Grifola frondosa facility cultivation new variety and its liquid fermentation strain production method
A technology of liquid fermentation and new varieties, applied in botany equipment and methods, biochemical equipment and methods, methods based on microorganisms, etc., can solve the problems of long cultivation period, tight market, high price, etc., achieve short cultivation period, The effect of low cost and short cycle
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Embodiment 1
[0105] Embodiment 1 The preparation method of the liquid fermentation strain of the new variety of Grifola frondosa frondosa facility cultivation of the present invention
[0106] Grifola frondosa solid cultivation has long fruiting body growth cycle, low yield and instability; liquid fermentation culture has the advantages of short cycle, low cost, high yield, etc. It is immature, the cultivation period is long, and the mycelial properties are uneven, which are the limiting factors for the cultivation of Grifola frondosa with liquid strains.
[0107] The present invention develops a liquid fermentation method for the new variety "Jihui 0161" of Grifola frondosa, and obtains a set of fermentation methods suitable for the new variety "Jihui 0161". The specific steps are as follows:
[0108] (1) Proliferation of solid stock culture medium
[0109] (1) Preparation and distribution of solid stock culture medium
[0110] Solid stock medium formula: potato 200g / L, glucose 20g / L, a...
experiment example 1
[0143] Experimental example 1 "Ji Hui 0161" Grifola frondosa facility cultivation method of the present invention comprises the following steps:
[0144] Industrialization and facility cultivation can be cultivated in a cycle for many years. Using natural conditions to cultivate in greenhouses, the suitable temperature for mycelium growth is 22-24°C, the growth temperature range for fruiting bodies is 10-30°C, and the best fruiting temperature is 16-19°C If the temperature is lower than 10°C, no primordium will appear, and if the temperature is higher than 30°C, deformed mushrooms will easily appear, and diseases will also occur.
[0145] The present invention is aimed at the method for the Grifola frondosa of " Ji Hui 0161 " facility liquid strain cultivation and comprises the following steps:
[0146] 1 Cultivation material formula
[0147] Corn cob 20%, hard miscellaneous sawdust 39%, hard miscellaneous sawdust 19%, bean curd 10%, wheat bran 10%, gypsum 1%, lime 1%;
[01...
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