Method for bacterially degumming corn bran fibers
A degumming method and technology of corn husks, applied in the field of microbial degumming of corn husk fibers, can solve problems such as fiber damage and environmental pollution, and achieve the effects of reducing damage, reducing fiber damage, and low fiber residual glue rate
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[0028] (3) Preparation of corn husk fiber microbial degumming solution, aspergillus niger (Aspergillus niger) HYA4 is cultivated in a solid fermentation medium, the fermented product is extracted and centrifuged to obtain a supernatant as a degumming solution;
[0029] The Aspergillus niger HYA4 has a preservation number of CGMCC No.1897;
[0030] The described solid fermentation medium and culture conditions are: bran:straw=3:2, ammonium sulfate addition is 5.5%, material-to-water ratio is 1:3, initial pH is 5.5, culture temperature is 32~36 DEG C, culture The period is 72h;
[0031] The preparation method of the degumming solution is as follows: add water to Aspergillus niger (Aspergillus niger) HYA4 solid fermentation culture for 1.5h with a water ratio of 1:20, and then centrifuge, the centrifugal speed is 3000r / min, and the centrifugation time is 10min;
[0032] Before the described Aspergillus niger (Aspergillus niger) HYA4 solid fermentation, the strain preservation st...
Embodiment 1
[0058] (1) select corn husks without mildew, and remove dust and debris on the corn husks;
[0059] (2) Carry out soaking pretreatment to corn husk fiber, the bath ratio is 1:10 (w / v), the concentration of pulling apart powder BX is 2g / L, and treatment time is 4h;
[0060] (3) Inoculate Aspergillus niger (Aspergillus niger) HYA4 on a solid fermentation medium, the composition of the medium is bran: straw = 3:2, the amount of ammonium sulfate added is 5.5%, the ratio of material to water is 1:3, and the initial pH is 5.5 After cultivating at 36°C for 72 hours, the fermentation was completed, and water was added to the solid fermentation culture of Aspergillus niger (Aspergillus niger) HYA4 for 1.5 hours with a water ratio of 1:20, and then centrifuged at a speed of 3000r / min, and the centrifugation time was 10min, the resulting supernatant was used as a degumming solution;
[0061] (4) Microbial degumming of corn bran fiber, place the corn bran fiber after step (2) pretreatmen...
Embodiment 2
[0065] (1) select corn husks without mildew, and remove dust and debris on the corn husks;
[0066] (2) Carry out soaking pretreatment to the corn husk fiber, the bath ratio is 1:15 (w / v), the BX concentration of pulling apart powder is 1.5g / L, and the treatment time is 5h;
[0067] (3) Inoculate Aspergillus niger (Aspergillus niger) HYA4 on a solid fermentation medium, the medium composition is bran: straw=3:2, the amount of ammonium sulfate added is 5.5%, the ratio of material to water is 1:3, and the initial pH is 5.5 After cultivating at 36°C for 72 hours, the fermentation was completed, and water was added to the solid fermentation culture of Aspergillus niger (Aspergillus niger) HYA4 for 1.5 hours with a water ratio of 1:20, and then centrifuged at a speed of 3000r / min, and the centrifugation time was 10min, the resulting supernatant was used as a degumming solution;
[0068] (4) Microbial degumming of corn husk fiber, place the pretreated corn husk fiber in step (2) in...
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