA was extracted by the improved SDS method.

[0055] Using primer pairs C12, C6, C16, C71, and C79, PCR reaction amplification was performed.

[0056] The reaction system of PCR reaction is 10uL system: template DNA 1ng / uL, primer 1uL, H 2 O 3.8uL, 2×TaqPCR Masrer Mix 50uL; and / or, the reaction program of PCR reaction is pre-denaturation at 95°C for 2min, pre-denaturation at 95°C for 30s, annealing temperature at 60°C for 30s, extension at 72°C for 1min, cycle times 30 times, 72 °C for another 10 min.

[0057] Use 2% agarose gel and 1×TAE buffer as the medium to electrophoresis to detect the amplification results. The gel electrophoresis conditions are current 10A, voltage 100 volts, and time 40 minutes. The gel electrophoresis pattern of the amplification product of the C12 primer appears f...

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Abstract

A SNP marker combination and identification method for Xiaomeishan pigs and raw meat products, by extracting the genomic DNA of raw pork or meat products, performing agarose gel electrophoresis and Sanger sequencing after PCR amplification, and according to the SNP at the characteristic site of the sequencing results Genotype identification of Xiaomeishan pig and its meat products; the Sanger sequencing, its identification sites are: specific mutations appear at SNP6, SNP12, SNP16, SNP71, and SNP79 sites; Issues related to identification methods for pigs and their meat products.

Description

technical field [0001] The invention relates to a technology in the field of food safety monitoring, in particular to a SNP marker combination and identification method for Xiaomeishan pigs and raw meat products. Background technique [0002] Meishan pig is one of the excellent local breeds (strains) in the Taihu Lake Basin in my country. It is famous for its high fecundity and delicious meat. With the development of sequencing technology, molecular markers have also developed from restriction fragment length polymorphism (RFLP) in the first generation, variable number tandem repeat polymorphism (SSR) in the second generation, to single nucleotide polymorphism ( SNP). Compared with the previous two generations of molecular markers, the third-generation molecular marker SNP has the advantages of rich variation, low requirement for DNA samples, high stability, accurate determination, simple detection method and high throughput. At present, the third-generation molecular mark...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6888C12Q2600/156C12Q2535/101
Inventor 潘玉春岳阳王起山张哲徐忠孙浩刘登英赵伟马裴裴
Owner SHANGHAI JIAOTONG UNIV
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