IV-type collagen detection kit and application method thereof

A detection kit and collagen technology, applied in the field of medical testing, can solve the problems of radioactive contamination, complicated operation of radioimmunoassay methods, and high investment, and achieve the effects of low cost, high detection sensitivity and accuracy, and good stability.

Active Publication Date: 2018-02-16
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for detecting type IV collagen is mainly the radioimmunoassay method, but the specific operation of the radioimmunoassay method is relatively cumbersome, and there are certain problems such as radioactive pollution and high investment.

Method used

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  • IV-type collagen detection kit and application method thereof
  • IV-type collagen detection kit and application method thereof
  • IV-type collagen detection kit and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A type IV collagen detection kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0052] Reagent R1:

[0053]

[0054] The solvent is purified water;

[0055] Reagent R2:

[0056]

[0057] The solvent is purified water;

[0058] The raw material components of the type IV collagen polyclonal antibody coated with latex are: latex microspheres, type IV collagen polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein polyethylene p-chloromethyl styrene The content of ethylene copolymer in the overall reagent R2 was 9%.

Embodiment 2

[0060] A type IV collagen detection kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0061] Reagent R1:

[0062]

[0063] The solvent is purified water;

[0064] Reagent R2:

[0065]

[0066] The solvent is purified water;

[0067] The raw material components of the type IV collagen polyclonal antibody coated with latex are: latex microspheres, type IV collagen polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein polyethylene p-chloromethyl styrene The content of ethylene copolymer in the overall reagent R2 was 11%.

Embodiment 3

[0069] A type IV collagen detection kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0070] Reagent R1:

[0071]

[0072] The solvent is purified water;

[0073] Reagent R2:

[0074]

[0075] The solvent is purified water;

[0076] The raw material components of the type IV collagen polyclonal antibody coated with latex are: latex microspheres, type IV collagen polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein polyethylene p-chloromethyl styrene The content of ethylene copolymer in the overall reagent R2 was 10%.

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Abstract

The invention discloses an IV-type collagen detection kit, which includes a reagent R1 including a MOPSO buffer, NaCl, NaN3, Arabic gum, tween-80, and PEG-2000, with the solvent being purified water;and a reagent R2 including a MOPSO buffer, NaCl, NaN3, BSA, glycerin, and an IV-type collagen polyclonal antibody coated with latex, with the solvent being purified water, wherein the IV-type collagenpolyclonal antibody coated with latex is made from latex microspheres, an IV-type collagen polyclonal antibody, and ethylene and 4-vinylbenylchloride copolymer. The invention further discloses an application method of the IV-type collagen detection kit. The kit has the advantages that (1) the kit is high in detection sensitivity and accuracy, and is simple and fast to operate; (2) the formed antigen-antibody compound is excellent in stability and good in specificity; (3) the kit is low in cost and free of pollution; and (4) the kit can be used for a fully-automatic biochemical analyzer.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a type IV collagen detection kit and a use method thereof. Background technique [0002] Collagen is a fibrous glycoprotein consisting of a network of α-peptide chains formed by triple helices. At present, as many as 10 kinds of collagen have been found, which exist in different tissues. Type IV collagen is an important component of the basement membrane. The normal intrahepatic basement membrane mainly exists around blood vessels, lymphatic vessels, and bile ducts, and is absent in the sinusoidal wall of the liver. With the development of inflammation in liver disease, fibrous tissue hyperplasia is active, a large amount of collagen is deposited in the process of fibrous tissue formation, and various collagens are increased, but the most important one is the increase of type IV collagen that constitutes the basement membrane. At present, it is believed that the determ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/544
CPCG01N33/54313G01N33/544G01N33/6803G01N2333/78
Inventor 吴铮丁先骏吴泽东庄庆华张金东
Owner ANHUI IPROCOM BIOTECH CO LTD
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