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Portable outdoor spore separator and spore separation acquisition method

A spore separation and portable technology, which is applied in the methods of using spores, biochemical equipment and methods, and methods of sampling biological materials, etc. It is not easy to preserve and other problems, and achieves the effect of easy promotion, simple structure and strong practicability

Active Publication Date: 2018-02-27
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the investigation of Entomomycetes fungi often requires that the infected aphid corpses be brought back to the laboratory for isolation and identification. In addition, Entomomycetes fungi will eject conidia in a short period of time, and conidia are not easy to preserve
Aseptic operation cannot be guaranteed outdoors, so the current traditional method of relying on laboratory ultra-clean workbench separation cannot be applied to outdoor collection, which brings great obstacles to the in-depth study of Entomycetes fungi

Method used

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  • Portable outdoor spore separator and spore separation acquisition method
  • Portable outdoor spore separator and spore separation acquisition method
  • Portable outdoor spore separator and spore separation acquisition method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 A kind of structure of portable spore separator

[0053] Such as Figures 1 to 12 The shown portable outdoor spore separator includes a disinfection tank 1, a lower valve disc 2, a middle valve disc 3 and an upper cover disc 4, which are detachably assembled together from bottom to top.

[0054] Disinfection tank 1 is used to hold volatile disinfection and sterilization liquid or sterile water, and is located at the bottom of the separator as a supporting device of the portable outdoor spore separator.

[0055] The lower valve disc 2 is a cylinder and is provided with a through hole of a certain diameter at the position of the sextile radiation on the circular surface 21 of the cylinder. Three of the through holes are sterilizing holes 22, and the sterilizing holes can be used as a volatilization passage for the volatile sterilizing liquid to collect biological samples (such as aphid corpses) and the lower valve disc and the middle valve disc installed on ...

Embodiment 2

[0068] Embodiment 2 Utilizes the method for collecting spores by the portable spore separator described in embodiment 1

[0069] Take the collection of Entomycetes fungal spores as an example for specific illustration. All operations must be performed while wearing masks and disposable rubber gloves.

[0070] Use 75% alcohol or other sterilizing solution to firstly carry out disinfection treatment to components such as the lower valve disc 2, the middle valve disc 3, and the upper cover disc 4.

[0071] A certain amount of volatile sterilizing liquid is packed into disinfection tank 1, obtain susceptible aphids with a sterilized sampling stick (for example, get the susceptible aphids with a writing brush that has been sprayed with 75% alcohol), and the susceptible aphids (aphids) body) is fixed in the sample storage groove 43 at the top spacer in the upper cover tray. The method of immobilizing the susceptible aphids can be achieved by means of double-sided tape or the adhes...

Embodiment 3

[0078] The comparative experiment of embodiment 3 sporulation quantity

[0079] The comparative test of the sporulation number index adopts the two collection methods of portable spore separator collection and cryopreservation tube collection described in Example 1, and counts the sporulation number in the two collection methods. Statistical methods are used to analyze the difference in the number of spores in the two modes, and then determine the feasibility of the collection mode of the outdoor spore separator of the present invention. Select 30 susceptible aphids as test objects for statistical analysis, that is, for each of the two methods, randomly select 30 susceptible aphids of similar size for the test.

[0080] Utilize the spore separator described in Example 1 and the installation method of each plate of Example 2, the susceptible aphids collected are fixed in the sample storage tank in the upper cover plate of the separator with double-sided tape, and the concave bo...

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PUM

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Abstract

The invention discloses a portable outdoor spore separator and a spore separation acquisition method. The portable outdoor spore separator at least comprises a sterilization tank, an upper cover discand a collecting hole, wherein the collecting hole is formed inside the separator; the sterilization tank is covered by the upper cover disc; the upper opening of the sterilization tank is a liquid volatilization hole; a sample storing groove is formed in the upper cover disc; the sample storing groove is positioned above the volatilization hole or the collecting hole. By adopting the separator and the separation method disclosed by the invention, fungal spores can be separated under a sterile condition, the equipment requirement that a conventional method needs to be implemented on an ultra-clean working table is avoided, the portable outdoor spore separator is convenient to carry over, small in size and easy to operate, and convenience can be brought to outdoor pathogenic fungus collection.

Description

technical field [0001] The invention relates to the field of separation and collection of strains, in particular to a spore separation collector and collection method suitable for unclean environments. Background technique [0002] In the research process of fungi, the isolation and expansion of strains are very important. Strain isolation is to separate the target fungi from the mixed microorganisms, and then purify and cultivate the isolated target bacteria in a suitable environment. In order to avoid contamination, the separation work is required to be carried out under sterile conditions. At present, isolation culture needs to be carried out in a sterile room, a sterile box or a sterile workbench (ultra-clean workbench). Sterile rooms and aseptic boxes should be dedusted by spraying and disinfected with drugs or ultraviolet radiation. Commonly used disinfectants are sprays such as 70% alcohol, 2% creol soap, and 5% carbolic acid. It takes 20-30 minutes to irradiate wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12M1/26C12M1/12
CPCC12M33/00C12M37/00C12N3/00
Inventor 陈春胡亚强彭锐
Owner CHINA JILIANG UNIV