A mercury ion fluorescence detection kit

Abstract

The invention discloses a mercury ion fluorescence detection kit. A T-T mismatching base-containing nucleic acid is used as a recognition unit to construct a novel mercury ion detection technology free of an antibody and an enzyme. In detection, washing separation is avoided and detection is finished through direct mixing. The kit has the advantages of simple operation and low cost. A stem-loop structure nucleic acid and mercury ions are bonded and then the later DNA fulcrum-mediated strand displacement reaction is further initiated so that nucleic acid modified with a quenching group is replaced and the fluorescence group-labeled nucleic acid produces fluorescence and thus detection is realized. The system has a mercury ion detection limit of 0.1 nM and satisfies the actual sample detection demands. Common other ions do not influence detection and good specificity is obtained.

Description

technical field [0001] The invention relates to the detection field of environmental pollutants, in particular to a detection kit for heavy metal ions, in particular to a fluorescence detection kit for mercury ions. Background technique [0002] Mercury ion (Hg 2+ ) is a widely distributed and highly toxic pollutant with strong carcinogenicity, and will cause great harm to the environment and public health even at extremely low concentrations. Among them, mercury pollution from polluted waste water, electronics industry, plastics industry, mercury mixing and metallurgy and other industries is the most harmful. The harm of mercury to the human body mainly involves the central nervous system, digestive system and kidneys, and also has certain effects on the respiratory system, skin, blood and eyes. Therefore, daily monitoring of mercury in soil, air, water and other environments and food is of great significance. According to the US Environmental Protection Agency EPA regul...

AI Technical Summary

Problems solved by technology

However, these techniques usually require complex operations and sample pretreatment processes as well as expensive instruments, which seriously restrict the application of these detection methods.

Reported Hg 2+ Most of the detection techniques and kits involve the use of antibodies and enzymes, which require multiple washing and separation processes. The procedures are cumbersome, time-consuming and labor-intensive. Therefore, it is urgent to establish a detection technology and detection kits that do not require antibodies and enzymes, and avoid the washing and separation process. of great significance

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