A method for extracting anthocyanins

An anthocyanin and extraction technology, applied in organic chemistry and other directions, can solve the problems of anthocyanin degradation physiological activity, anthocyanin toxicity residue, low production efficiency, etc., and achieves wide practical application field, high economic value, and production cost saving Effect

Active Publication Date: 2021-08-06
SHENZHEN SHENBAO HUACHENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anthocyanins extracted by traditional organic solvents often have toxic residues, and the production process has a lot of environmental pollution, long extraction time, low production efficiency, and hot solvents are likely to cause anthocyanins to degrade and reduce physiological activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In this example, roselle is used as a raw material to extract anthocyanins. The specific methods include:

[0028] 1) chopping: chopping the dried roselle flowers to 5-10 meshes with a tea chopper;

[0029] 2) Enzymatic extraction: put the minced roselle into an enzymatic hydrolysis tank, add pure water at 48°C, the ratio of roselle raw material to pure water is 1:25, add compound enzyme preparation according to 5% of the weight of roselle raw material , enzymolysis time 2h; after enzymolysis reaches the end point, open the discharge valve, enzymolysis liquid is squeezed into vibrating screen and carry out coarse filtration; The compound enzyme preparation of this example is cellulase: pectinase weight ratio is 1:2 Compound enzyme;

[0030] 3) Coarse filtration: use a double-layer vibrating screen, the upper layer is 80 mesh, and the lower layer is 200 mesh to remove some large impurities;

[0031] 4) Cooling: Cool the filtrate of the coarse filtration to 15°C and the...

Embodiment 2

[0042] In this example, roselle is used as a raw material to extract anthocyanins. The specific methods include:

[0043] 1) chopping: chopping the dried roselle flowers to 5-10 meshes with a tea chopper;

[0044]2) Enzymatic extraction: put the minced roselle into an enzymatic hydrolysis tank, add pure water at 52°C, the ratio of roselle raw material to pure water is 1:20, add compound enzyme preparation according to 3% of the weight of roselle raw material , Enzymolysis time 2h. After the enzymolysis reaches the end point, open the discharge valve, and the enzymolysis solution is squeezed into a vibrating screen for coarse filtration; the compound enzyme preparation of this example is a cellulase: a compound enzyme with a pectinase weight ratio of 1:2;

[0045] 3) Coarse filtration: use a double-layer vibrating screen, the upper layer is 80 mesh, and the lower layer is 200 mesh to remove some large impurities;

[0046] 4) Cooling: Cool the filtrate of the coarse filter to ...

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PUM

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Abstract

The application discloses a method for extracting anthocyanins. The method for extracting anthocyanins in this application includes using roselle as a raw material, chopping the raw material to 5-10 mesh, and using pure water and adding a compound enzyme preparation to enzymatically process the chopped raw material under the condition of 45-55°C. Extraction, the extract is sequentially centrifuged, ultrafiltered, concentrated by reverse osmosis, purified by macroporous adsorption resin, and freeze-dried to obtain high-purity anthocyanins; the compound enzyme preparation includes cellulase and pectinase. The anthocyanin extraction method of the present application has a simple process and is easy to operate, and has a high extraction rate of anthocyanin, relatively saves production costs, and has higher economic value. The anthocyanins extracted by the method of the application have a purity of more than 70%, so that the extracted anthocyanins have higher medical value and wider practical application fields. Moreover, the method of the present application avoids organic solvent residues, reduces environmental pollution, and has better social benefits.

Description

technical field [0001] The present application relates to the field of anthocyanin extraction, in particular to a method for extracting anthocyanin. Background technique [0002] Anthocyanins (Anthocyanidins), which belong to flavonoids in phenolic compounds, are water-soluble pigments. Widely present in the tissues of plant petals and fruits, as well as the surface cells and lower epidermis of stems and leaves. Its color changes with pH, ​​which gives many plants their bright, vibrant colors. [0003] In the natural state, anthocyanins are often combined with various monosaccharides to form glycosides in plants, called anthocyanins (Anthocyanin), which was proposed by Marguart when he named the blue extract from cornflower flowers , is now used as a general term for similar substances. [0004] Anthocyanins widely exist in flowering plants, especially angiosperms, and their content in plants varies greatly with varieties, seasons, climates, and maturity. According to pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 周军罗龙新何群仙
Owner SHENZHEN SHENBAO HUACHENG TECH
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